Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants
Aim: To evaluate and compare the efficiency of Triphala, Neem, the combination of Triphala, Neem and 3% sodium hypochlorite (NaOCI) in the removal of smear layer (SL) evaluated using scanning electron microscope (SEM) and antimicrobial efficacy against standard culture strains of Enterococcus faecal...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Endodontology |
| Subjects: | |
| Online Access: | http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2023;volume=35;issue=1;spage=35;epage=42;aulast=Manasa |
| _version_ | 1797824440823709696 |
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| author | Garikina Manasa Mallela G Manoj Kumar Srinivas Nallanchakrava G Naga Sri Bala Kakumanu Nageshwar Rao |
| author_facet | Garikina Manasa Mallela G Manoj Kumar Srinivas Nallanchakrava G Naga Sri Bala Kakumanu Nageshwar Rao |
| author_sort | Garikina Manasa |
| collection | DOAJ |
| description | Aim: To evaluate and compare the efficiency of Triphala, Neem, the combination of Triphala, Neem and 3% sodium hypochlorite (NaOCI) in the removal of smear layer (SL) evaluated using scanning electron microscope (SEM) and antimicrobial efficacy against standard culture strains of Enterococcus faecalis.
Methods: Seventy-five extracted human permanent teeth were divided into Group I control and Group II experimental, which was further subdivided into Group IIA, IIB, IIC, IID with 3% NaOCI, 5% Triphala extract, 7.5% Neem extract, and alternate use of Triphala and Neem and extracts as irrigants, respectively. The microbial sample was streaked on the agar plates to check colony-forming units/ml (CFU's) after inoculation and incubation at pre- and postirrigation. Teeth that were instrumented, and irrigated were split longitudinally, and examined using SEM under ×400, ×1000 to determine the debris and SL.
Results: Statistically significant reduction of CFU's was noted at postirrigation in Sub Group IIA, IIB, IIC, and IID with a mean rank of 31.77, 46.7, 34.53, and 9, respectively. SL removal was significant (P = 0.001), with Group IID exhibiting a lower mean rank, followed by B, C, A, and Group I.
Conclusion: The antimicrobial effect and SL removal efficacy were maximum for Group IID, which can be considered an effective herbal alternative in endodontic therapies. |
| first_indexed | 2024-03-13T10:38:01Z |
| format | Article |
| id | doaj.art-682b50e3a865491091085f489eeefdf5 |
| institution | Directory Open Access Journal |
| issn | 0970-7212 |
| language | English |
| last_indexed | 2024-03-13T10:38:01Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Endodontology |
| spelling | doaj.art-682b50e3a865491091085f489eeefdf52023-05-18T05:16:26ZengWolters Kluwer Medknow PublicationsEndodontology0970-72122023-01-01351354210.4103/endo.endo_146_22Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigantsGarikina ManasaMallela G Manoj KumarSrinivas NallanchakravaG Naga Sri BalaKakumanu Nageshwar RaoAim: To evaluate and compare the efficiency of Triphala, Neem, the combination of Triphala, Neem and 3% sodium hypochlorite (NaOCI) in the removal of smear layer (SL) evaluated using scanning electron microscope (SEM) and antimicrobial efficacy against standard culture strains of Enterococcus faecalis. Methods: Seventy-five extracted human permanent teeth were divided into Group I control and Group II experimental, which was further subdivided into Group IIA, IIB, IIC, IID with 3% NaOCI, 5% Triphala extract, 7.5% Neem extract, and alternate use of Triphala and Neem and extracts as irrigants, respectively. The microbial sample was streaked on the agar plates to check colony-forming units/ml (CFU's) after inoculation and incubation at pre- and postirrigation. Teeth that were instrumented, and irrigated were split longitudinally, and examined using SEM under ×400, ×1000 to determine the debris and SL. Results: Statistically significant reduction of CFU's was noted at postirrigation in Sub Group IIA, IIB, IIC, and IID with a mean rank of 31.77, 46.7, 34.53, and 9, respectively. SL removal was significant (P = 0.001), with Group IID exhibiting a lower mean rank, followed by B, C, A, and Group I. Conclusion: The antimicrobial effect and SL removal efficacy were maximum for Group IID, which can be considered an effective herbal alternative in endodontic therapies.http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2023;volume=35;issue=1;spage=35;epage=42;aulast=Manasaantimicrobial efficacyenterococcus faecalisneemsmear layertriphala |
| spellingShingle | Garikina Manasa Mallela G Manoj Kumar Srinivas Nallanchakrava G Naga Sri Bala Kakumanu Nageshwar Rao Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants Endodontology antimicrobial efficacy enterococcus faecalis neem smear layer triphala |
| title | Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| title_full | Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| title_fullStr | Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| title_full_unstemmed | Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| title_short | Evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| title_sort | evaluation of smear layer removal and antimicrobial efficacy of intracanal herbal irrigants |
| topic | antimicrobial efficacy enterococcus faecalis neem smear layer triphala |
| url | http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2023;volume=35;issue=1;spage=35;epage=42;aulast=Manasa |
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