Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages
Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and tra...
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MDPI AG
2020-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/12/1422 |
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| author | Keiko Taniguchi-Ponciano Eduardo Peña-Martínez Gloria Silva-Román Sandra Vela-Patiño Ana Laura Guzman-Ortiz Hector Quezada Erick Gomez-Apo Laura Chavez-Macias Sophia Mercado-Medrez Guadalupe Vargas-Ortega Ana Laura Espinosa-de-los-Monteros Baldomero Gonzales-Virla Aldo Ferreira-Hermosillo Etual Espinosa-Cardenas Claudia Ramirez-Renteria Ernesto Sosa Blas Lopez-Felix Gerardo Guinto Daniel Marrero-Rodríguez Moises Mercado |
| author_facet | Keiko Taniguchi-Ponciano Eduardo Peña-Martínez Gloria Silva-Román Sandra Vela-Patiño Ana Laura Guzman-Ortiz Hector Quezada Erick Gomez-Apo Laura Chavez-Macias Sophia Mercado-Medrez Guadalupe Vargas-Ortega Ana Laura Espinosa-de-los-Monteros Baldomero Gonzales-Virla Aldo Ferreira-Hermosillo Etual Espinosa-Cardenas Claudia Ramirez-Renteria Ernesto Sosa Blas Lopez-Felix Gerardo Guinto Daniel Marrero-Rodríguez Moises Mercado |
| author_sort | Keiko Taniguchi-Ponciano |
| collection | DOAJ |
| description | Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA. Methods: Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events. Results: Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as <i>SRSF1</i>, <i>U2AF1</i> and <i>RBM42</i> among others were found in PA. These results were validated at mRNA level, which showed up-regulation of spliceosome genes in PA. Spliceosome-related genes segregate and categorize PA tumor subtypes. The PA showed alterations in <i>CDK18</i> and <i>THY1</i> mRNA isoforms which could be tumor specific. Conclusions: Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes. |
| first_indexed | 2024-03-10T14:30:23Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T14:30:23Z |
| publishDate | 2020-11-01 |
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| series | Genes |
| spelling | doaj.art-68662dd6a82c4886b815f382a53cadaa2023-11-20T22:38:27ZengMDPI AGGenes2073-44252020-11-011112142210.3390/genes11121422Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell LineagesKeiko Taniguchi-Ponciano0Eduardo Peña-Martínez1Gloria Silva-Román2Sandra Vela-Patiño3Ana Laura Guzman-Ortiz4Hector Quezada5Erick Gomez-Apo6Laura Chavez-Macias7Sophia Mercado-Medrez8Guadalupe Vargas-Ortega9Ana Laura Espinosa-de-los-Monteros10Baldomero Gonzales-Virla11Aldo Ferreira-Hermosillo12Etual Espinosa-Cardenas13Claudia Ramirez-Renteria14Ernesto Sosa15Blas Lopez-Felix16Gerardo Guinto17Daniel Marrero-Rodríguez18Moises Mercado19Unidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoLaboratorio de Investigacion en Inmunologia y Proteomica, Hospital Infantil de Mexico “Federico Gomez”, Mexico City 06720, MexicoLaboratorio de Investigacion en Inmunologia y Proteomica, Hospital Infantil de Mexico “Federico Gomez”, Mexico City 06720, MexicoÁrea de Neuropatología, Servicio de Anatomía Patológica, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México 06720, MexicoÁrea de Neuropatología, Servicio de Anatomía Patológica, Hospital General de México “Dr. Eduardo Liceaga”, Ciudad de México 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoServicio de Endocrinologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoServicio de Endocrinologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoServicio de Endocrinologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoServicio de Endocrinologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoServicio de Endocrinologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoServicio de Neurocirugia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoServicio de Neurocirugia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06720, MexicoCatedra CONACyT-Laboratorio de Endocrinologia Experimental, Unidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoUnidad de Investigación Medica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores, Mexico D.F. 06720, MexicoBackground: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA. Methods: Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events. Results: Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as <i>SRSF1</i>, <i>U2AF1</i> and <i>RBM42</i> among others were found in PA. These results were validated at mRNA level, which showed up-regulation of spliceosome genes in PA. Spliceosome-related genes segregate and categorize PA tumor subtypes. The PA showed alterations in <i>CDK18</i> and <i>THY1</i> mRNA isoforms which could be tumor specific. Conclusions: Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes.https://www.mdpi.com/2073-4425/11/12/1422pituitary adenomasalternative splicingmolecular markersmRNA isoforms |
| spellingShingle | Keiko Taniguchi-Ponciano Eduardo Peña-Martínez Gloria Silva-Román Sandra Vela-Patiño Ana Laura Guzman-Ortiz Hector Quezada Erick Gomez-Apo Laura Chavez-Macias Sophia Mercado-Medrez Guadalupe Vargas-Ortega Ana Laura Espinosa-de-los-Monteros Baldomero Gonzales-Virla Aldo Ferreira-Hermosillo Etual Espinosa-Cardenas Claudia Ramirez-Renteria Ernesto Sosa Blas Lopez-Felix Gerardo Guinto Daniel Marrero-Rodríguez Moises Mercado Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages Genes pituitary adenomas alternative splicing molecular markers mRNA isoforms |
| title | Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages |
| title_full | Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages |
| title_fullStr | Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages |
| title_full_unstemmed | Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages |
| title_short | Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from <i>POU1F1</i>- and <i>NR5A1</i>-Cell Lineages |
| title_sort | proteomic and transcriptomic analysis identify spliceosome as a significant component of the molecular machinery in the pituitary tumors derived from i pou1f1 i and i nr5a1 i cell lineages |
| topic | pituitary adenomas alternative splicing molecular markers mRNA isoforms |
| url | https://www.mdpi.com/2073-4425/11/12/1422 |
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