Integrated Flow Chamber System for Live Cell Microscopy
In vitro quantification of the effect of mechanical loads on cells by live microscopy requires precise control of load and culture environment. Corresponding systems are often bulky, their setup and maintenance are time consuming, or the cell yield is low. Here, we show the design and initial testin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00091/full |
| _version_ | 1818260997416157184 |
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| author | Carlo Kriesi Martin Steinert Anastasios Marmaras Claudia Danzer Virginia Meskenaite Vartan Kurtcuoglu |
| author_facet | Carlo Kriesi Martin Steinert Anastasios Marmaras Claudia Danzer Virginia Meskenaite Vartan Kurtcuoglu |
| author_sort | Carlo Kriesi |
| collection | DOAJ |
| description | In vitro quantification of the effect of mechanical loads on cells by live microscopy requires precise control of load and culture environment. Corresponding systems are often bulky, their setup and maintenance are time consuming, or the cell yield is low. Here, we show the design and initial testing of a new cell culture system that fits on standard light microscope stages. Based on the parallel plate principle, the system allows for live microscopy of cells exposed to flow-induced shear stress, features short setup time and requires little user interaction. An integrated feedback-controlled heater and a bubble trap enable long observation times. The key design feature is the possibility for quick exchange of the cultured cells. We present first test results that focus on verifying the robustness, biocompatibility, and ease of use of the device. |
| first_indexed | 2024-12-12T18:40:13Z |
| format | Article |
| id | doaj.art-68296964d015491f886057736599c10e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T18:40:13Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-68296964d015491f886057736599c10e2022-12-22T00:15:41ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-05-01710.3389/fbioe.2019.00091451844Integrated Flow Chamber System for Live Cell MicroscopyCarlo Kriesi0Martin Steinert1Anastasios Marmaras2Claudia Danzer3Virginia Meskenaite4Vartan Kurtcuoglu5TrollLABS, Department of Mechanical and Industrial Engineering, Norwegian University of Science and Technology, Trondheim, NorwayTrollLABS, Department of Mechanical and Industrial Engineering, Norwegian University of Science and Technology, Trondheim, NorwayThe Interface Group, Institute of Physiology, University of Zurich, Zurich, SwitzerlandThe Interface Group, Institute of Physiology, University of Zurich, Zurich, SwitzerlandThe Interface Group, Institute of Physiology, University of Zurich, Zurich, SwitzerlandThe Interface Group, Institute of Physiology, University of Zurich, Zurich, SwitzerlandIn vitro quantification of the effect of mechanical loads on cells by live microscopy requires precise control of load and culture environment. Corresponding systems are often bulky, their setup and maintenance are time consuming, or the cell yield is low. Here, we show the design and initial testing of a new cell culture system that fits on standard light microscope stages. Based on the parallel plate principle, the system allows for live microscopy of cells exposed to flow-induced shear stress, features short setup time and requires little user interaction. An integrated feedback-controlled heater and a bubble trap enable long observation times. The key design feature is the possibility for quick exchange of the cultured cells. We present first test results that focus on verifying the robustness, biocompatibility, and ease of use of the device.https://www.frontiersin.org/article/10.3389/fbioe.2019.00091/fullcell culturemechanobiologyfluid shear stressflow chamberdeviceintegrated system |
| spellingShingle | Carlo Kriesi Martin Steinert Anastasios Marmaras Claudia Danzer Virginia Meskenaite Vartan Kurtcuoglu Integrated Flow Chamber System for Live Cell Microscopy Frontiers in Bioengineering and Biotechnology cell culture mechanobiology fluid shear stress flow chamber device integrated system |
| title | Integrated Flow Chamber System for Live Cell Microscopy |
| title_full | Integrated Flow Chamber System for Live Cell Microscopy |
| title_fullStr | Integrated Flow Chamber System for Live Cell Microscopy |
| title_full_unstemmed | Integrated Flow Chamber System for Live Cell Microscopy |
| title_short | Integrated Flow Chamber System for Live Cell Microscopy |
| title_sort | integrated flow chamber system for live cell microscopy |
| topic | cell culture mechanobiology fluid shear stress flow chamber device integrated system |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00091/full |
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