Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage
Background and Aim: Lecithin based diluent such as AndroMed, is a semen diluent made without animal components to prevent the risk of disease transmission, while glutathione (GSH) is an intracellular non-enzymatic antioxidant that prevents cell damage due to reactive oxygen species. Meanwhile, the s...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-09-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/September-2021/30.pdf |
| _version_ | 1819292258970632192 |
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| author | Frediansyah Firdaus Dian Ratnawati |
| author_facet | Frediansyah Firdaus Dian Ratnawati |
| author_sort | Frediansyah Firdaus |
| collection | DOAJ |
| description | Background and Aim: Lecithin based diluent such as AndroMed, is a semen diluent made without animal components to prevent the risk of disease transmission, while glutathione (GSH) is an intracellular non-enzymatic antioxidant that prevents cell damage due to reactive oxygen species. Meanwhile, the specific impact of AndroMed and GSH combinations on spermatozoa abnormalities has not been fully studied. Therefore, this study aims to determine the effect of using cauda epididymal plasma-2 (CEP-2) and AndroMed diluents with or without the addition of GSH on the abnormalities of sexing semen of Ongole crossbred bulls in cold storage.
Materials and Methods: This study used a factorial completely randomized design 2×2, the first factor was types of diluent and the second was with or without the addition of GSH. Observation of spermatozoa abnormalities was carried out at a storage time of 0-5 days using 297 ejaculations of liquid semen, with 100 spermatozoa observed per smear of each ejaculate. Furthermore, the data were analyzed using a two-way analysis of variance and the significant threshold (p-value) for statistical analysis was set at <0.05.
Results: The results showed that there was a significant difference (p<0.05) between AndroMed and CEP-2 in minimizing the abnormalities of upper layer spermatozoa (X), with parameters DH and AD on day 0, damage of spermatozoa (DMR) on days 1-5, and dag-like defect (DLD) on day 5. Furthermore, spermatozoa abnormalities in the lower layer (Y) showed a significant difference between diluents in the parameters of AD on day 1, DMR on days 0-5, and DLD on days 1-5. The significant difference between with or without the addition of GSH in the X sperm was observed in the DH parameters on day 0 and DMR on 5, while there was no significant difference in the Y sperm.
Conclusion: Based on the results, AndroMed has the potential to minimize spermatozoa abnormalities compared to CEP-2 diluent in sexed liquid semen. Therefore, AndroMed diluents with or without the addition of 1 mM GSH have no significant effect on spermatozoa abnormalities. |
| first_indexed | 2024-12-24T03:51:41Z |
| format | Article |
| id | doaj.art-685160cc9c6c4bf0ba3aa0b3e2995796 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-24T03:51:41Z |
| publishDate | 2021-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-685160cc9c6c4bf0ba3aa0b3e29957962022-12-21T17:16:34ZengVeterinary WorldVeterinary World0972-89882231-09162021-09-011492543254810.14202/vetworld.2021.2543-2548Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storageFrediansyah Firdaus0https://orcid.org/0000-0002-6810-8769Dian Ratnawati1https://orcid.org/0000-0003-1516-9549Department of Animal Physiology and Reproduction, Beef Cattle Research Institute, Pasuruan East Java 67184, Indonesia.Department of Animal Physiology and Reproduction, Beef Cattle Research Institute, Pasuruan East Java 67184, Indonesia.Background and Aim: Lecithin based diluent such as AndroMed, is a semen diluent made without animal components to prevent the risk of disease transmission, while glutathione (GSH) is an intracellular non-enzymatic antioxidant that prevents cell damage due to reactive oxygen species. Meanwhile, the specific impact of AndroMed and GSH combinations on spermatozoa abnormalities has not been fully studied. Therefore, this study aims to determine the effect of using cauda epididymal plasma-2 (CEP-2) and AndroMed diluents with or without the addition of GSH on the abnormalities of sexing semen of Ongole crossbred bulls in cold storage. Materials and Methods: This study used a factorial completely randomized design 2×2, the first factor was types of diluent and the second was with or without the addition of GSH. Observation of spermatozoa abnormalities was carried out at a storage time of 0-5 days using 297 ejaculations of liquid semen, with 100 spermatozoa observed per smear of each ejaculate. Furthermore, the data were analyzed using a two-way analysis of variance and the significant threshold (p-value) for statistical analysis was set at <0.05. Results: The results showed that there was a significant difference (p<0.05) between AndroMed and CEP-2 in minimizing the abnormalities of upper layer spermatozoa (X), with parameters DH and AD on day 0, damage of spermatozoa (DMR) on days 1-5, and dag-like defect (DLD) on day 5. Furthermore, spermatozoa abnormalities in the lower layer (Y) showed a significant difference between diluents in the parameters of AD on day 1, DMR on days 0-5, and DLD on days 1-5. The significant difference between with or without the addition of GSH in the X sperm was observed in the DH parameters on day 0 and DMR on 5, while there was no significant difference in the Y sperm. Conclusion: Based on the results, AndroMed has the potential to minimize spermatozoa abnormalities compared to CEP-2 diluent in sexed liquid semen. Therefore, AndroMed diluents with or without the addition of 1 mM GSH have no significant effect on spermatozoa abnormalities.http://www.veterinaryworld.org/Vol.14/September-2021/30.pdflecithincauda epididymal plasma-2glutathionespermatozoa abnormality |
| spellingShingle | Frediansyah Firdaus Dian Ratnawati Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage Veterinary World lecithin cauda epididymal plasma-2 glutathione spermatozoa abnormality |
| title | Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| title_full | Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| title_fullStr | Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| title_full_unstemmed | Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| title_short | Effectiveness of cauda epididymal plasma-2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| title_sort | effectiveness of cauda epididymal plasma 2 and lecithin based diluents to minimize abnormality of sexing albumin spermatozoa during cold storage |
| topic | lecithin cauda epididymal plasma-2 glutathione spermatozoa abnormality |
| url | http://www.veterinaryworld.org/Vol.14/September-2021/30.pdf |
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