Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway
MicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after <i>Rhizoctonia solani</i> (<...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3653 |
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| author | Tao Feng Zhao-Yang Zhang Peng Gao Zhi-Ming Feng Shi-Min Zuo Shou-Qiang Ouyang |
| author_facet | Tao Feng Zhao-Yang Zhang Peng Gao Zhi-Ming Feng Shi-Min Zuo Shou-Qiang Ouyang |
| author_sort | Tao Feng |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after <i>Rhizoctonia solani</i> (<i>R. solani</i>) infection. In order to clarify the function of Osa-miR444b.2 responding to <i>R. solani</i> infection in rice, transgenic lines over-expressing and knocking out Osa-miR444b.2 were generated in the background of susceptible cultivar Xu3 and resistant cultivar YSBR1, respectively. Over-expressing Osa-miR444b.2 resulted in compromised resistance to <i>R. solani</i>. In contrast, the knocking out Osa-miR444b.2 lines exhibited improved resistance to <i>R. solani</i>. Furthermore, knocking out Osa-miR444b.2 resulted in increased height, tillers, smaller panicle, and decreased 1000-grain weight and primary branches. However, the transgenic lines over-expressing Osa-miR444b.2 showed decreased primary branches and tillers, but increased panicle length. These results indicated that Osa-miR444b.2 was also involved in regulating the agronomic traits in rice. The RNA-seq assay revealed that Osa-miR444b.2 mainly regulated the resistance to rice sheath blight disease by affecting the expression of plant hormone signaling pathways-related genes such as ET and IAA, and transcription factors such as WRKYs and F-boxes. Together, our results suggest that Osa-miR444b.2 negatively mediated the resistance to <i>R. solani</i> in rice, which will contribute to the cultivation of sheath blight resistant varieties. |
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| last_indexed | 2024-03-11T08:42:57Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-684a0830b7cc40f986b3455c74f9e7862023-11-16T21:03:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244365310.3390/ijms24043653Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone PathwayTao Feng0Zhao-Yang Zhang1Peng Gao2Zhi-Ming Feng3Shi-Min Zuo4Shou-Qiang Ouyang5College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaMicroRNAs (miRNAs) are a class of conserved small RNA with a length of 21–24 nucleotides in eukaryotes, which are involved in development and defense responses against biotic and abiotic stresses. By RNA-seq, Osa-miR444b.2 was identified to be induced after <i>Rhizoctonia solani</i> (<i>R. solani</i>) infection. In order to clarify the function of Osa-miR444b.2 responding to <i>R. solani</i> infection in rice, transgenic lines over-expressing and knocking out Osa-miR444b.2 were generated in the background of susceptible cultivar Xu3 and resistant cultivar YSBR1, respectively. Over-expressing Osa-miR444b.2 resulted in compromised resistance to <i>R. solani</i>. In contrast, the knocking out Osa-miR444b.2 lines exhibited improved resistance to <i>R. solani</i>. Furthermore, knocking out Osa-miR444b.2 resulted in increased height, tillers, smaller panicle, and decreased 1000-grain weight and primary branches. However, the transgenic lines over-expressing Osa-miR444b.2 showed decreased primary branches and tillers, but increased panicle length. These results indicated that Osa-miR444b.2 was also involved in regulating the agronomic traits in rice. The RNA-seq assay revealed that Osa-miR444b.2 mainly regulated the resistance to rice sheath blight disease by affecting the expression of plant hormone signaling pathways-related genes such as ET and IAA, and transcription factors such as WRKYs and F-boxes. Together, our results suggest that Osa-miR444b.2 negatively mediated the resistance to <i>R. solani</i> in rice, which will contribute to the cultivation of sheath blight resistant varieties.https://www.mdpi.com/1422-0067/24/4/3653ricemicroRNAsheath blight diseaseresistanceplant hormones |
| spellingShingle | Tao Feng Zhao-Yang Zhang Peng Gao Zhi-Ming Feng Shi-Min Zuo Shou-Qiang Ouyang Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway International Journal of Molecular Sciences rice microRNA sheath blight disease resistance plant hormones |
| title | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
| title_full | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
| title_fullStr | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
| title_full_unstemmed | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
| title_short | Suppression of Rice Osa-miR444.2 Improves the Resistance to Sheath Blight in Rice Mediating through the Phytohormone Pathway |
| title_sort | suppression of rice osa mir444 2 improves the resistance to sheath blight in rice mediating through the phytohormone pathway |
| topic | rice microRNA sheath blight disease resistance plant hormones |
| url | https://www.mdpi.com/1422-0067/24/4/3653 |
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