Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil
Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler monkey (Alouatta caraya) kept in a colony at the Primatology Center of Rio de Janeiro State, Brazil, and it was genotypically characterized based on using PCR-RFLP and Microsatellite Analysis (MS), later...
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| Format: | Article |
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Elsevier
2022-12-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221322442200089X |
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| author | MRR Amendoeira IF Arruda SB Moreira DG Ubiali AS Barbosa HFJ Pena AHB Pereira CNS Raso TF Bonifácio YS Clemes TA Pissinatti AFA Santos A Pissinatti |
| author_facet | MRR Amendoeira IF Arruda SB Moreira DG Ubiali AS Barbosa HFJ Pena AHB Pereira CNS Raso TF Bonifácio YS Clemes TA Pissinatti AFA Santos A Pissinatti |
| author_sort | MRR Amendoeira |
| collection | DOAJ |
| description | Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler monkey (Alouatta caraya) kept in a colony at the Primatology Center of Rio de Janeiro State, Brazil, and it was genotypically characterized based on using PCR-RFLP and Microsatellite Analysis (MS), later on. T. gondii was successfully isolated from inocula deriving from heart, liver and tissue pool (heart, liver, lungs, axillary lymph nodes and cerebellum) samples. The isolate was named TgBgHmBrRJ1. The high virulence of the aforementioned strain was observed in infected mice. Non-archetypal genotype (ToxoDB PCR-RFLP #206) was obtained through PCR-RFLP. This genotype had been previously described in 12 isolates from different hosts, also in Southeastern Brazil, a fact that indicates likely high circulation of this genotype in this region. The isolate was also classified as non-archetypal, based on MS genotyping, as well as presented genotypic identity close to that of strains isolated from free-range non-symptomatic chickens (TgCkBr244,245,278,279) in Espírito Santo State. It is worth emphasizing that despite the large number of reports about clinical toxoplasmosis in neotropical primates in Brazil, this is just the second isolate of this parasite ever reported in this group of animals. |
| first_indexed | 2024-04-11T06:21:49Z |
| format | Article |
| id | doaj.art-6860de32443d4cd5b25e399ee914f72f |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-04-11T06:21:49Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-6860de32443d4cd5b25e399ee914f72f2022-12-22T04:40:32ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442022-12-0119187190Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in BrazilMRR Amendoeira0IF Arruda1SB Moreira2DG Ubiali3AS Barbosa4HFJ Pena5AHB Pereira6CNS Raso7TF Bonifácio8YS Clemes9TA Pissinatti10AFA Santos11A Pissinatti12Toxoplasmosis and Other Protozoan Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, Brazil; Corresponding author.Toxoplasmosis and Other Protozoan Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, BrazilRio de Janeiro Primatology Center, Environment State Institute, Estr. do Paraíso, s/n - Paraíso, Guapimirim, 25940-000, BrazilPathological Anatomy Sector, Epidemiology and Public Health Department, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR-465, Km 7, Seropédica, 23.897-000, BrazilToxoplasmosis and Other Protozoan Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, Brazil; Microbiology and Parasitology Department, Biomedical Institute, Federal Fluminense University, Rua Professor Hernani Melo, 101 / 212a-São Domingos, Niterói, 24210-130, BrazilPreventive Veterinary Medicine and Animal Health Department, Veterinary Medicine School, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, 05508-270, BrazilPathological Anatomy Sector, Epidemiology and Public Health Department, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR-465, Km 7, Seropédica, 23.897-000, BrazilToxoplasmosis and Other Protozoan Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, BrazilToxoplasmosis and Other Protozoan Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, BrazilPreventive Veterinary Medicine and Animal Health Department, Veterinary Medicine School, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, 05508-270, BrazilPrimatology Sector, Institute of Science and Technology in Biomodels, Fiocruz, Av. Brasil, 4365-Manguinhos, Rio de Janeiro, 21040-900, BrazilLaboratory of Viral Diversity and Diseases, Genetics Department, Biology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Ilha do Fundão, Centro de Ciências da Saúde, Bloco A, 21944-970, BrazilRio de Janeiro Primatology Center, Environment State Institute, Estr. do Paraíso, s/n - Paraíso, Guapimirim, 25940-000, BrazilToxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler monkey (Alouatta caraya) kept in a colony at the Primatology Center of Rio de Janeiro State, Brazil, and it was genotypically characterized based on using PCR-RFLP and Microsatellite Analysis (MS), later on. T. gondii was successfully isolated from inocula deriving from heart, liver and tissue pool (heart, liver, lungs, axillary lymph nodes and cerebellum) samples. The isolate was named TgBgHmBrRJ1. The high virulence of the aforementioned strain was observed in infected mice. Non-archetypal genotype (ToxoDB PCR-RFLP #206) was obtained through PCR-RFLP. This genotype had been previously described in 12 isolates from different hosts, also in Southeastern Brazil, a fact that indicates likely high circulation of this genotype in this region. The isolate was also classified as non-archetypal, based on MS genotyping, as well as presented genotypic identity close to that of strains isolated from free-range non-symptomatic chickens (TgCkBr244,245,278,279) in Espírito Santo State. It is worth emphasizing that despite the large number of reports about clinical toxoplasmosis in neotropical primates in Brazil, this is just the second isolate of this parasite ever reported in this group of animals.http://www.sciencedirect.com/science/article/pii/S221322442200089XNeotropical primates. toxoplasmosis. bioassays. genotyping |
| spellingShingle | MRR Amendoeira IF Arruda SB Moreira DG Ubiali AS Barbosa HFJ Pena AHB Pereira CNS Raso TF Bonifácio YS Clemes TA Pissinatti AFA Santos A Pissinatti Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil International Journal for Parasitology: Parasites and Wildlife Neotropical primates. toxoplasmosis. bioassays. genotyping |
| title | Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil |
| title_full | Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil |
| title_fullStr | Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil |
| title_full_unstemmed | Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil |
| title_short | Isolation and genetic characterization of Toxoplasma gondii from a captive black-and-gold howler monkey (Alouatta caraya Humboldt, 1812) in Brazil |
| title_sort | isolation and genetic characterization of toxoplasma gondii from a captive black and gold howler monkey alouatta caraya humboldt 1812 in brazil |
| topic | Neotropical primates. toxoplasmosis. bioassays. genotyping |
| url | http://www.sciencedirect.com/science/article/pii/S221322442200089X |
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