P53 codon 11, 72, and 248 gene polymorphisms in endometriosis
<p>Objective: Mutated p53 gene is related to the instability of cell growth and cell cycle progression. We aimed to evaluate the association between endometriosis and p53 codon 11, 72 and 248 gene polymorphisms.</p> <p>Patients and methods: Women were divided into two groups: (1) m...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2006-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v02p0188.htm |
| _version_ | 1819049778737053696 |
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| author | Yao-Yuan Hsieh , Chich-Sheng Lin |
| author_facet | Yao-Yuan Hsieh , Chich-Sheng Lin |
| author_sort | Yao-Yuan Hsieh , Chich-Sheng Lin |
| collection | DOAJ |
| description | <p>Objective: Mutated p53 gene is related to the instability of cell growth and cell cycle progression. We aimed to evaluate the association between endometriosis and p53 codon 11, 72 and 248 gene polymorphisms.</p> <p>Patients and methods: Women were divided into two groups: (1) moderate/severe endometriosis (n=148), and (2) non-endometriosis groups (n=150). P53 gene polymorphisms include codon11 Glu/Gln or Lys (GAG->CAG or AAG), codon 72 Arg/Pro (CGC->CCC), and codon 248 Arg/Thr (CGG->TCG). These gene polymorphisms were amplified by polymerase chain reaction and detected by electrophoresis after restriction enzyme (<i>Taq </i>I, <i>BstU</i> I, <i>Hap </i>II) digestions. Associations between the endometriosis and p53 polymorphisms were evaluated.</p> <p>Results: The distributions of p53 codon 72 polymorphisms in both groups were significantly different. The proportions of Arg homozygotes/heterozygotes/Pro homozygotes in both groups were 9.5/66.2/24.3% and 30.7/50/19.3%. The proportions of Arg/Pro alleles were 42.6/57.4% and 56/44%. The distributions of p53 codon 11 and 248 polymorphisms in both groups were non-significantly different. All individuals appeared the wild genotypes (Glu11 and Arg248 homozygotes).</p> <p>Conclusion: Association between endometriosis and p53 codon 72 polymorphism exists. P53 codon 72*Pro-related genotype and allele are related with higher susceptibility of endometriosis. P53 codon 11 and 248 polymorphisms are not related with endometriosis susceptibility.</p> |
| first_indexed | 2024-12-21T11:37:34Z |
| format | Article |
| id | doaj.art-6839443aa1c14586a50270e739646c72 |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-21T11:37:34Z |
| publishDate | 2006-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-6839443aa1c14586a50270e739646c722022-12-21T19:05:25ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882006-01-0124188193P53 codon 11, 72, and 248 gene polymorphisms in endometriosisYao-Yuan Hsieh , Chich-Sheng Lin<p>Objective: Mutated p53 gene is related to the instability of cell growth and cell cycle progression. We aimed to evaluate the association between endometriosis and p53 codon 11, 72 and 248 gene polymorphisms.</p> <p>Patients and methods: Women were divided into two groups: (1) moderate/severe endometriosis (n=148), and (2) non-endometriosis groups (n=150). P53 gene polymorphisms include codon11 Glu/Gln or Lys (GAG->CAG or AAG), codon 72 Arg/Pro (CGC->CCC), and codon 248 Arg/Thr (CGG->TCG). These gene polymorphisms were amplified by polymerase chain reaction and detected by electrophoresis after restriction enzyme (<i>Taq </i>I, <i>BstU</i> I, <i>Hap </i>II) digestions. Associations between the endometriosis and p53 polymorphisms were evaluated.</p> <p>Results: The distributions of p53 codon 72 polymorphisms in both groups were significantly different. The proportions of Arg homozygotes/heterozygotes/Pro homozygotes in both groups were 9.5/66.2/24.3% and 30.7/50/19.3%. The proportions of Arg/Pro alleles were 42.6/57.4% and 56/44%. The distributions of p53 codon 11 and 248 polymorphisms in both groups were non-significantly different. All individuals appeared the wild genotypes (Glu11 and Arg248 homozygotes).</p> <p>Conclusion: Association between endometriosis and p53 codon 72 polymorphism exists. P53 codon 72*Pro-related genotype and allele are related with higher susceptibility of endometriosis. P53 codon 11 and 248 polymorphisms are not related with endometriosis susceptibility.</p>http://www.biolsci.org/v02p0188.htm |
| spellingShingle | Yao-Yuan Hsieh , Chich-Sheng Lin P53 codon 11, 72, and 248 gene polymorphisms in endometriosis International Journal of Biological Sciences |
| title | P53 codon 11, 72, and 248 gene polymorphisms in endometriosis |
| title_full | P53 codon 11, 72, and 248 gene polymorphisms in endometriosis |
| title_fullStr | P53 codon 11, 72, and 248 gene polymorphisms in endometriosis |
| title_full_unstemmed | P53 codon 11, 72, and 248 gene polymorphisms in endometriosis |
| title_short | P53 codon 11, 72, and 248 gene polymorphisms in endometriosis |
| title_sort | p53 codon 11 72 and 248 gene polymorphisms in endometriosis |
| url | http://www.biolsci.org/v02p0188.htm |
| work_keys_str_mv | AT yaoyuanhsiehchichshenglin p53codon1172and248genepolymorphismsinendometriosis |