Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells
One of the most common regenerative therapies is autologous fat grafting, which frequently suffers from unexpected volume loss. One approach is to deliver adipose stem cells encapsulated in the engineered hydrogels supportive of cell survival, differentiation, and integration after transplant. We de...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2016-09-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731416670482 |
| _version_ | 1818441805605109760 |
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| author | Tracy N Clevenger Gabriel Luna Daniel Boctor Steven K Fisher Dennis O Clegg |
| author_facet | Tracy N Clevenger Gabriel Luna Daniel Boctor Steven K Fisher Dennis O Clegg |
| author_sort | Tracy N Clevenger |
| collection | DOAJ |
| description | One of the most common regenerative therapies is autologous fat grafting, which frequently suffers from unexpected volume loss. One approach is to deliver adipose stem cells encapsulated in the engineered hydrogels supportive of cell survival, differentiation, and integration after transplant. We describe an encapsulating, biomimetic poly(ethylene)-glycol hydrogel, with embedded peptides for attachment and biodegradation. Poly(ethylene)-glycol hydrogels containing an Arg–Gly–Asp attachment sequence and a matrix metalloprotease 3/10 cleavage site supported adipose stem cell survival and showed remodeling initiated by adipogenic differentiation. Arg–Gly–Asp–matrix metalloprotease 3/10 cleavage site hydrogels showed an increased number and area of lacunae or holes after adipose stem cell differentiation. Image analysis of adipose stem cells in Arg–Gly–Asp–matrix metalloprotease 3/10 cleavage site hydrogels showed larger Voronoi domains, while cell density remained unchanged. The differentiated adipocytes residing within these newly remodeled spaces express proteins and messenger RNAs indicative of adipocytic differentiation. These engineered scaffolds may provide niches for stem cell differentiation and could prove useful in soft tissue regeneration. |
| first_indexed | 2024-12-14T18:34:05Z |
| format | Article |
| id | doaj.art-68578eb8d79b4b28a3b5263e80479c01 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-14T18:34:05Z |
| publishDate | 2016-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-68578eb8d79b4b28a3b5263e80479c012022-12-21T22:51:40ZengSAGE PublishingJournal of Tissue Engineering2041-73142016-09-01710.1177/204173141667048210.1177_2041731416670482Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cellsTracy N Clevenger0Gabriel Luna1Daniel Boctor2Steven K Fisher3Dennis O Clegg4Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USACenter for Bio-Image Informatics, University of California, Santa Barbara, CA, USANeuroscience Research Institute, University of California, Santa Barbara, CA, USACenter for Bio-Image Informatics, University of California, Santa Barbara, CA, USADepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USAOne of the most common regenerative therapies is autologous fat grafting, which frequently suffers from unexpected volume loss. One approach is to deliver adipose stem cells encapsulated in the engineered hydrogels supportive of cell survival, differentiation, and integration after transplant. We describe an encapsulating, biomimetic poly(ethylene)-glycol hydrogel, with embedded peptides for attachment and biodegradation. Poly(ethylene)-glycol hydrogels containing an Arg–Gly–Asp attachment sequence and a matrix metalloprotease 3/10 cleavage site supported adipose stem cell survival and showed remodeling initiated by adipogenic differentiation. Arg–Gly–Asp–matrix metalloprotease 3/10 cleavage site hydrogels showed an increased number and area of lacunae or holes after adipose stem cell differentiation. Image analysis of adipose stem cells in Arg–Gly–Asp–matrix metalloprotease 3/10 cleavage site hydrogels showed larger Voronoi domains, while cell density remained unchanged. The differentiated adipocytes residing within these newly remodeled spaces express proteins and messenger RNAs indicative of adipocytic differentiation. These engineered scaffolds may provide niches for stem cell differentiation and could prove useful in soft tissue regeneration.https://doi.org/10.1177/2041731416670482 |
| spellingShingle | Tracy N Clevenger Gabriel Luna Daniel Boctor Steven K Fisher Dennis O Clegg Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells Journal of Tissue Engineering |
| title | Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| title_full | Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| title_fullStr | Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| title_full_unstemmed | Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| title_short | Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| title_sort | cell mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells |
| url | https://doi.org/10.1177/2041731416670482 |
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