Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors
Jasmonate ZIM-domain (JAZ) family proteins are the key repressors in the jasmonate signaling pathway and play crucial roles in plant development, defenses, and responses to stresses. However, our knowledge about the JAZ protein family in petunia is limited. This research respectively identified 12 a...
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MDPI AG
2019-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/7/203 |
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| author | Shaoze Tian Siyu Liu Yu Wang Kun Wang Chaoqun Yin Yuanzheng Yue Huirong Hu |
| author_facet | Shaoze Tian Siyu Liu Yu Wang Kun Wang Chaoqun Yin Yuanzheng Yue Huirong Hu |
| author_sort | Shaoze Tian |
| collection | DOAJ |
| description | Jasmonate ZIM-domain (JAZ) family proteins are the key repressors in the jasmonate signaling pathway and play crucial roles in plant development, defenses, and responses to stresses. However, our knowledge about the JAZ protein family in petunia is limited. This research respectively identified 12 and 16 JAZ proteins in two <i>Petunia</i> progenitors, <i>Petunia axillaris</i> and <i>Petunia inflata</i>. Phylogenetic analysis showed that the 28 proteins could be divided into four groups (Groups A−D) and further classified into six subgroups (A1, A2, B1, B3, C, and D1); members in the same subgroup shared some similarities in motif composition and sequence structure. The Ka/Ks ratios of seven paralogous pairs were less than one, suggesting the petunia JAZ family might have principally undergone purifying selection. Quantitative real-time PCR (qRT-PCR) analysis revealed that <i>PaJAZ</i> genes presented differential expression patterns during the development of flower bud and anther in petunia, and the expression of <i>PaJAZ5</i>, <i>9</i>, <i>12</i> genes was generally up-regulated after MeJA treatment. Subcellular localization assays demonstrated that proteins PaJAZ5, 9, 12 were localized in nucleus. Yeast two hybrid (Y2H) elucidated most PaJAZ proteins (PaJAZ1-7, 9, 12) might interact with transcription factor MYC2. This study provides insights for further investigation of functional analysis in petunia JAZ family proteins. |
| first_indexed | 2024-12-20T11:23:05Z |
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| language | English |
| last_indexed | 2024-12-20T11:23:05Z |
| publishDate | 2019-07-01 |
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| series | Plants |
| spelling | doaj.art-68332f45b52c435990757741600a56ce2022-12-21T19:42:27ZengMDPI AGPlants2223-77472019-07-018720310.3390/plants8070203plants8070203Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> ProgenitorsShaoze Tian0Siyu Liu1Yu Wang2Kun Wang3Chaoqun Yin4Yuanzheng Yue5Huirong Hu6Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaJasmonate ZIM-domain (JAZ) family proteins are the key repressors in the jasmonate signaling pathway and play crucial roles in plant development, defenses, and responses to stresses. However, our knowledge about the JAZ protein family in petunia is limited. This research respectively identified 12 and 16 JAZ proteins in two <i>Petunia</i> progenitors, <i>Petunia axillaris</i> and <i>Petunia inflata</i>. Phylogenetic analysis showed that the 28 proteins could be divided into four groups (Groups A−D) and further classified into six subgroups (A1, A2, B1, B3, C, and D1); members in the same subgroup shared some similarities in motif composition and sequence structure. The Ka/Ks ratios of seven paralogous pairs were less than one, suggesting the petunia JAZ family might have principally undergone purifying selection. Quantitative real-time PCR (qRT-PCR) analysis revealed that <i>PaJAZ</i> genes presented differential expression patterns during the development of flower bud and anther in petunia, and the expression of <i>PaJAZ5</i>, <i>9</i>, <i>12</i> genes was generally up-regulated after MeJA treatment. Subcellular localization assays demonstrated that proteins PaJAZ5, 9, 12 were localized in nucleus. Yeast two hybrid (Y2H) elucidated most PaJAZ proteins (PaJAZ1-7, 9, 12) might interact with transcription factor MYC2. This study provides insights for further investigation of functional analysis in petunia JAZ family proteins.https://www.mdpi.com/2223-7747/8/7/203JAZjasmonatepetuniaphylogenetic analysisqRT-PCRantherMYC2 |
| spellingShingle | Shaoze Tian Siyu Liu Yu Wang Kun Wang Chaoqun Yin Yuanzheng Yue Huirong Hu Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors Plants JAZ jasmonate petunia phylogenetic analysis qRT-PCR anther MYC2 |
| title | Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors |
| title_full | Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors |
| title_fullStr | Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors |
| title_full_unstemmed | Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors |
| title_short | Genome-Wide Identification and Characterization of JAZ Protein Family in Two <i>Petunia</i> Progenitors |
| title_sort | genome wide identification and characterization of jaz protein family in two i petunia i progenitors |
| topic | JAZ jasmonate petunia phylogenetic analysis qRT-PCR anther MYC2 |
| url | https://www.mdpi.com/2223-7747/8/7/203 |
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