A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria.
Cerebral Malaria (CM) is associated with a pathogenic T cell response. Mice infected by P. berghei ANKA clone 1.49 (PbA) developing CM (CM+) present an altered PBL TCR repertoire, partly due to recurrently expanded T cell clones, as compared to non-infected and CM- infected mice. To analyse the rela...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4742225?pdf=render |
| _version_ | 1811330649315868672 |
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| author | Encarnita Mariotti-Ferrandiz Hang-Phuong Pham Sophie Dulauroy Olivier Gorgette David Klatzmann Pierre-André Cazenave Sylviane Pied Adrien Six |
| author_facet | Encarnita Mariotti-Ferrandiz Hang-Phuong Pham Sophie Dulauroy Olivier Gorgette David Klatzmann Pierre-André Cazenave Sylviane Pied Adrien Six |
| author_sort | Encarnita Mariotti-Ferrandiz |
| collection | DOAJ |
| description | Cerebral Malaria (CM) is associated with a pathogenic T cell response. Mice infected by P. berghei ANKA clone 1.49 (PbA) developing CM (CM+) present an altered PBL TCR repertoire, partly due to recurrently expanded T cell clones, as compared to non-infected and CM- infected mice. To analyse the relationship between repertoire alteration and CM, we performed a kinetic analysis of the TRBV repertoire during the course of the infection until CM-related death in PbA-infected mice. The repertoires of PBL, splenocytes and brain lymphocytes were compared between infected and non-infected mice using a high-throughput CDR3 spectratyping method. We observed a modification of the whole TCR repertoire in the spleen and blood of infected mice, from the fifth and the sixth day post-infection, respectively, while only three TRBV were significantly perturbed in the brain of infected mice. Using multivariate analysis and statistical modelling, we identified a unique TCRβ signature discriminating CM+ from CTR mice, enriched during the course of the infection in the spleen and the blood and predicting CM onset. These results highlight a dynamic modification and compartmentalization of the TCR diversity during the course of PbA infection, and provide a novel method to identify disease-associated TCRβ signature as diagnostic and prognostic biomarkers. |
| first_indexed | 2024-04-13T16:06:51Z |
| format | Article |
| id | doaj.art-685e1d37379643249d5f328d5f7f28a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T16:06:51Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-685e1d37379643249d5f328d5f7f28a82022-12-22T02:40:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e014787110.1371/journal.pone.0147871A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria.Encarnita Mariotti-FerrandizHang-Phuong PhamSophie DulauroyOlivier GorgetteDavid KlatzmannPierre-André CazenaveSylviane PiedAdrien SixCerebral Malaria (CM) is associated with a pathogenic T cell response. Mice infected by P. berghei ANKA clone 1.49 (PbA) developing CM (CM+) present an altered PBL TCR repertoire, partly due to recurrently expanded T cell clones, as compared to non-infected and CM- infected mice. To analyse the relationship between repertoire alteration and CM, we performed a kinetic analysis of the TRBV repertoire during the course of the infection until CM-related death in PbA-infected mice. The repertoires of PBL, splenocytes and brain lymphocytes were compared between infected and non-infected mice using a high-throughput CDR3 spectratyping method. We observed a modification of the whole TCR repertoire in the spleen and blood of infected mice, from the fifth and the sixth day post-infection, respectively, while only three TRBV were significantly perturbed in the brain of infected mice. Using multivariate analysis and statistical modelling, we identified a unique TCRβ signature discriminating CM+ from CTR mice, enriched during the course of the infection in the spleen and the blood and predicting CM onset. These results highlight a dynamic modification and compartmentalization of the TCR diversity during the course of PbA infection, and provide a novel method to identify disease-associated TCRβ signature as diagnostic and prognostic biomarkers.http://europepmc.org/articles/PMC4742225?pdf=render |
| spellingShingle | Encarnita Mariotti-Ferrandiz Hang-Phuong Pham Sophie Dulauroy Olivier Gorgette David Klatzmann Pierre-André Cazenave Sylviane Pied Adrien Six A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. PLoS ONE |
| title | A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. |
| title_full | A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. |
| title_fullStr | A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. |
| title_full_unstemmed | A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. |
| title_short | A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria. |
| title_sort | tcrβ repertoire signature can predict experimental cerebral malaria |
| url | http://europepmc.org/articles/PMC4742225?pdf=render |
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