Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis
Background and Objective: Multiple sclerosis (MS) is a common neurological disease that increases oxidative stress and causes immune system disorders. Curcumin is the active component of turmeric with anti-inflammatory properties. This study was conducted to determine the effects of curcumin on cort...
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| Format: | Article |
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Golestan University of Medical Sciences
2022-07-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/article-1-4041-en.html |
| _version_ | 1798030254693941248 |
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| author | Laleh Mavaddatiyan Shiva Khezri Seyyed Meysam Abtahi Froushani |
| author_facet | Laleh Mavaddatiyan Shiva Khezri Seyyed Meysam Abtahi Froushani |
| author_sort | Laleh Mavaddatiyan |
| collection | DOAJ |
| description | Background and Objective: Multiple sclerosis (MS) is a common neurological disease that increases oxidative stress and causes immune system disorders. Curcumin is the active component of turmeric with anti-inflammatory properties. This study was conducted to determine the effects of curcumin on cortisol, catalase, and nerve growth factor (NGF) expression in an animal model of MS.
Methods: This experimental study was conducted on 30 female Wistar rats. Experimental autoimmune encephalomyelitis (EAE) was chosen as an experimental model of MS. The rats were divided into 3 groups of 10, including a healthy control group, an affected group, and a group treated with curcumin. The disease was induced by immunization of rats with homogenized guinea pig spinal cord and Freund's complete adjuvant. Then, the immunized animals were allocated into two equal groups. Treatment with curcumin (100 mg/kg daily) was started 12 days after the immunization when the rats showed the first symptoms of neurologic disability. The treatment was continued until day 24 post-immunization. Simultaneously, the EAE group received the medicine solvent (distilled water). Finally, the rats' weights as well as cortisol, catalase, and NGF levels were measured in the study groups.
Results: Curcumin significantly increased the level of cortisol to a level equal to that of healthy rats (P<0.05). It also significantly increased the expression of NGF and reduced the amount of catalase in the affected rats (P<0.05). The curcumin administration significantly increased the overall weight of rats with MS but had no significant effect on the spleen weight of the treated rats.
Conclusion: Curcumin can be beneficial for treating EAE by reducing the destructive effects of oxidative damage and increasing NGF. |
| first_indexed | 2024-04-11T19:37:19Z |
| format | Article |
| id | doaj.art-684c998f1a9f46dd8a3d3648212d1cb9 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-04-11T19:37:19Z |
| publishDate | 2022-07-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-684c998f1a9f46dd8a3d3648212d1cb92022-12-22T04:06:48ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802022-07-012421018Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple SclerosisLaleh Mavaddatiyan0Shiva Khezri1Seyyed Meysam Abtahi Froushani2 M.Sc in Animal Physiology, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran. Assistant Professor, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran. Associate Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Background and Objective: Multiple sclerosis (MS) is a common neurological disease that increases oxidative stress and causes immune system disorders. Curcumin is the active component of turmeric with anti-inflammatory properties. This study was conducted to determine the effects of curcumin on cortisol, catalase, and nerve growth factor (NGF) expression in an animal model of MS. Methods: This experimental study was conducted on 30 female Wistar rats. Experimental autoimmune encephalomyelitis (EAE) was chosen as an experimental model of MS. The rats were divided into 3 groups of 10, including a healthy control group, an affected group, and a group treated with curcumin. The disease was induced by immunization of rats with homogenized guinea pig spinal cord and Freund's complete adjuvant. Then, the immunized animals were allocated into two equal groups. Treatment with curcumin (100 mg/kg daily) was started 12 days after the immunization when the rats showed the first symptoms of neurologic disability. The treatment was continued until day 24 post-immunization. Simultaneously, the EAE group received the medicine solvent (distilled water). Finally, the rats' weights as well as cortisol, catalase, and NGF levels were measured in the study groups. Results: Curcumin significantly increased the level of cortisol to a level equal to that of healthy rats (P<0.05). It also significantly increased the expression of NGF and reduced the amount of catalase in the affected rats (P<0.05). The curcumin administration significantly increased the overall weight of rats with MS but had no significant effect on the spleen weight of the treated rats. Conclusion: Curcumin can be beneficial for treating EAE by reducing the destructive effects of oxidative damage and increasing NGF.http://goums.ac.ir/journal/article-1-4041-en.htmlmultiple sclerosisexperimental autoimmune encephalomyelitiscurcumincatalasenerve growth factorwistar rats |
| spellingShingle | Laleh Mavaddatiyan Shiva Khezri Seyyed Meysam Abtahi Froushani Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis مجله دانشگاه علوم پزشکی گرگان multiple sclerosis experimental autoimmune encephalomyelitis curcumin catalase nerve growth factor wistar rats |
| title | Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis |
| title_full | Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis |
| title_fullStr | Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis |
| title_full_unstemmed | Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis |
| title_short | Effect of Curcumin on Cortisol, Catalase and Nerve Growth Factor Expression Level in Animal Model of Induced Multiple Sclerosis |
| title_sort | effect of curcumin on cortisol catalase and nerve growth factor expression level in animal model of induced multiple sclerosis |
| topic | multiple sclerosis experimental autoimmune encephalomyelitis curcumin catalase nerve growth factor wistar rats |
| url | http://goums.ac.ir/journal/article-1-4041-en.html |
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