Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica
A highly sensitive ultra-performance liquid chromatography tandem mass spectrometry method for the determination of Docetaxel and metabolites (M1+M3) in human plasma samples was developed and validated. The method employs a liquid-liquid extraction and a reversed phase separation Acquity® C18 column...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
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| Series: | Química Nova |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000400474&lng=en&tlng=en |
| _version_ | 1819013821562355712 |
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| author | Suziane Raymundo Victória Vendramini Müller Mariane Tegner Andiara Artmann Natália B. Andriguetti Gilberto Schwartsmann Rafael Linden Marina V. Antunes |
| author_facet | Suziane Raymundo Victória Vendramini Müller Mariane Tegner Andiara Artmann Natália B. Andriguetti Gilberto Schwartsmann Rafael Linden Marina V. Antunes |
| author_sort | Suziane Raymundo |
| collection | DOAJ |
| description | A highly sensitive ultra-performance liquid chromatography tandem mass spectrometry method for the determination of Docetaxel and metabolites (M1+M3) in human plasma samples was developed and validated. The method employs a liquid-liquid extraction and a reversed phase separation Acquity® C18 column BEH C18 (150 x 2.1 mm, 1.7 µm). Mobile phase was a mixture of formic acid 0.1% (eluent A) and acetonitrile plus 0.1% formic acid (eluent B) in isocratic mode. Total analytical run time was 7 min. The interval of method linearity interval was 50 to 3000 ng mL-1 for docetaxel and 5.0 to 300 ng mL-1, for M1+M3 metabolites. Intra-assay and inter-assay precision was coefficient of variation 2.5-15.7% and 5.9-12.6%, respectively and accuracy was 89.0-114.0%. The method was successfully applied in the measurement of docetaxel plasma levels and area under the curve calculation in 31 cancer patients, demonstrating adequate analytical performance for the clinical application in the therapeutic drug monitoring and pharmacokinetics studies. |
| first_indexed | 2024-12-21T02:06:02Z |
| format | Article |
| id | doaj.art-682a33a605874678b406ae131a2e4de2 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-21T02:06:02Z |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-682a33a605874678b406ae131a2e4de22022-12-21T19:19:29ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-706443447447910.21577/0100-4042.20170498S0100-40422020000400474Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínicaSuziane RaymundoVictória Vendramini MüllerMariane TegnerAndiara ArtmannNatália B. AndriguettiGilberto SchwartsmannRafael LindenMarina V. AntunesA highly sensitive ultra-performance liquid chromatography tandem mass spectrometry method for the determination of Docetaxel and metabolites (M1+M3) in human plasma samples was developed and validated. The method employs a liquid-liquid extraction and a reversed phase separation Acquity® C18 column BEH C18 (150 x 2.1 mm, 1.7 µm). Mobile phase was a mixture of formic acid 0.1% (eluent A) and acetonitrile plus 0.1% formic acid (eluent B) in isocratic mode. Total analytical run time was 7 min. The interval of method linearity interval was 50 to 3000 ng mL-1 for docetaxel and 5.0 to 300 ng mL-1, for M1+M3 metabolites. Intra-assay and inter-assay precision was coefficient of variation 2.5-15.7% and 5.9-12.6%, respectively and accuracy was 89.0-114.0%. The method was successfully applied in the measurement of docetaxel plasma levels and area under the curve calculation in 31 cancer patients, demonstrating adequate analytical performance for the clinical application in the therapeutic drug monitoring and pharmacokinetics studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000400474&lng=en&tlng=endocetaxelmetabolitesvalidationtherapeutic drug monitoringlc-ms/ms |
| spellingShingle | Suziane Raymundo Victória Vendramini Müller Mariane Tegner Andiara Artmann Natália B. Andriguetti Gilberto Schwartsmann Rafael Linden Marina V. Antunes Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica Química Nova docetaxel metabolites validation therapeutic drug monitoring lc-ms/ms |
| title | Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica |
| title_full | Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica |
| title_fullStr | Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica |
| title_full_unstemmed | Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica |
| title_short | Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica |
| title_sort | determinacao de docetaxel e metabolitos em plasma por uplc ms ms desenvolvimento de metodologia analitica e aplicacao clinica |
| topic | docetaxel metabolites validation therapeutic drug monitoring lc-ms/ms |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000400474&lng=en&tlng=en |
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