Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects
Over the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated and resulting in various biological effects, most of them after long exposure or treat...
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| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/13/7803 |
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| author | Alexandru Bogdan Stache Ilarion Mihăilă Ioana Cristina Gerber Loredana Mihaiela Dragoș Cosmin Teodor Mihai Iuliu Cristian Ivanov Ionuț Topală Dragoș-Lucian Gorgan |
| author_facet | Alexandru Bogdan Stache Ilarion Mihăilă Ioana Cristina Gerber Loredana Mihaiela Dragoș Cosmin Teodor Mihai Iuliu Cristian Ivanov Ionuț Topală Dragoș-Lucian Gorgan |
| author_sort | Alexandru Bogdan Stache |
| collection | DOAJ |
| description | Over the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated and resulting in various biological effects, most of them after long exposure or treatment durations. This study aimed to evaluate the potential of a custom-made CAP generation source to produce plasma-activated medium (PAM) with cytotoxic effects and subsequently to establish the optimal exposure and treatment parameters. The exposure’s electrical parameters, as well as pH and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>NO</mi></mrow><mn>2</mn><mo>−</mo></msubsup></mrow></semantics></math></inline-formula> content of PAM were analyzed. The cytotoxic potential and optimal parameters of the treatment were established by evaluating the viability of human osteosarcoma cells (HOS cell line) and human osteoblasts (HOB cell line) treated with PAM under different conditions. Our results showed that indirect treatment with CAP presents selective dose-dependent cytotoxic effects, while the cell viability decrease was not found to be correlated with the PAM acidification due to CAP exposure. The Griess assay revealed very high and long-term stable concentrations of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>NO</mi></mrow><mn>2</mn><mo>−</mo></msubsup></mrow></semantics></math></inline-formula> in PAM. Overall, this study presents a simple and faster method of producing PAM treatment with cytotoxic effects on HOS cells, by using a custom-built CAP source. |
| first_indexed | 2024-03-11T01:47:05Z |
| format | Article |
| id | doaj.art-68373fc55eda43d9accb1720787df36b |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T01:47:05Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-68373fc55eda43d9accb1720787df36b2023-11-18T16:11:41ZengMDPI AGApplied Sciences2076-34172023-07-011313780310.3390/app13137803Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic EffectsAlexandru Bogdan Stache0Ilarion Mihăilă1Ioana Cristina Gerber2Loredana Mihaiela Dragoș3Cosmin Teodor Mihai4Iuliu Cristian Ivanov5Ionuț Topală6Dragoș-Lucian Gorgan7Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700505 Iasi, RomaniaIntegrated Center of Environmental Science Studies in the North-Eastern Development Region (CERNESIM), Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaIntegrated Center of Environmental Science Studies in the North-Eastern Development Region (CERNESIM), Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaMolecular Diagnosis Department, Regional Institute of Oncology, 700483 Iasi, RomaniaPraxis Medical Investigations, 700376 Iasi, RomaniaMolecular Diagnosis Department, Regional Institute of Oncology, 700483 Iasi, RomaniaFaculty of Physics, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaFaculty of Biology, Alexandru Ioan Cuza University of Iasi, 700505 Iasi, RomaniaOver the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated and resulting in various biological effects, most of them after long exposure or treatment durations. This study aimed to evaluate the potential of a custom-made CAP generation source to produce plasma-activated medium (PAM) with cytotoxic effects and subsequently to establish the optimal exposure and treatment parameters. The exposure’s electrical parameters, as well as pH and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>NO</mi></mrow><mn>2</mn><mo>−</mo></msubsup></mrow></semantics></math></inline-formula> content of PAM were analyzed. The cytotoxic potential and optimal parameters of the treatment were established by evaluating the viability of human osteosarcoma cells (HOS cell line) and human osteoblasts (HOB cell line) treated with PAM under different conditions. Our results showed that indirect treatment with CAP presents selective dose-dependent cytotoxic effects, while the cell viability decrease was not found to be correlated with the PAM acidification due to CAP exposure. The Griess assay revealed very high and long-term stable concentrations of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>NO</mi></mrow><mn>2</mn><mo>−</mo></msubsup></mrow></semantics></math></inline-formula> in PAM. Overall, this study presents a simple and faster method of producing PAM treatment with cytotoxic effects on HOS cells, by using a custom-built CAP source.https://www.mdpi.com/2076-3417/13/13/7803cold atmospheric plasmadielectric barrier dischargeindirect treatmentosteosarcomaPAM |
| spellingShingle | Alexandru Bogdan Stache Ilarion Mihăilă Ioana Cristina Gerber Loredana Mihaiela Dragoș Cosmin Teodor Mihai Iuliu Cristian Ivanov Ionuț Topală Dragoș-Lucian Gorgan Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects Applied Sciences cold atmospheric plasma dielectric barrier discharge indirect treatment osteosarcoma PAM |
| title | Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects |
| title_full | Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects |
| title_fullStr | Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects |
| title_full_unstemmed | Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects |
| title_short | Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects |
| title_sort | optimization of indirect cap exposure as an effective osteosarcoma cells treatment with cytotoxic effects |
| topic | cold atmospheric plasma dielectric barrier discharge indirect treatment osteosarcoma PAM |
| url | https://www.mdpi.com/2076-3417/13/13/7803 |
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