TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways
Background: Transforming growth factor β3 (TGFβ3) is a member of TGFβ superfamily. Studies have shown that TGFβ is involved in adipose tissue biology. Whether TGFβ3 is a crucial regulator in adipogenic differentiation of bovine preadipocytes remains unclear. The main objective of this study was to i...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S071734582200046X |
| _version_ | 1797959595009769472 |
|---|---|
| author | Liang Yang Hongzhuang Wang Weiguang Hao Tingting Li Haoyuan Fang Hui Bai Peishi Yan Shengjuan Wei |
| author_facet | Liang Yang Hongzhuang Wang Weiguang Hao Tingting Li Haoyuan Fang Hui Bai Peishi Yan Shengjuan Wei |
| author_sort | Liang Yang |
| collection | DOAJ |
| description | Background: Transforming growth factor β3 (TGFβ3) is a member of TGFβ superfamily. Studies have shown that TGFβ is involved in adipose tissue biology. Whether TGFβ3 is a crucial regulator in adipogenic differentiation of bovine preadipocytes remains unclear. The main objective of this study was to investigate the effect of TGFβ3 on the adipogenic differentiation of bovine preadipocytes as well as the underlying mechanisms. Results: Primary subcutaneous preadipocytes derived from bovine subcutaneous adipose tissue were isolated and used as a cellular model. The results showed that TGFβ3 significantly promoted the proliferation of bovine preadipocytes and markedly decreased the lipid accumulation and expression levels of key adipogenic marker genes (PPARγ, c/EBPα, and FABP4) during adipocyte differentiation. In addition, TGFβ3 significantly increased the phosphorylation levels of Smad2, Smad3, JNK, and p38 in bovine preadipocytes, which could be eliminated by SB525334 (a TGFβRI inhibitor). Further studies showed that Smad3 and p38 mediated the inhibitory effect of TGFβ3 on differentiation, whereas Smad2 attenuated the anti-adipogenic effect of TGFβ3. Conclusions: To sum up, our findings indicate that TGFβ3 is an important regulator of adipogenesis in bovine. TGFβ3 promotes the proliferation and regulates the differentiation of bovine preadipocytes through Smad2/3 and p38 signaling pathways.How to cite: Yang L, Wang H, Hao W, et al. TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways. Electron J Biotechnol 2022;61. https://doi.org/10.1016/j.ejbt.2022.11.001. |
| first_indexed | 2024-04-11T00:33:49Z |
| format | Article |
| id | doaj.art-683dade898ef489d9f9ccb770c4076b8 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-11T00:33:49Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-683dade898ef489d9f9ccb770c4076b82023-01-07T04:16:33ZengElsevierElectronic Journal of Biotechnology0717-34582023-01-01613344TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathwaysLiang Yang0Hongzhuang Wang1Weiguang Hao2Tingting Li3Haoyuan Fang4Hui Bai5Peishi Yan6Shengjuan Wei7College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaCorresponding author.; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaBackground: Transforming growth factor β3 (TGFβ3) is a member of TGFβ superfamily. Studies have shown that TGFβ is involved in adipose tissue biology. Whether TGFβ3 is a crucial regulator in adipogenic differentiation of bovine preadipocytes remains unclear. The main objective of this study was to investigate the effect of TGFβ3 on the adipogenic differentiation of bovine preadipocytes as well as the underlying mechanisms. Results: Primary subcutaneous preadipocytes derived from bovine subcutaneous adipose tissue were isolated and used as a cellular model. The results showed that TGFβ3 significantly promoted the proliferation of bovine preadipocytes and markedly decreased the lipid accumulation and expression levels of key adipogenic marker genes (PPARγ, c/EBPα, and FABP4) during adipocyte differentiation. In addition, TGFβ3 significantly increased the phosphorylation levels of Smad2, Smad3, JNK, and p38 in bovine preadipocytes, which could be eliminated by SB525334 (a TGFβRI inhibitor). Further studies showed that Smad3 and p38 mediated the inhibitory effect of TGFβ3 on differentiation, whereas Smad2 attenuated the anti-adipogenic effect of TGFβ3. Conclusions: To sum up, our findings indicate that TGFβ3 is an important regulator of adipogenesis in bovine. TGFβ3 promotes the proliferation and regulates the differentiation of bovine preadipocytes through Smad2/3 and p38 signaling pathways.How to cite: Yang L, Wang H, Hao W, et al. TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways. Electron J Biotechnol 2022;61. https://doi.org/10.1016/j.ejbt.2022.11.001.http://www.sciencedirect.com/science/article/pii/S071734582200046XAdipocyteAdipogenesis regulationAdipogenesisBovineDifferentiationMAPK |
| spellingShingle | Liang Yang Hongzhuang Wang Weiguang Hao Tingting Li Haoyuan Fang Hui Bai Peishi Yan Shengjuan Wei TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways Electronic Journal of Biotechnology Adipocyte Adipogenesis regulation Adipogenesis Bovine Differentiation MAPK |
| title | TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways |
| title_full | TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways |
| title_fullStr | TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways |
| title_full_unstemmed | TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways |
| title_short | TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways |
| title_sort | tgfβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical smad and atypical mapk signaling pathways |
| topic | Adipocyte Adipogenesis regulation Adipogenesis Bovine Differentiation MAPK |
| url | http://www.sciencedirect.com/science/article/pii/S071734582200046X |
| work_keys_str_mv | AT liangyang tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT hongzhuangwang tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT weiguanghao tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT tingtingli tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT haoyuanfang tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT huibai tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT peishiyan tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways AT shengjuanwei tgfb3regulatesadipogenesisofbovinesubcutaneouspreadipocytesviatypicalsmadandatypicalmapksignalingpathways |