Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris
Filamentous fungi are the predominant source of lignocellulolytic enzymes used in industry for the transformation of plant biomass into high-value molecules and biofuels. The rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01002/full |
| _version_ | 1811255910604996608 |
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| author | Mireille eHaon Sacha eGrisel David eNavarro Antoine eGruet Jean-Guy eBERRIN Christophe eBignon |
| author_facet | Mireille eHaon Sacha eGrisel David eNavarro Antoine eGruet Jean-Guy eBERRIN Christophe eBignon |
| author_sort | Mireille eHaon |
| collection | DOAJ |
| description | Filamentous fungi are the predominant source of lignocellulolytic enzymes used in industry for the transformation of plant biomass into high-value molecules and biofuels. The rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for developing platforms dedicated to the recombinant expression of enzymes lacking confident functional annotation, a prerequisite to their functional and structural study. In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). This study aimed at setting-up a platform to easily and quickly screen the extracellular expression of biomass-degrading enzymes in Pichia pastoris. We first used three fungal glycoside hydrolases that we previously expressed using the protocol devised by Invitrogen to try different modifications of the original protocol. Considering the gain in time and convenience provided by the new protocol, we used it as basis to set-up the facility and produce a suite of fungal CAZymes (glycoside hydrolases, carbohydrate esterases and auxiliary activity enzyme families) out of which more than 70% were successfully expressed. The platform tasks range from gene cloning to automated protein purifications and activity tests, and is open to the CAZyme users’ community. |
| first_indexed | 2024-04-12T17:32:09Z |
| format | Article |
| id | doaj.art-682cc1a881cb4d1cbf6a5201e1ae9b44 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:32:09Z |
| publishDate | 2015-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-682cc1a881cb4d1cbf6a5201e1ae9b442022-12-22T03:23:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-09-01610.3389/fmicb.2015.01002162786Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastorisMireille eHaon0Sacha eGrisel1David eNavarro2Antoine eGruet3Jean-Guy eBERRIN4Christophe eBignon5INRAINRAINRAThe Rockefeller UniversityINRACNRSFilamentous fungi are the predominant source of lignocellulolytic enzymes used in industry for the transformation of plant biomass into high-value molecules and biofuels. The rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for developing platforms dedicated to the recombinant expression of enzymes lacking confident functional annotation, a prerequisite to their functional and structural study. In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). This study aimed at setting-up a platform to easily and quickly screen the extracellular expression of biomass-degrading enzymes in Pichia pastoris. We first used three fungal glycoside hydrolases that we previously expressed using the protocol devised by Invitrogen to try different modifications of the original protocol. Considering the gain in time and convenience provided by the new protocol, we used it as basis to set-up the facility and produce a suite of fungal CAZymes (glycoside hydrolases, carbohydrate esterases and auxiliary activity enzyme families) out of which more than 70% were successfully expressed. The platform tasks range from gene cloning to automated protein purifications and activity tests, and is open to the CAZyme users’ community.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01002/fullAutomationRecombinant ProteinsPichia pastorisglycoside hydrolaseCAZymesFactorial approach |
| spellingShingle | Mireille eHaon Sacha eGrisel David eNavarro Antoine eGruet Jean-Guy eBERRIN Christophe eBignon Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris Frontiers in Microbiology Automation Recombinant Proteins Pichia pastoris glycoside hydrolase CAZymes Factorial approach |
| title | Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris |
| title_full | Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris |
| title_fullStr | Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris |
| title_full_unstemmed | Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris |
| title_short | Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris |
| title_sort | recombinant protein production facility for fungal biomass degrading enzymes using the yeast pichia pastoris |
| topic | Automation Recombinant Proteins Pichia pastoris glycoside hydrolase CAZymes Factorial approach |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01002/full |
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