Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell
Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of <...
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/7/1411 |
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| author | Kazunari Ozasa Hyunwoong Kang Simon Song Shota Kato Tomoko Shinomura Mizuo Maeda |
| author_facet | Kazunari Ozasa Hyunwoong Kang Simon Song Shota Kato Tomoko Shinomura Mizuo Maeda |
| author_sort | Kazunari Ozasa |
| collection | DOAJ |
| description | Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of <i>Euglena gracilis</i> using vertically placed microchambers containing a single cell. A temporal change in gravitaxis and cell multiplication was observed after transferring the cells to fresh culture medium for 9 days. We performed 29 individual experiments with 2.5 mm × 2.5 mm × 0.1 mm square microchambers and found that the cells showed positive, negative, and moderate gravitaxis in 8, 7, and 14 cases, respectively, after transferring to fresh culture medium. A common trend was observed for the temporal change in gravitaxis for the eight initially positive gravitaxis cases. The cells with initially positive gravitaxis showed a higher rate of cell multiplication than those with initially negative gravitaxis. We also discussed the gravitaxis mechanism of <i>E. gracilis</i> from the observed trend of gravitaxis change and swimming traces. In addition, bioconvection in a larger and thicker chamber was investigated at a millimeter scale and visualized. |
| first_indexed | 2024-03-10T09:27:33Z |
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| id | doaj.art-6848b04335fd4b1f89a0f74f69c006f3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T09:27:33Z |
| publishDate | 2021-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-6848b04335fd4b1f89a0f74f69c006f32023-11-22T04:43:47ZengMDPI AGPlants2223-77472021-07-01107141110.3390/plants10071411Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single CellKazunari Ozasa0Hyunwoong Kang1Simon Song2Shota Kato3Tomoko Shinomura4Mizuo Maeda5Bioengineering Laboratory, Cluster for Pioneering Research, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, JapanDepartment of Mechanical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Mechanical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaCenter for Bioscience Research and Education, Utsunomiya University, Mine 350, Utsunomiya, Tochigi 321-8505, JapanPlant Molecular and Cellular Biology Laboratory, Department of Biosciences, School of Science and Engineering, Teikyo University, 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551, JapanBioengineering Laboratory, Cluster for Pioneering Research, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, JapanGravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of <i>Euglena gracilis</i> using vertically placed microchambers containing a single cell. A temporal change in gravitaxis and cell multiplication was observed after transferring the cells to fresh culture medium for 9 days. We performed 29 individual experiments with 2.5 mm × 2.5 mm × 0.1 mm square microchambers and found that the cells showed positive, negative, and moderate gravitaxis in 8, 7, and 14 cases, respectively, after transferring to fresh culture medium. A common trend was observed for the temporal change in gravitaxis for the eight initially positive gravitaxis cases. The cells with initially positive gravitaxis showed a higher rate of cell multiplication than those with initially negative gravitaxis. We also discussed the gravitaxis mechanism of <i>E. gracilis</i> from the observed trend of gravitaxis change and swimming traces. In addition, bioconvection in a larger and thicker chamber was investigated at a millimeter scale and visualized.https://www.mdpi.com/2223-7747/10/7/1411positive gravitaxismicrofluidic devicescell metabolismswimming tracesbioconvection |
| spellingShingle | Kazunari Ozasa Hyunwoong Kang Simon Song Shota Kato Tomoko Shinomura Mizuo Maeda Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell Plants positive gravitaxis microfluidic devices cell metabolism swimming traces bioconvection |
| title | Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell |
| title_full | Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell |
| title_fullStr | Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell |
| title_full_unstemmed | Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell |
| title_short | Temporal Evolution of the Gravitaxis of <i>Euglena gracilis</i> from a Single Cell |
| title_sort | temporal evolution of the gravitaxis of i euglena gracilis i from a single cell |
| topic | positive gravitaxis microfluidic devices cell metabolism swimming traces bioconvection |
| url | https://www.mdpi.com/2223-7747/10/7/1411 |
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