Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin
Topical treatment of wound infections is often a challenge due to limited drug availability at the site of infection. Topical drug delivery is an attractive option for reducing systemic side effects, provided that a more selective and sustained local drug delivery is achieved. In this study, a poorl...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/11/10/527 |
| _version_ | 1811186482581340160 |
|---|---|
| author | Fiorenza Rancan Marco Contardi Jana Jurisch Ulrike Blume-Peytavi Annika Vogt Ilker S. Bayer Christoph Schaudinn |
| author_facet | Fiorenza Rancan Marco Contardi Jana Jurisch Ulrike Blume-Peytavi Annika Vogt Ilker S. Bayer Christoph Schaudinn |
| author_sort | Fiorenza Rancan |
| collection | DOAJ |
| description | Topical treatment of wound infections is often a challenge due to limited drug availability at the site of infection. Topical drug delivery is an attractive option for reducing systemic side effects, provided that a more selective and sustained local drug delivery is achieved. In this study, a poorly water-soluble antibiotic, ciprofloxacin, was loaded on polyvinylpyrrolidone (PVP)-based foils and nanofiber mats using acetic acid as a solubilizer. Drug delivery kinetics, local toxicity, and antimicrobial activity were tested on an ex vivo wound model based on full-thickness human skin. Wounds of 5 mm in diameter were created on 1.5 × 1.5 cm skin blocks and treated with the investigated materials. While nanofiber mats reached the highest amount of delivered drug after 6 h, foils rapidly achieved a maximum drug concentration and maintained it over 24 h. The treatment had no effect on the overall skin metabolic activity but influenced the wound healing process, as observed using histological analysis. Both delivery systems were efficient in preventing the growth of <i>Pseudomonas aeruginosa</i> biofilms in ex vivo human skin. Interestingly, foils loaded with 500 µg of ciprofloxacin accomplished the complete eradication of biofilm infections with 1 × 10<sup>9</sup> bacteria/wound. We conclude that antimicrobial-loaded resorbable PVP foils and nanofiber mats are promising delivery systems for the prevention or topical treatment of infected wounds. |
| first_indexed | 2024-04-11T13:46:10Z |
| format | Article |
| id | doaj.art-684733bacf63475182c4b0c2fc139190 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T13:46:10Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-684733bacf63475182c4b0c2fc1391902022-12-22T04:21:02ZengMDPI AGPharmaceutics1999-49232019-10-01111052710.3390/pharmaceutics11100527pharmaceutics11100527Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human SkinFiorenza Rancan0Marco Contardi1Jana Jurisch2Ulrike Blume-Peytavi3Annika Vogt4Ilker S. Bayer5Christoph Schaudinn6Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, GermanySmart Materials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyClinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, GermanyClinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, GermanyClinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, GermanySmart Materials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyAdvanced Light and Electron Microscopy, ZBS4, Robert Koch Institute, 13353 Berlin, GermanyTopical treatment of wound infections is often a challenge due to limited drug availability at the site of infection. Topical drug delivery is an attractive option for reducing systemic side effects, provided that a more selective and sustained local drug delivery is achieved. In this study, a poorly water-soluble antibiotic, ciprofloxacin, was loaded on polyvinylpyrrolidone (PVP)-based foils and nanofiber mats using acetic acid as a solubilizer. Drug delivery kinetics, local toxicity, and antimicrobial activity were tested on an ex vivo wound model based on full-thickness human skin. Wounds of 5 mm in diameter were created on 1.5 × 1.5 cm skin blocks and treated with the investigated materials. While nanofiber mats reached the highest amount of delivered drug after 6 h, foils rapidly achieved a maximum drug concentration and maintained it over 24 h. The treatment had no effect on the overall skin metabolic activity but influenced the wound healing process, as observed using histological analysis. Both delivery systems were efficient in preventing the growth of <i>Pseudomonas aeruginosa</i> biofilms in ex vivo human skin. Interestingly, foils loaded with 500 µg of ciprofloxacin accomplished the complete eradication of biofilm infections with 1 × 10<sup>9</sup> bacteria/wound. We conclude that antimicrobial-loaded resorbable PVP foils and nanofiber mats are promising delivery systems for the prevention or topical treatment of infected wounds.https://www.mdpi.com/1999-4923/11/10/527wound infectionbiofilmpseudomonas aeruginosaantimicrobial deliverypolyvinylpyrrolidonenanofibers |
| spellingShingle | Fiorenza Rancan Marco Contardi Jana Jurisch Ulrike Blume-Peytavi Annika Vogt Ilker S. Bayer Christoph Schaudinn Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin Pharmaceutics wound infection biofilm pseudomonas aeruginosa antimicrobial delivery polyvinylpyrrolidone nanofibers |
| title | Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin |
| title_full | Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin |
| title_fullStr | Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin |
| title_full_unstemmed | Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin |
| title_short | Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin |
| title_sort | evaluation of drug delivery and efficacy of ciprofloxacin loaded povidone foils and nanofiber mats in a wound infection model based on ex vivo human skin |
| topic | wound infection biofilm pseudomonas aeruginosa antimicrobial delivery polyvinylpyrrolidone nanofibers |
| url | https://www.mdpi.com/1999-4923/11/10/527 |
| work_keys_str_mv | AT fiorenzarancan evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT marcocontardi evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT janajurisch evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT ulrikeblumepeytavi evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT annikavogt evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT ilkersbayer evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin AT christophschaudinn evaluationofdrugdeliveryandefficacyofciprofloxacinloadedpovidonefoilsandnanofibermatsinawoundinfectionmodelbasedonexvivohumanskin |