Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats
The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/11/8/7879/ |
| _version_ | 1798034648349016064 |
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| author | W. Russ Algar Igor L. Medintz Guoliang Tao Robert C. Triulzi Samantha Spindel Travis Jennings Kim E. Sapsford |
| author_facet | W. Russ Algar Igor L. Medintz Guoliang Tao Robert C. Triulzi Samantha Spindel Travis Jennings Kim E. Sapsford |
| author_sort | W. Russ Algar |
| collection | DOAJ |
| description | The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took |
| first_indexed | 2024-04-11T20:47:07Z |
| format | Article |
| id | doaj.art-68446fc695d34311b5974dddc689c2e5 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:47:07Z |
| publishDate | 2011-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-68446fc695d34311b5974dddc689c2e52022-12-22T04:04:00ZengMDPI AGSensors1424-82202011-08-011187879789110.3390/s110807879Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate FormatsW. Russ AlgarIgor L. MedintzGuoliang TaoRobert C. TriulziSamantha SpindelTravis JenningsKim E. SapsfordThe simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that tookhttp://www.mdpi.com/1424-8220/11/8/7879/quantum dot (QD)nanocrystal (NC)semiconductorbioconjugationsensormultipleximmunoassaysulfhydryl chemistry |
| spellingShingle | W. Russ Algar Igor L. Medintz Guoliang Tao Robert C. Triulzi Samantha Spindel Travis Jennings Kim E. Sapsford Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats Sensors quantum dot (QD) nanocrystal (NC) semiconductor bioconjugation sensor multiplex immunoassay sulfhydryl chemistry |
| title | Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats |
| title_full | Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats |
| title_fullStr | Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats |
| title_full_unstemmed | Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats |
| title_short | Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats |
| title_sort | optimizing two color semiconductor nanocrystal immunoassays in single well microtiter plate formats |
| topic | quantum dot (QD) nanocrystal (NC) semiconductor bioconjugation sensor multiplex immunoassay sulfhydryl chemistry |
| url | http://www.mdpi.com/1424-8220/11/8/7879/ |
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