CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos
Summary: Alternative polyadenylation (APA) gives rise to transcripts with distinct 3′ untranslated regions (3′ UTRs), thereby affecting the fate of mRNAs. APA is strongly associated with cell proliferation and differentiation status, and thus likely plays a critical role in the embryo development. H...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671122005495 |
| _version_ | 1828066603878580224 |
|---|---|
| author | Na Li Ying Cai Min Zou Jian Zhou Ling Zhang Liquan Zhou Wenpei Xiang Yan Cui Huaibiao Li |
| author_facet | Na Li Ying Cai Min Zou Jian Zhou Ling Zhang Liquan Zhou Wenpei Xiang Yan Cui Huaibiao Li |
| author_sort | Na Li |
| collection | DOAJ |
| description | Summary: Alternative polyadenylation (APA) gives rise to transcripts with distinct 3′ untranslated regions (3′ UTRs), thereby affecting the fate of mRNAs. APA is strongly associated with cell proliferation and differentiation status, and thus likely plays a critical role in the embryo development. However, the pattern of APA in mammalian early embryos is still unknown. Here, we analyzed the 3′ UTR lengths in human and mouse pre-implantation embryos using available single cell RNA-seq datasets and explored the underlying mechanism driving the changes. Although human and mouse early embryos displayed distinct patterns of 3′ UTR changing, RNA metabolism pathways were involved in both species. The 3′ UTR lengths are likely determined by the abundance of the cleavage factor I complex (CFIm) components NUDT21 and CPSF6 in the nucleus. Importantly, depletion of either component resulted in early embryo development arrest and 3′ UTR shortening. Collectively, these data highlight an essential role for APA in the development of mammalian early embryos. |
| first_indexed | 2024-04-10T23:32:51Z |
| format | Article |
| id | doaj.art-682c3ccc37984d5fbaa7c4e842e9c0d5 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-10T23:32:51Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-682c3ccc37984d5fbaa7c4e842e9c0d52023-01-12T04:19:05ZengElsevierStem Cell Reports2213-67112023-01-011818196CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryosNa Li0Ying Cai1Min Zou2Jian Zhou3Ling Zhang4Liquan Zhou5Wenpei Xiang6Yan Cui7Huaibiao Li8Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaWuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, ChinaWuhan Jianwen Biological Technology Co. LTD, Wuhan 430205, ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Corresponding authorInternational Center for Aging and Cancer, Hainan Medical University, Haikou 571199, China; Corresponding authorInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Corresponding authorSummary: Alternative polyadenylation (APA) gives rise to transcripts with distinct 3′ untranslated regions (3′ UTRs), thereby affecting the fate of mRNAs. APA is strongly associated with cell proliferation and differentiation status, and thus likely plays a critical role in the embryo development. However, the pattern of APA in mammalian early embryos is still unknown. Here, we analyzed the 3′ UTR lengths in human and mouse pre-implantation embryos using available single cell RNA-seq datasets and explored the underlying mechanism driving the changes. Although human and mouse early embryos displayed distinct patterns of 3′ UTR changing, RNA metabolism pathways were involved in both species. The 3′ UTR lengths are likely determined by the abundance of the cleavage factor I complex (CFIm) components NUDT21 and CPSF6 in the nucleus. Importantly, depletion of either component resulted in early embryo development arrest and 3′ UTR shortening. Collectively, these data highlight an essential role for APA in the development of mammalian early embryos.http://www.sciencedirect.com/science/article/pii/S2213671122005495pre-implantation embryo developmentalternative polyadenylation3′UTR lengthAPAtrapCFImNUDT21 |
| spellingShingle | Na Li Ying Cai Min Zou Jian Zhou Ling Zhang Liquan Zhou Wenpei Xiang Yan Cui Huaibiao Li CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos Stem Cell Reports pre-implantation embryo development alternative polyadenylation 3′UTR length APAtrap CFIm NUDT21 |
| title | CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos |
| title_full | CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos |
| title_fullStr | CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos |
| title_full_unstemmed | CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos |
| title_short | CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos |
| title_sort | cfim mediated alternative polyadenylation safeguards the development of mammalian pre implantation embryos |
| topic | pre-implantation embryo development alternative polyadenylation 3′UTR length APAtrap CFIm NUDT21 |
| url | http://www.sciencedirect.com/science/article/pii/S2213671122005495 |
| work_keys_str_mv | AT nali cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT yingcai cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT minzou cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT jianzhou cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT lingzhang cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT liquanzhou cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT wenpeixiang cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT yancui cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos AT huaibiaoli cfimmediatedalternativepolyadenylationsafeguardsthedevelopmentofmammalianpreimplantationembryos |