Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model
Zingiber officinale is a type of Ginger used for the treatment of reactive oxygen species (ROS) associated diseases. Reports describe its use in cellular models, such as pancreatic and intestinal cancer cell lines. However, the biochemical bases of antitumor, anti-inflammatory and antioxidant activi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Journal of King Saud University: Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364716306589 |
| _version_ | 1811267198054825984 |
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| author | Angie Romero Milena Forero Luis G. Sequeda-Castañeda Adriana Grismaldo José Iglesias Crispín A. Celis-Zambrano Ingrid Schuler Ludis Morales |
| author_facet | Angie Romero Milena Forero Luis G. Sequeda-Castañeda Adriana Grismaldo José Iglesias Crispín A. Celis-Zambrano Ingrid Schuler Ludis Morales |
| author_sort | Angie Romero |
| collection | DOAJ |
| description | Zingiber officinale is a type of Ginger used for the treatment of reactive oxygen species (ROS) associated diseases. Reports describe its use in cellular models, such as pancreatic and intestinal cancer cell lines. However, the biochemical bases of antitumor, anti-inflammatory and antioxidant activities have not yet been fully elucidated. The aim of this study was to evaluate in H2O2-induced oxidative stress the effect of ginger extract (GE) on HT1080 cell viability, ROS production, AKT activation, and mitochondrial membrane potential (ΔΨm). ROS production was measured by DHE probe. Results revealed a significant cell viability decrease with increasing GE concentrations. In addition, GE at 200 μg/ml and 400 μg/ml resulted in decreased ROS production compared with controls. Moreover, GE at 200 μg/ml concentration produced a significant increase in ΔΨm. In contrast, no difference in ΔΨm was observed compared to controls with GE at 400 μg/ml. In untreated HT1080 cells basal AKT activation was not observed. Conversely, treatment with 750 μM H2O2 led to Ser473 phosphorylation. Additionally, treatment with GE at 200 μg/ml decreased AKT activation. Reports in the literature describe GE biological activities; none the less, novel approaches to investigate intracellular changes resulting from GEs are described in this study. In conclusion, this study characterized GE induced intracellular changes, leading to changes in ΔΨm and signaling protein levels, such as AKT, and reduced cell viability. Keywords: Ginger extract, ROS, Viability, AKT protein, Mitochondrial membrane potential |
| first_indexed | 2024-04-12T20:58:05Z |
| format | Article |
| id | doaj.art-682a4213979c49cf9c01cd5ae4fcf8d0 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T20:58:05Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-682a4213979c49cf9c01cd5ae4fcf8d02022-12-22T03:16:56ZengElsevierJournal of King Saud University: Science1018-36472018-04-01302263269Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell modelAngie Romero0Milena Forero1Luis G. Sequeda-Castañeda2Adriana Grismaldo3José Iglesias4Crispín A. Celis-Zambrano5Ingrid Schuler6Ludis Morales7Experimental and Computational Biochemistry Research Group, Department of Nutrition and Biochemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaExperimental and Computational Biochemistry Research Group, Department of Nutrition and Biochemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaPhytochemistry Research Group Universidad Javeriana, Department of Chemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia; Department of Pharmacy, School of Sciences, Universidad Nacional de Colombia, Bogotá, Colombia; Corresponding authors at: Experimental and Computational Biochemistry Research Group, Nutrition and Biochemistry Department, Sciences Faculty, Pontificia Universidad Javeriana, Bogotá, Colombia (L. Morales). Phytochemical Research Group Universidad Javeriana, Department of Chemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia (L.G. Sequeda-Castañeda).Experimental and Computational Biochemistry Research Group, Department of Nutrition and Biochemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaExperimental and Computational Biochemistry Research Group, Department of Nutrition and Biochemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaPhytochemistry Research Group Universidad Javeriana, Department of Chemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaBiotechnology Research Program, Sciences Faculty, Pontificia Universidad Javeriana, Bogotá, ColombiaExperimental and Computational Biochemistry Research Group, Department of Nutrition and Biochemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia; Corresponding authors at: Experimental and Computational Biochemistry Research Group, Nutrition and Biochemistry Department, Sciences Faculty, Pontificia Universidad Javeriana, Bogotá, Colombia (L. Morales). Phytochemical Research Group Universidad Javeriana, Department of Chemistry, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia (L.G. Sequeda-Castañeda).Zingiber officinale is a type of Ginger used for the treatment of reactive oxygen species (ROS) associated diseases. Reports describe its use in cellular models, such as pancreatic and intestinal cancer cell lines. However, the biochemical bases of antitumor, anti-inflammatory and antioxidant activities have not yet been fully elucidated. The aim of this study was to evaluate in H2O2-induced oxidative stress the effect of ginger extract (GE) on HT1080 cell viability, ROS production, AKT activation, and mitochondrial membrane potential (ΔΨm). ROS production was measured by DHE probe. Results revealed a significant cell viability decrease with increasing GE concentrations. In addition, GE at 200 μg/ml and 400 μg/ml resulted in decreased ROS production compared with controls. Moreover, GE at 200 μg/ml concentration produced a significant increase in ΔΨm. In contrast, no difference in ΔΨm was observed compared to controls with GE at 400 μg/ml. In untreated HT1080 cells basal AKT activation was not observed. Conversely, treatment with 750 μM H2O2 led to Ser473 phosphorylation. Additionally, treatment with GE at 200 μg/ml decreased AKT activation. Reports in the literature describe GE biological activities; none the less, novel approaches to investigate intracellular changes resulting from GEs are described in this study. In conclusion, this study characterized GE induced intracellular changes, leading to changes in ΔΨm and signaling protein levels, such as AKT, and reduced cell viability. Keywords: Ginger extract, ROS, Viability, AKT protein, Mitochondrial membrane potentialhttp://www.sciencedirect.com/science/article/pii/S1018364716306589 |
| spellingShingle | Angie Romero Milena Forero Luis G. Sequeda-Castañeda Adriana Grismaldo José Iglesias Crispín A. Celis-Zambrano Ingrid Schuler Ludis Morales Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model Journal of King Saud University: Science |
| title | Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model |
| title_full | Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model |
| title_fullStr | Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model |
| title_full_unstemmed | Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model |
| title_short | Effect of ginger extract on membrane potential changes and AKT activation on a peroxide-induced oxidative stress cell model |
| title_sort | effect of ginger extract on membrane potential changes and akt activation on a peroxide induced oxidative stress cell model |
| url | http://www.sciencedirect.com/science/article/pii/S1018364716306589 |
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