Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi
An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2006-11-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002 |
| _version_ | 1828835030521085952 |
|---|---|
| author | Terezinha de Jesus Faria Rafael Sottero Ferreira Lidiane Yassumoto José Roberto Pinto de Souza Noemia Kazue Ishikawa Aneli de Melo Barbosa |
| author_facet | Terezinha de Jesus Faria Rafael Sottero Ferreira Lidiane Yassumoto José Roberto Pinto de Souza Noemia Kazue Ishikawa Aneli de Melo Barbosa |
| author_sort | Terezinha de Jesus Faria |
| collection | DOAJ |
| description | An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.<br>O óleo essencial resultante da destilação por arraste a vapor das partes aéreas de Ocimum gratissimum e o extrato etanólico obtido do resíduo da destilação foram avaliados quanto à atividade antifúngica, utilizando-se o método de difusão em agar. O óleo essencial inibiu o crescimento de todos os fungos testados, incluindo os fitopatogênicos Botryosphaeria rhodina e duas espécies de Alternaria sp, enquanto que o extrato do resíduo da destilação não apresentou atividade. O óleo essencial foi, então, submetido ao método de bioautografia em TLC para detecção do composto ativo. O componente ativo foi isolado e identificado através da análise por cromatografia gasosa acoplada à espectrometria de massas como o eugenol, constituinte majoritário do óleo estudado. Ensaios de atividade antifúngica revelaram a atividade do eugenol contra Alternaria isolada de tomate (A1) e Penicillium chrysogenum. As concentrações inibitórias mínimas foram 0,16 mg/disco e 0,31 mg/disco para Arternaria sp (A1) e P. chrysogenum, respectivamente. |
| first_indexed | 2024-12-12T17:53:02Z |
| format | Article |
| id | doaj.art-683a593f5027442eaec55648a082b037 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-12T17:53:02Z |
| publishDate | 2006-11-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-683a593f5027442eaec55648a082b0372022-12-22T00:16:47ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242006-11-0149686787110.1590/S1516-89132006000700002Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungiTerezinha de Jesus FariaRafael Sottero FerreiraLidiane YassumotoJosé Roberto Pinto de SouzaNoemia Kazue IshikawaAneli de Melo BarbosaAn investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.<br>O óleo essencial resultante da destilação por arraste a vapor das partes aéreas de Ocimum gratissimum e o extrato etanólico obtido do resíduo da destilação foram avaliados quanto à atividade antifúngica, utilizando-se o método de difusão em agar. O óleo essencial inibiu o crescimento de todos os fungos testados, incluindo os fitopatogênicos Botryosphaeria rhodina e duas espécies de Alternaria sp, enquanto que o extrato do resíduo da destilação não apresentou atividade. O óleo essencial foi, então, submetido ao método de bioautografia em TLC para detecção do composto ativo. O componente ativo foi isolado e identificado através da análise por cromatografia gasosa acoplada à espectrometria de massas como o eugenol, constituinte majoritário do óleo estudado. Ensaios de atividade antifúngica revelaram a atividade do eugenol contra Alternaria isolada de tomate (A1) e Penicillium chrysogenum. As concentrações inibitórias mínimas foram 0,16 mg/disco e 0,31 mg/disco para Arternaria sp (A1) e P. chrysogenum, respectivamente.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002Ocimum gratissimumantifungal activityeugenolphytopathogenic fungi |
| spellingShingle | Terezinha de Jesus Faria Rafael Sottero Ferreira Lidiane Yassumoto José Roberto Pinto de Souza Noemia Kazue Ishikawa Aneli de Melo Barbosa Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi Brazilian Archives of Biology and Technology Ocimum gratissimum antifungal activity eugenol phytopathogenic fungi |
| title | Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi |
| title_full | Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi |
| title_fullStr | Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi |
| title_full_unstemmed | Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi |
| title_short | Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi |
| title_sort | antifungal activity of essential oil isolated from ocimum gratissimum l eugenol chemotype against phytopathogenic fungi |
| topic | Ocimum gratissimum antifungal activity eugenol phytopathogenic fungi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002 |
| work_keys_str_mv | AT terezinhadejesusfaria antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi AT rafaelsotteroferreira antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi AT lidianeyassumoto antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi AT joserobertopintodesouza antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi AT noemiakazueishikawa antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi AT anelidemelobarbosa antifungalactivityofessentialoilisolatedfromocimumgratissimumleugenolchemotypeagainstphytopathogenicfungi |