Effect of insulin-like growth factor system on luteinising angiogenesis
Preovulatory follicle growth and the luteal transition require intense angiogenesis. This enables progesterone production to increase sufficiently to support a pregnancy. Inadequate follicular or luteal vascularisation can lead to reduced ovarian function and thus compromise fertility. Insulin-like...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2023-01-01
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| Series: | Reproduction and Fertility |
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| Online Access: | https://raf.bioscientifica.com/view/journals/raf/4/2/RAF-22-0057.xml |
| _version_ | 1797840341660860416 |
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| author | Chinwe U Nwachukwu Robert S Robinson Kathryn J Woad |
| author_facet | Chinwe U Nwachukwu Robert S Robinson Kathryn J Woad |
| author_sort | Chinwe U Nwachukwu |
| collection | DOAJ |
| description | Preovulatory follicle growth and the luteal transition require intense angiogenesis. This enables progesterone production to increase sufficiently to support a pregnancy. Inadequate follicular or luteal vascularisation can lead to reduced ovarian function and thus compromise fertility. Insulin-like growth factor 1 (IGF1) and IGF2 regulate multiple ovarian processes and are key links between an animal’s reproductive and metabolic status. This study investigated the role that the IGF system plays in regulating luteinising follicular endothelial cell (EC) networks and progesterone production in vitro. Bovine luteinising follicular angiogenesis cultures were treated with (i) LR3-IGF1 (10 or 100 ng/mL) under basal and angiogenic-stimulated conditions or (ii) IGF1 receptor (IGF1R) inhibitor (picropodophyllin (PPP); 1 μM) in the presence or absence of LR3-IGF1, IGF2 or combined LR3-IGF1 + IGF2 (10 ng/mL). EC networks were quantified by von Willebrand factor immunohistochemistry. Progesterone production was analysed by ELISA, and cell proliferation was determined by MTT assay. LR3-IGF1 had limited effects on EC growth parameters, whilst PPP (P < 0.001) markedly reduced EC growth parameters (by 60–70%). Cell proliferation was slightly increased (by 3–5%) by LR3-IGF1 (P < 0.001). LR3-IGF1 had variable effects on progesterone production, whilst PPP reduced progesterone concentration (P < 0.001) with or without LR3- IGF1 or IGF2 alone or in combination. IGF1 was detected in cell-conditioned media and was increased by LH (50 ng/ mL) (P < 0.001). In conclusion, exogenous IGF1 and IGF2 had minimal effects on luteinising follicular angiogenesis and progesterone production, but the inhibitory effect of the IGF1R inhibitor (PPP) suggests that IGF1R signalling is critical for the development of EC networks and progesterone production in luteinising follicular cells. |
| first_indexed | 2024-04-09T16:13:22Z |
| format | Article |
| id | doaj.art-682d799c81dd49ac9e939118e8a1ed49 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-04-09T16:13:22Z |
| publishDate | 2023-01-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-682d799c81dd49ac9e939118e8a1ed492023-04-24T07:23:37ZengBioscientificaReproduction and Fertility2633-83862023-01-0142112https://doi.org/10.1530/RAF-22-0057Effect of insulin-like growth factor system on luteinising angiogenesisChinwe U Nwachukwu0Robert S Robinson1Kathryn J Woad2School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington campus, Loughborough, United Kingdom; Department of Agricultural Science, School of Agriculture and Vocational Studies, Alvan Ikoku Federal College of Education, Owerri, Imo State, Nigeria School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington campus, Loughborough, United KingdomSchool of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington campus, Loughborough, United KingdomPreovulatory follicle growth and the luteal transition require intense angiogenesis. This enables progesterone production to increase sufficiently to support a pregnancy. Inadequate follicular or luteal vascularisation can lead to reduced ovarian function and thus compromise fertility. Insulin-like growth factor 1 (IGF1) and IGF2 regulate multiple ovarian processes and are key links between an animal’s reproductive and metabolic status. This study investigated the role that the IGF system plays in regulating luteinising follicular endothelial cell (EC) networks and progesterone production in vitro. Bovine luteinising follicular angiogenesis cultures were treated with (i) LR3-IGF1 (10 or 100 ng/mL) under basal and angiogenic-stimulated conditions or (ii) IGF1 receptor (IGF1R) inhibitor (picropodophyllin (PPP); 1 μM) in the presence or absence of LR3-IGF1, IGF2 or combined LR3-IGF1 + IGF2 (10 ng/mL). EC networks were quantified by von Willebrand factor immunohistochemistry. Progesterone production was analysed by ELISA, and cell proliferation was determined by MTT assay. LR3-IGF1 had limited effects on EC growth parameters, whilst PPP (P < 0.001) markedly reduced EC growth parameters (by 60–70%). Cell proliferation was slightly increased (by 3–5%) by LR3-IGF1 (P < 0.001). LR3-IGF1 had variable effects on progesterone production, whilst PPP reduced progesterone concentration (P < 0.001) with or without LR3- IGF1 or IGF2 alone or in combination. IGF1 was detected in cell-conditioned media and was increased by LH (50 ng/ mL) (P < 0.001). In conclusion, exogenous IGF1 and IGF2 had minimal effects on luteinising follicular angiogenesis and progesterone production, but the inhibitory effect of the IGF1R inhibitor (PPP) suggests that IGF1R signalling is critical for the development of EC networks and progesterone production in luteinising follicular cells.https://raf.bioscientifica.com/view/journals/raf/4/2/RAF-22-0057.xmlinsulin-like growth factors (igfs)endothelial cellsigf1 receptor inhibitorangiogenesisbovine |
| spellingShingle | Chinwe U Nwachukwu Robert S Robinson Kathryn J Woad Effect of insulin-like growth factor system on luteinising angiogenesis Reproduction and Fertility insulin-like growth factors (igfs) endothelial cells igf1 receptor inhibitor angiogenesis bovine |
| title | Effect of insulin-like growth factor system on luteinising angiogenesis |
| title_full | Effect of insulin-like growth factor system on luteinising angiogenesis |
| title_fullStr | Effect of insulin-like growth factor system on luteinising angiogenesis |
| title_full_unstemmed | Effect of insulin-like growth factor system on luteinising angiogenesis |
| title_short | Effect of insulin-like growth factor system on luteinising angiogenesis |
| title_sort | effect of insulin like growth factor system on luteinising angiogenesis |
| topic | insulin-like growth factors (igfs) endothelial cells igf1 receptor inhibitor angiogenesis bovine |
| url | https://raf.bioscientifica.com/view/journals/raf/4/2/RAF-22-0057.xml |
| work_keys_str_mv | AT chinweunwachukwu effectofinsulinlikegrowthfactorsystemonluteinisingangiogenesis AT robertsrobinson effectofinsulinlikegrowthfactorsystemonluteinisingangiogenesis AT kathrynjwoad effectofinsulinlikegrowthfactorsystemonluteinisingangiogenesis |