Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis
Abstract Background Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. ba...
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| Language: | English |
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BMC
2020-04-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01792-w |
| _version_ | 1818501064301740032 |
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| author | Ali Mohammadi Bardbari Parviz Mohajeri Mohammad Reza Arabestani Manoochehr Karami Fariba Keramat Saba Asadollahi Amir Khodavirdipour Mohammad Yousef Alikhani |
| author_facet | Ali Mohammadi Bardbari Parviz Mohajeri Mohammad Reza Arabestani Manoochehr Karami Fariba Keramat Saba Asadollahi Amir Khodavirdipour Mohammad Yousef Alikhani |
| author_sort | Ali Mohammadi Bardbari |
| collection | DOAJ |
| description | Abstract Background Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. Forty-three clinical and 26 environmental isolates of the MDR A. baumannii were collected and recognized via API 20NE. Antibiotic resistance of the isolates was assessed by the disk diffusion method, and the biofilm formation test was done by the microtiter plate method. Pulsed Field Gel Electrophoresis (PFGE) was used to assess the genomic features of the bacterial isolates. Results The resistance rate of clinical and environmental isolates against antibiotics were from 95 to 100%. The difference in antibiotic resistance rates between clinical and environmental isolates was not statistically significant (p > 0.05). Biofilm production capabilities revealed that 31 (44.9%), and 30 (43.5%) isolates had strong and moderate biofilm producer activity, respectively. PFGE typing exhibited eight different clusters (A, B, C, D, E, F, G, and H) with two significant clusters included A and G with 21 (30.4%) and 16 (23.2%) members respectively, which comprises up to 53.6% of all isolates. There was no relationship between biofilm formation and antibiotic resistance patterns with PFGE pulsotypes. Conclusions The results show that there is a close relationship between environmental and clinical isolates of A. baumannii. Cross-contamination is also very important that occurs through daily clinical activities between environmental and clinical isolates. Therefore, in order to reduce the clonal contamination of MDR A. baumannii environmental and clinical isolates, it is necessary to use strict infection control strategies. |
| first_indexed | 2024-12-10T20:51:07Z |
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| id | doaj.art-6839a64533394cd9929dd591122def6c |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T20:51:07Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-6839a64533394cd9929dd591122def6c2022-12-22T01:34:05ZengBMCBMC Microbiology1471-21802020-04-012011710.1186/s12866-020-01792-wMolecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresisAli Mohammadi Bardbari0Parviz Mohajeri1Mohammad Reza Arabestani2Manoochehr Karami3Fariba Keramat4Saba Asadollahi5Amir Khodavirdipour6Mohammad Yousef Alikhani7Department of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Kermanshah University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Epidemiology, School of Public Health, Hamadan University of Medical SciencesDepartment of Infectious Diseases, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Kermanshah University of Medical SciencesDivision of Human Genetics, Department of Anatomy, St. John’s HospitalDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesAbstract Background Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. Forty-three clinical and 26 environmental isolates of the MDR A. baumannii were collected and recognized via API 20NE. Antibiotic resistance of the isolates was assessed by the disk diffusion method, and the biofilm formation test was done by the microtiter plate method. Pulsed Field Gel Electrophoresis (PFGE) was used to assess the genomic features of the bacterial isolates. Results The resistance rate of clinical and environmental isolates against antibiotics were from 95 to 100%. The difference in antibiotic resistance rates between clinical and environmental isolates was not statistically significant (p > 0.05). Biofilm production capabilities revealed that 31 (44.9%), and 30 (43.5%) isolates had strong and moderate biofilm producer activity, respectively. PFGE typing exhibited eight different clusters (A, B, C, D, E, F, G, and H) with two significant clusters included A and G with 21 (30.4%) and 16 (23.2%) members respectively, which comprises up to 53.6% of all isolates. There was no relationship between biofilm formation and antibiotic resistance patterns with PFGE pulsotypes. Conclusions The results show that there is a close relationship between environmental and clinical isolates of A. baumannii. Cross-contamination is also very important that occurs through daily clinical activities between environmental and clinical isolates. Therefore, in order to reduce the clonal contamination of MDR A. baumannii environmental and clinical isolates, it is necessary to use strict infection control strategies.http://link.springer.com/article/10.1186/s12866-020-01792-wA.baumanniiMDRBiofilm formationAntibiotic resistancePFGE |
| spellingShingle | Ali Mohammadi Bardbari Parviz Mohajeri Mohammad Reza Arabestani Manoochehr Karami Fariba Keramat Saba Asadollahi Amir Khodavirdipour Mohammad Yousef Alikhani Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis BMC Microbiology A.baumannii MDR Biofilm formation Antibiotic resistance PFGE |
| title | Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis |
| title_full | Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis |
| title_fullStr | Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis |
| title_full_unstemmed | Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis |
| title_short | Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis |
| title_sort | molecular typing of multi drug resistant acinetobacter baumannii isolates from clinical and environmental specimens in three iranian hospitals by pulsed field gel electrophoresis |
| topic | A.baumannii MDR Biofilm formation Antibiotic resistance PFGE |
| url | http://link.springer.com/article/10.1186/s12866-020-01792-w |
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