Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
Ochratoxin A (OTA) produced by mycotoxigenic fungi (<i>Aspergillus</i> and <i>Penicillium</i> spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid prolife...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/6/337 |
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| author | Paloma Patricia Casas-Junco Josué Raymundo Solís-Pacheco Juan Arturo Ragazzo-Sánchez Blanca Rosa Aguilar-Uscanga Pedro Ulises Bautista-Rosales Montserrat Calderón-Santoyo |
| author_facet | Paloma Patricia Casas-Junco Josué Raymundo Solís-Pacheco Juan Arturo Ragazzo-Sánchez Blanca Rosa Aguilar-Uscanga Pedro Ulises Bautista-Rosales Montserrat Calderón-Santoyo |
| author_sort | Paloma Patricia Casas-Junco |
| collection | DOAJ |
| description | Ochratoxin A (OTA) produced by mycotoxigenic fungi (<i>Aspergillus</i> and <i>Penicillium</i> spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted coffee samples were artificially inoculated with <i>A. westerdijikiae, A. steynii, A. versicolor</i>, and <i>A. niger</i>, and incubated at 27 °C over 21 days for OTA production. Samples were cold plasma treated at 30 W input power and 850 V output voltage with helium at 1.5 L/min flow. OTA production in coffee was analyzed by high performance liquid chromatography coupled to a mass spectrometer (HPLC-MS). After 6 min of treatment with cold plasma, fungi were completely inhibited (4 log reduction). Cold plasma reduces 50% of OTA content after 30 min of treatment. Toxicity was estimated for extracts of artificially contaminated roasted coffee samples using the brine shrimp (<i>Artemia salina</i>) lethality assay. Toxicity for untreated roasted coffee was shown to be “toxic”, while toxicity for cold plasma treated coffee was reduced to “slightly toxic”. These results suggested that cold plasma may be considered as an alternative method for the degradation and reduction of toxin production by mycotoxigenic fungi in the processing of foods and feedstuffs. |
| first_indexed | 2024-04-11T20:50:09Z |
| format | Article |
| id | doaj.art-68465c646df04c33a13bb0402940c315 |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T20:50:09Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-68465c646df04c33a13bb0402940c3152022-12-22T04:03:52ZengMDPI AGToxins2072-66512019-06-0111633710.3390/toxins11060337toxins11060337Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted CoffeePaloma Patricia Casas-Junco0Josué Raymundo Solís-Pacheco1Juan Arturo Ragazzo-Sánchez2Blanca Rosa Aguilar-Uscanga3Pedro Ulises Bautista-Rosales4Montserrat Calderón-Santoyo5Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, MexicoLaboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Boulevard Marcelino García Barragán #1421, Col. Olímpica, C.P. 44430 Guadalajara, Jalisco, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, MexicoLaboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Boulevard Marcelino García Barragán #1421, Col. Olímpica, C.P. 44430 Guadalajara, Jalisco, MexicoCentro de Tecnología de Alimentos, Universidad Autónoma de Nayarit, Ciudad de la Cultura “Amado Nervo”, C.P. 63155 Tepic, Nayarit, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit, MexicoOchratoxin A (OTA) produced by mycotoxigenic fungi (<i>Aspergillus</i> and <i>Penicillium</i> spp.) is an extremely toxic and carcinogenic metabolite. The use of cold plasma to inhibit toxin-producing microorganisms in coffee could be an important alternative to avoid proliferation of mycotoxigenic fungi. Roasted coffee samples were artificially inoculated with <i>A. westerdijikiae, A. steynii, A. versicolor</i>, and <i>A. niger</i>, and incubated at 27 °C over 21 days for OTA production. Samples were cold plasma treated at 30 W input power and 850 V output voltage with helium at 1.5 L/min flow. OTA production in coffee was analyzed by high performance liquid chromatography coupled to a mass spectrometer (HPLC-MS). After 6 min of treatment with cold plasma, fungi were completely inhibited (4 log reduction). Cold plasma reduces 50% of OTA content after 30 min of treatment. Toxicity was estimated for extracts of artificially contaminated roasted coffee samples using the brine shrimp (<i>Artemia salina</i>) lethality assay. Toxicity for untreated roasted coffee was shown to be “toxic”, while toxicity for cold plasma treated coffee was reduced to “slightly toxic”. These results suggested that cold plasma may be considered as an alternative method for the degradation and reduction of toxin production by mycotoxigenic fungi in the processing of foods and feedstuffs.https://www.mdpi.com/2072-6651/11/6/337roasted coffeemycotoxigenic fungiochratoxin Acold plasmadetoxificationbrine shrimp bioassay |
| spellingShingle | Paloma Patricia Casas-Junco Josué Raymundo Solís-Pacheco Juan Arturo Ragazzo-Sánchez Blanca Rosa Aguilar-Uscanga Pedro Ulises Bautista-Rosales Montserrat Calderón-Santoyo Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee Toxins roasted coffee mycotoxigenic fungi ochratoxin A cold plasma detoxification brine shrimp bioassay |
| title | Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee |
| title_full | Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee |
| title_fullStr | Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee |
| title_full_unstemmed | Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee |
| title_short | Cold Plasma Treatment as an Alternative for Ochratoxin A Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee |
| title_sort | cold plasma treatment as an alternative for ochratoxin a detoxification and inhibition of mycotoxigenic fungi in roasted coffee |
| topic | roasted coffee mycotoxigenic fungi ochratoxin A cold plasma detoxification brine shrimp bioassay |
| url | https://www.mdpi.com/2072-6651/11/6/337 |
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