Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
<i>Streptomyces</i><i>albus</i> strain CAI-21 has been previously reported to have plant growth-promotion abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite were evaluated for their biocontrol potential against charcoal rot dis...
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MDPI AG
2020-12-01
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| Series: | Plants |
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| author | Subramaniam Gopalakrishnan Rajan Sharma Vadlamudi Srinivas Nimmala Naresh Suraj P. Mishra Sravani Ankati Sambangi Pratyusha Mahalingam Govindaraj Susana V. Gonzalez Sondre Nervik Nebojsa Simic |
| author_facet | Subramaniam Gopalakrishnan Rajan Sharma Vadlamudi Srinivas Nimmala Naresh Suraj P. Mishra Sravani Ankati Sambangi Pratyusha Mahalingam Govindaraj Susana V. Gonzalez Sondre Nervik Nebojsa Simic |
| author_sort | Subramaniam Gopalakrishnan |
| collection | DOAJ |
| description | <i>Streptomyces</i><i>albus</i> strain CAI-21 has been previously reported to have plant growth-promotion abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused by <i>Macrophomina phaseolina</i>. Results exhibited that CAI-21 significantly inhibited the growth of the pathogen, <i>M. phaseolina</i>, in dual-culture (15 mm; zone of inhibition), metabolite production (74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol potential under greenhouse and field conditions following inoculation of <i>M. phaseolina</i> by toothpick method, it significantly reduced the number of internodes infected (75% and 45% less, respectively) and length of infection (75% and 51% less, respectively) over the positive control (only <i>M. phaseolina</i> inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various chromatographic techniques, and the active compound was identified as “organophosphate” by NMR and MS. The efficacy of organophosphate was found to inhibit the growth of <i>M. phaseolina</i> in the poisoned food technique. This study indicates that <i>S.</i><i>albus</i> CAI-21 and its active metabolite organophosphate have the potential to control charcoal rot in sorghum. |
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| last_indexed | 2024-03-10T14:15:32Z |
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| spelling | doaj.art-6829b5eb4820465dbf287e11904533a52023-11-20T23:48:04ZengMDPI AGPlants2223-77472020-12-01912172710.3390/plants9121727Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of SorghumSubramaniam Gopalakrishnan0Rajan Sharma1Vadlamudi Srinivas2Nimmala Naresh3Suraj P. Mishra4Sravani Ankati5Sambangi Pratyusha6Mahalingam Govindaraj7Susana V. Gonzalez8Sondre Nervik9Nebojsa Simic10International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, IndiaDepartment of Chemistry, Faculty of Natural Sciences, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, NorwayDepartment of Chemistry, Faculty of Natural Sciences, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, NorwayDepartment of Chemistry, Faculty of Natural Sciences, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway<i>Streptomyces</i><i>albus</i> strain CAI-21 has been previously reported to have plant growth-promotion abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused by <i>Macrophomina phaseolina</i>. Results exhibited that CAI-21 significantly inhibited the growth of the pathogen, <i>M. phaseolina</i>, in dual-culture (15 mm; zone of inhibition), metabolite production (74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol potential under greenhouse and field conditions following inoculation of <i>M. phaseolina</i> by toothpick method, it significantly reduced the number of internodes infected (75% and 45% less, respectively) and length of infection (75% and 51% less, respectively) over the positive control (only <i>M. phaseolina</i> inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various chromatographic techniques, and the active compound was identified as “organophosphate” by NMR and MS. The efficacy of organophosphate was found to inhibit the growth of <i>M. phaseolina</i> in the poisoned food technique. This study indicates that <i>S.</i><i>albus</i> CAI-21 and its active metabolite organophosphate have the potential to control charcoal rot in sorghum.https://www.mdpi.com/2223-7747/9/12/1727<i>Macrophomina phaseolina</i>charcoal rotsorghumbiocontrolorganophosphate |
| spellingShingle | Subramaniam Gopalakrishnan Rajan Sharma Vadlamudi Srinivas Nimmala Naresh Suraj P. Mishra Sravani Ankati Sambangi Pratyusha Mahalingam Govindaraj Susana V. Gonzalez Sondre Nervik Nebojsa Simic Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum Plants <i>Macrophomina phaseolina</i> charcoal rot sorghum biocontrol organophosphate |
| title | Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum |
| title_full | Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum |
| title_fullStr | Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum |
| title_full_unstemmed | Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum |
| title_short | Identification and Characterization of a <i>Streptomyces</i> <i>albus</i> Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum |
| title_sort | identification and characterization of a i streptomyces i i albus i strain and its secondary metabolite organophosphate against charcoal rot of sorghum |
| topic | <i>Macrophomina phaseolina</i> charcoal rot sorghum biocontrol organophosphate |
| url | https://www.mdpi.com/2223-7747/9/12/1727 |
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