The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats

Background: Increased oxygen free radical and reduced glutathione level in the eye lens are important risk factor for cataract formation. The antioxidative property and increasing cellular and extra cellular glutathione level have been reported by several herbal medicines including silymarin.   Obj...

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Main Authors: H Fallah Huseini, A Zaree Mahmodabady, R Heshmat, M Raza
Format: Article
Language:English
Published: Institue of Medicinal Plants, ACECR 2009-02-01
Series:Journal of Medicinal Plants
Subjects:
Online Access:http://jmp.ir/article-1-529-en.html
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author H Fallah Huseini
A Zaree Mahmodabady
R Heshmat
M Raza
author_facet H Fallah Huseini
A Zaree Mahmodabady
R Heshmat
M Raza
author_sort H Fallah Huseini
collection DOAJ
description Background: Increased oxygen free radical and reduced glutathione level in the eye lens are important risk factor for cataract formation. The antioxidative property and increasing cellular and extra cellular glutathione level have been reported by several herbal medicines including silymarin.   Objective: In present interventional study Silybum marianum L. seed extract (silymarin) was tested against galactose-induced cataract development in rats.   Methods: Thirty male 45 days old wistar rats (150 – 200 g), were divided in three groups of 10 rats each. Cataract was induced in two groups of rats following feeding them with 30% galactose diet for 40 days. One group kept as control and silymarin in the dose of 200 mg/kg/d was administered orally (mixed with galactose diet) to other group for 40 days. Cataract development in the rats lens was observed daily by ophthalmoscope and naked eye during the study. The glutathione (GSH) and lipid peroxides (LPO) levels were determined after 20 days in all rats left eye lens.   Results: The results indicated that, in silymarin treated group all stage of cataract development were significantly delayed as compared to control group. In rats treated with silymarin the lens GSH level was increased significantly (p<0.01) and LPO levels was decreased significantly as compared to control group (p<0.05). Conclusion: Administration of silymarin to galactose fed rats showed beneficial effect on prevention of cataract development as well as antioxidative defence system such as increase in lens GSH and decrease LPO levels.
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spelling doaj.art-682ccc6af0c04803899c1d139f42098f2022-12-21T23:24:58ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582009-02-01829712The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in RatsH Fallah Huseini0A Zaree Mahmodabady1R Heshmat2M Raza3 Department of Pharmacology, Institute of Medicinal Plants ACECR, Tehran Department of Biochemistry, Faculty of Medicine, Baqiyatallah (a.s.) University of Medical Sciences, Tehran Endocrinology & Metabolism Research Center, Tehran University of Medical Sciences Tehran Applied Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran Background: Increased oxygen free radical and reduced glutathione level in the eye lens are important risk factor for cataract formation. The antioxidative property and increasing cellular and extra cellular glutathione level have been reported by several herbal medicines including silymarin.   Objective: In present interventional study Silybum marianum L. seed extract (silymarin) was tested against galactose-induced cataract development in rats.   Methods: Thirty male 45 days old wistar rats (150 – 200 g), were divided in three groups of 10 rats each. Cataract was induced in two groups of rats following feeding them with 30% galactose diet for 40 days. One group kept as control and silymarin in the dose of 200 mg/kg/d was administered orally (mixed with galactose diet) to other group for 40 days. Cataract development in the rats lens was observed daily by ophthalmoscope and naked eye during the study. The glutathione (GSH) and lipid peroxides (LPO) levels were determined after 20 days in all rats left eye lens.   Results: The results indicated that, in silymarin treated group all stage of cataract development were significantly delayed as compared to control group. In rats treated with silymarin the lens GSH level was increased significantly (p<0.01) and LPO levels was decreased significantly as compared to control group (p<0.05). Conclusion: Administration of silymarin to galactose fed rats showed beneficial effect on prevention of cataract development as well as antioxidative defence system such as increase in lens GSH and decrease LPO levels.http://jmp.ir/article-1-529-en.htmlcataractsilymarinantioxidantherbal medicinegalactose
spellingShingle H Fallah Huseini
A Zaree Mahmodabady
R Heshmat
M Raza
The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
Journal of Medicinal Plants
cataract
silymarin
antioxidant
herbal medicine
galactose
title The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
title_full The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
title_fullStr The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
title_full_unstemmed The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
title_short The Effect of Silybum marianum (L.) Gaertn. Seed Extract (Silymarin) on Galactose Induced Cataract Formation in Rats
title_sort effect of silybum marianum l gaertn seed extract silymarin on galactose induced cataract formation in rats
topic cataract
silymarin
antioxidant
herbal medicine
galactose
url http://jmp.ir/article-1-529-en.html
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