Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module
The increased interest in <sup>89</sup>Zr-labelled immunoPET imaging probes for use in preclinical and clinical studies has led to a rising demand for the isotope. The highly penetrating 511 and 909 keV photons emitted by <sup>89</sup>Zr deliver an undesirably high radiation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/11/2626 |
| _version_ | 1797566088915976192 |
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| author | Vijay Gaja Jacqueline Cawthray Clarence R. Geyer Humphrey Fonge |
| author_facet | Vijay Gaja Jacqueline Cawthray Clarence R. Geyer Humphrey Fonge |
| author_sort | Vijay Gaja |
| collection | DOAJ |
| description | The increased interest in <sup>89</sup>Zr-labelled immunoPET imaging probes for use in preclinical and clinical studies has led to a rising demand for the isotope. The highly penetrating 511 and 909 keV photons emitted by <sup>89</sup>Zr deliver an undesirably high radiation dose, which makes it difficult to produce large amounts manually. Additionally, there is a growing demand for Good Manufacturing Practices (GMP)-grade radionuclides for clinical applications. In this study, we have adopted the commercially available TRASIS mini AllinOne (miniAiO) automated synthesis unit to achieve efficient and reproducible batches of <sup>89</sup>Zr. This automated module is used for the target dissolution and separation of <sup>89</sup>Zr from the yttrium target material. The <sup>89</sup>Zr is eluted with a very small volume of oxalic acid (1.5 mL) directly over the sterile filter into the final vial. Using this sophisticated automated purification method, we obtained satisfactory amount of <sup>89</sup>Zr in high radionuclidic and radiochemical purities in excess of 99.99%. The specific activity of three production batches were calculated and was found to be in the range of 1351–2323 MBq/µmol. ICP-MS analysis of final solutions showed impurity levels always below 1 ppm. |
| first_indexed | 2024-03-10T19:21:47Z |
| format | Article |
| id | doaj.art-684a4566cdf2438794d56b55a14d9e9f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:21:47Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-684a4566cdf2438794d56b55a14d9e9f2023-11-20T02:55:58ZengMDPI AGMolecules1420-30492020-06-012511262610.3390/molecules25112626Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO ModuleVijay Gaja0Jacqueline Cawthray1Clarence R. Geyer2Humphrey Fonge3Department of Medical Imaging, University of Saskatchewan, College of Medicine, Saskatoon, SK S7N 0W8, CanadaSaskatchewan Centre for Cyclotron Sciences, Saskatoon, SK S7N 5C4, CanadaDepartment of Pathology and Laboratory Medicine, University of Saskatchewan, College of Medicine, Saskatoon, SK S7N 5E5, CanadaDepartment of Medical Imaging, University of Saskatchewan, College of Medicine, Saskatoon, SK S7N 0W8, CanadaThe increased interest in <sup>89</sup>Zr-labelled immunoPET imaging probes for use in preclinical and clinical studies has led to a rising demand for the isotope. The highly penetrating 511 and 909 keV photons emitted by <sup>89</sup>Zr deliver an undesirably high radiation dose, which makes it difficult to produce large amounts manually. Additionally, there is a growing demand for Good Manufacturing Practices (GMP)-grade radionuclides for clinical applications. In this study, we have adopted the commercially available TRASIS mini AllinOne (miniAiO) automated synthesis unit to achieve efficient and reproducible batches of <sup>89</sup>Zr. This automated module is used for the target dissolution and separation of <sup>89</sup>Zr from the yttrium target material. The <sup>89</sup>Zr is eluted with a very small volume of oxalic acid (1.5 mL) directly over the sterile filter into the final vial. Using this sophisticated automated purification method, we obtained satisfactory amount of <sup>89</sup>Zr in high radionuclidic and radiochemical purities in excess of 99.99%. The specific activity of three production batches were calculated and was found to be in the range of 1351–2323 MBq/µmol. ICP-MS analysis of final solutions showed impurity levels always below 1 ppm.https://www.mdpi.com/1420-3049/25/11/2626Zirconium-89automationradiolabelling |
| spellingShingle | Vijay Gaja Jacqueline Cawthray Clarence R. Geyer Humphrey Fonge Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module Molecules Zirconium-89 automation radiolabelling |
| title | Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module |
| title_full | Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module |
| title_fullStr | Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module |
| title_full_unstemmed | Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module |
| title_short | Production and Semi-Automated Processing of <sup>89</sup>Zr Using a Commercially Available TRASIS MiniAiO Module |
| title_sort | production and semi automated processing of sup 89 sup zr using a commercially available trasis miniaio module |
| topic | Zirconium-89 automation radiolabelling |
| url | https://www.mdpi.com/1420-3049/25/11/2626 |
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