Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe
Fluorescence imaging is a powerful technique for continuous observation of dynamic intracellular processes of living cells. Fluorescent probes bearing a fluorescence switching property associated with a specific recognition or reaction of target biomolecule, that is, stimuli-responsibility, are impo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/21/7181 |
| _version_ | 1797467104691093504 |
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| author | Eiji Nakata Khongorzul Gerelbaatar Futa Komatsubara Takashi Morii |
| author_facet | Eiji Nakata Khongorzul Gerelbaatar Futa Komatsubara Takashi Morii |
| author_sort | Eiji Nakata |
| collection | DOAJ |
| description | Fluorescence imaging is a powerful technique for continuous observation of dynamic intracellular processes of living cells. Fluorescent probes bearing a fluorescence switching property associated with a specific recognition or reaction of target biomolecule, that is, stimuli-responsibility, are important for fluorescence imaging. Thus, fluorescent probes continue to be developed to support approaches with different design strategies. When compared with simple intensity-changing fluorescent probes, ratiometric fluorescent probes typically offer the advantage of less sensitivity to errors associated with probe concentration, photobleaching, and environmental effects. For intracellular usage, ratiometric fluorescent probes based on small molecules must be loaded into the cells. Thus, probes having intrinsic fluorescence may obscure a change in intracellular signal if the background fluorescence of the remaining extracellular probes is high. To overcome such disadvantages, it is necessary to minimize the extracellular background fluorescence of fluorescent probes. Here, the design strategy of the latent ratiometric fluorescent probe for wash-free ratiometric imaging using a xanthene dye seminapthorhodafluor (SNARF) as the scaffold of fluorophore is discussed. |
| first_indexed | 2024-03-09T18:48:54Z |
| format | Article |
| id | doaj.art-68318b7af9194bcc846fb0fe40d0936d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:48:54Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-68318b7af9194bcc846fb0fe40d0936d2023-11-24T05:59:55ZengMDPI AGMolecules1420-30492022-10-012721718110.3390/molecules27217181Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent ProbeEiji Nakata0Khongorzul Gerelbaatar1Futa Komatsubara2Takashi Morii3Institute of Advanced Energy, Kyoto University, Uji 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Uji 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Uji 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Uji 611-0011, JapanFluorescence imaging is a powerful technique for continuous observation of dynamic intracellular processes of living cells. Fluorescent probes bearing a fluorescence switching property associated with a specific recognition or reaction of target biomolecule, that is, stimuli-responsibility, are important for fluorescence imaging. Thus, fluorescent probes continue to be developed to support approaches with different design strategies. When compared with simple intensity-changing fluorescent probes, ratiometric fluorescent probes typically offer the advantage of less sensitivity to errors associated with probe concentration, photobleaching, and environmental effects. For intracellular usage, ratiometric fluorescent probes based on small molecules must be loaded into the cells. Thus, probes having intrinsic fluorescence may obscure a change in intracellular signal if the background fluorescence of the remaining extracellular probes is high. To overcome such disadvantages, it is necessary to minimize the extracellular background fluorescence of fluorescent probes. Here, the design strategy of the latent ratiometric fluorescent probe for wash-free ratiometric imaging using a xanthene dye seminapthorhodafluor (SNARF) as the scaffold of fluorophore is discussed.https://www.mdpi.com/1420-3049/27/21/7181self-assembled fluorophorelatent fluorescent proberatiometrywash-freestimuli-responsibility |
| spellingShingle | Eiji Nakata Khongorzul Gerelbaatar Futa Komatsubara Takashi Morii Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe Molecules self-assembled fluorophore latent fluorescent probe ratiometry wash-free stimuli-responsibility |
| title | Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe |
| title_full | Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe |
| title_fullStr | Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe |
| title_full_unstemmed | Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe |
| title_short | Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe |
| title_sort | stimuli responsible snarf derivatives as a latent ratiometric fluorescent probe |
| topic | self-assembled fluorophore latent fluorescent probe ratiometry wash-free stimuli-responsibility |
| url | https://www.mdpi.com/1420-3049/27/21/7181 |
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