Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry...

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Bibliographic Details
Main Authors: W. Russ Algar, Igor L. Medintz, Guoliang Tao, Robert C. Triulzi, Samantha Spindel, Travis Jennings, Kim E. Sapsford
Format: Article
Language:English
Published: MDPI AG 2011-08-01
Series:Sensors
Subjects:
quantum dot (QD)
nanocrystal (NC)
semiconductor
bioconjugation
sensor
multiplex
immunoassay
sulfhydryl chemistry
Online Access:http://www.mdpi.com/1424-8220/11/8/7879/

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