Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd.
The aim of this work was to study the chemical composition, antioxidant and antibacterial properties of Achillea distans Waldst. et Kit. subsp. distans and Achillea distans Waldst. et Kit. subsp. alpina Rochel, from the Rodna Mountains (Romania). The identification and quantification of major phenol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/8/8725 |
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| author | Radu Silaghi-Dumitrescu Daniela Hanganu Grigore Damian Ilioara Oniga Daniela Benedec Augustin C. Mot Laurian Vlase Mihaela Duma |
| author_facet | Radu Silaghi-Dumitrescu Daniela Hanganu Grigore Damian Ilioara Oniga Daniela Benedec Augustin C. Mot Laurian Vlase Mihaela Duma |
| author_sort | Radu Silaghi-Dumitrescu |
| collection | DOAJ |
| description | The aim of this work was to study the chemical composition, antioxidant and antibacterial properties of Achillea distans Waldst. et Kit. subsp. distans and Achillea distans Waldst. et Kit. subsp. alpina Rochel, from the Rodna Mountains (Romania). The identification and quantification of major phenolic compounds was performed by a HPLC-MS method. The total polyphenolic and flavonoid content was determined spectrophotometrically. The antioxidant activity was evaluated using the DPPH bleaching method, trolox equivalent antioxidant capacity assay (TEAC), hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and an Electron Paramagnetic Resonance (EPR) spectroscopy method. A data indicated that A. distans subsp. alpina extract has more antioxidant activity than A. distans subsp. distans extract. Luteolin, apigenin, quercetin, caffeic and chlorogenic acids were present in the two extracts of A. distans, but in different amounts. Three flavonoids were detected only in A. distans subsp. alpina. The polyphenol-richer A. distans subsp. alpina extract showed a higher antioxidant activity than A. distans subsp. distans extract. A. distans subsp. distans extract showed inhibitory activity for Gram-positive bacteria, as evaluated with four species. The quantitative and qualitative differences between the two subspecies of Achillea distans could be used as a potential taxonomic marker in order to distinguish the species. |
| first_indexed | 2024-04-12T19:24:56Z |
| format | Article |
| id | doaj.art-6832cfabd8ba4556bf0f9bd66827062a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T19:24:56Z |
| publishDate | 2013-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6832cfabd8ba4556bf0f9bd66827062a2022-12-22T03:19:30ZengMDPI AGMolecules1420-30492013-07-011888725873910.3390/molecules18088725Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd.Radu Silaghi-DumitrescuDaniela HanganuGrigore DamianIlioara OnigaDaniela BenedecAugustin C. MotLaurian VlaseMihaela DumaThe aim of this work was to study the chemical composition, antioxidant and antibacterial properties of Achillea distans Waldst. et Kit. subsp. distans and Achillea distans Waldst. et Kit. subsp. alpina Rochel, from the Rodna Mountains (Romania). The identification and quantification of major phenolic compounds was performed by a HPLC-MS method. The total polyphenolic and flavonoid content was determined spectrophotometrically. The antioxidant activity was evaluated using the DPPH bleaching method, trolox equivalent antioxidant capacity assay (TEAC), hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and an Electron Paramagnetic Resonance (EPR) spectroscopy method. A data indicated that A. distans subsp. alpina extract has more antioxidant activity than A. distans subsp. distans extract. Luteolin, apigenin, quercetin, caffeic and chlorogenic acids were present in the two extracts of A. distans, but in different amounts. Three flavonoids were detected only in A. distans subsp. alpina. The polyphenol-richer A. distans subsp. alpina extract showed a higher antioxidant activity than A. distans subsp. distans extract. A. distans subsp. distans extract showed inhibitory activity for Gram-positive bacteria, as evaluated with four species. The quantitative and qualitative differences between the two subspecies of Achillea distans could be used as a potential taxonomic marker in order to distinguish the species.http://www.mdpi.com/1420-3049/18/8/8725Achillea distanspolyphenolsantioxidant activityhemoglobinEPR |
| spellingShingle | Radu Silaghi-Dumitrescu Daniela Hanganu Grigore Damian Ilioara Oniga Daniela Benedec Augustin C. Mot Laurian Vlase Mihaela Duma Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. Molecules Achillea distans polyphenols antioxidant activity hemoglobin EPR |
| title | Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. |
| title_full | Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. |
| title_fullStr | Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. |
| title_full_unstemmed | Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. |
| title_short | Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd. |
| title_sort | polyphenolic composition antioxidant and antibacterial activities for two romanian subspecies of achillea distans waldst et kit ex willd |
| topic | Achillea distans polyphenols antioxidant activity hemoglobin EPR |
| url | http://www.mdpi.com/1420-3049/18/8/8725 |
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