Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health?
The main objective of this study was to assess the effects of 4 g·kg-1dietary MOS inclusion in soybean oil (SBO) and fish oil (FO) based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promot...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00397/full |
| _version_ | 1818279328446676992 |
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| author | Silvia eTorrecillas Daniel eMontero Maria José eCaballero Karin ePittman Aurora eCampo Marco eCustodio John eSweetman Marisol eIzquierdo |
| author_facet | Silvia eTorrecillas Daniel eMontero Maria José eCaballero Karin ePittman Aurora eCampo Marco eCustodio John eSweetman Marisol eIzquierdo |
| author_sort | Silvia eTorrecillas |
| collection | DOAJ |
| description | The main objective of this study was to assess the effects of 4 g·kg-1dietary MOS inclusion in soybean oil (SBO) and fish oil (FO) based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promoted growth. The intestinal somatic index was not affected, however SBO reduced the intestinal fold length, while dietary MOS increased it. The dietary oil source fed produced changes on the posterior intestine fatty acid profiles irrespective of MOS dietary supplementation. SBO down-regulated the gene expression of TCRβ, COX2, IL-1β, TNFα, IL-8, IL-6, IL-10, TGFβ and Ig and up-regulated MHCII. MOS up-regulated the expression of MHCI, CD4, COX2, TNFα and Ig when included in FO based diets. However, there was a minor up-regulating effect on these genes when MOS was supplemented in the SBO based diet. Both dietary oil sources and MOS affected mean mucous cell areas within the posterior gut, however the addition of MOS to a SBO diet increased the mucous cell size over the values shown in FO fed fish. Dietary SBO also trends to reduce mucous cell density in the anterior gut relative to FO, suggesting a lower overall mucosal secretion. There are no effects of dietary oil or MOS in the skin mucosal patterns. Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut GALT-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth. However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis. This denotes the importance of a balanced dietary n3/n6 ratio for an appropriate GALT-immune response against MOS in European sea bass. |
| first_indexed | 2024-12-12T23:31:35Z |
| format | Article |
| id | doaj.art-683d571f7e454e2bb77335bb4c3eb682 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T23:31:35Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-683d571f7e454e2bb77335bb4c3eb6822022-12-22T00:07:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-08-01610.3389/fimmu.2015.00397147777Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health?Silvia eTorrecillas0Daniel eMontero1Maria José eCaballero2Karin ePittman3Aurora eCampo4Marco eCustodio5John eSweetman6Marisol eIzquierdo7University of Las Palmas de Gran Canaria. Research Group in Aquaculture (GIA)University of Las Palmas de Gran Canaria. Research Group in Aquaculture (GIA)University of Las Palmas de Gran Canaria. Research Group in Aquaculture (GIA)University of BergenUniversity of BergenUniversity of BergenAlltech AquaUniversity of Las Palmas de Gran Canaria. Research Group in Aquaculture (GIA)The main objective of this study was to assess the effects of 4 g·kg-1dietary MOS inclusion in soybean oil (SBO) and fish oil (FO) based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promoted growth. The intestinal somatic index was not affected, however SBO reduced the intestinal fold length, while dietary MOS increased it. The dietary oil source fed produced changes on the posterior intestine fatty acid profiles irrespective of MOS dietary supplementation. SBO down-regulated the gene expression of TCRβ, COX2, IL-1β, TNFα, IL-8, IL-6, IL-10, TGFβ and Ig and up-regulated MHCII. MOS up-regulated the expression of MHCI, CD4, COX2, TNFα and Ig when included in FO based diets. However, there was a minor up-regulating effect on these genes when MOS was supplemented in the SBO based diet. Both dietary oil sources and MOS affected mean mucous cell areas within the posterior gut, however the addition of MOS to a SBO diet increased the mucous cell size over the values shown in FO fed fish. Dietary SBO also trends to reduce mucous cell density in the anterior gut relative to FO, suggesting a lower overall mucosal secretion. There are no effects of dietary oil or MOS in the skin mucosal patterns. Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut GALT-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth. However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis. This denotes the importance of a balanced dietary n3/n6 ratio for an appropriate GALT-immune response against MOS in European sea bass.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00397/fullSoybean OilGALTMannan oligosaccharidesEuropean sea bassmucus production. |
| spellingShingle | Silvia eTorrecillas Daniel eMontero Maria José eCaballero Karin ePittman Aurora eCampo Marco eCustodio John eSweetman Marisol eIzquierdo Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? Frontiers in Immunology Soybean Oil GALT Mannan oligosaccharides European sea bass mucus production. |
| title | Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? |
| title_full | Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? |
| title_fullStr | Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? |
| title_full_unstemmed | Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? |
| title_short | Dietary mannan oligosaccharides: Counteracting the side effects of soybean oil inclusion on European sea bass (Dicentrarchus labrax) gut health? |
| title_sort | dietary mannan oligosaccharides counteracting the side effects of soybean oil inclusion on european sea bass dicentrarchus labrax gut health |
| topic | Soybean Oil GALT Mannan oligosaccharides European sea bass mucus production. |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00397/full |
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