Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity

Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its activity against B. subtilis ATCC 9466 dan S. typhi ATCC 1408 has been done. The dried ground leaves (300.04 gram) were macerated with petroleum ether, chlorofor...

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Main Authors: Maulita Cut Nuria, Wahyono, Ratna Asmah Susidarti
Format: Article
Language:English
Published: Universitas Gadjah Mada 2011-01-01
Series:Indonesian Journal of Pharmacy
Subjects:
Online Access:http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/481/360
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author Maulita Cut Nuria
Wahyono
Ratna Asmah Susidarti
author_facet Maulita Cut Nuria
Wahyono
Ratna Asmah Susidarti
author_sort Maulita Cut Nuria
collection DOAJ
description Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its activity against B. subtilis ATCC 9466 dan S. typhi ATCC 1408 has been done. The dried ground leaves (300.04 gram) were macerated with petroleum ether, chloroform and methanol respectively. The methanol extract was the most active extract against both bacteria. Further separations of this extract using preparative thin layer chromatography yielded flavonoid glycoside. Its aglicon was identified as kaempferol based on its spectroscopic data (MS, IR, and UV-Vis) and UV spectrum (densitometry). This compound shows iradical zone of 6.62 mm (Bacillus subtilis) and 6.27 mm (Salmonella typhi) at concentration of 200 µg/disk.
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spelling doaj.art-6866744bfd86458d9b78390faa3e48412022-12-21T17:17:08ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862011-01-0122118Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activityMaulita Cut Nuria0Wahyono1Ratna Asmah Susidarti2Fakultas Farmasi, Universitas Wahid Hasyim SemarangFakultas Farmasi, Universitas Gadjah Mada JogjakartaFakultas Farmasi, Universitas Gadjah Mada JogjakartaIsolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its activity against B. subtilis ATCC 9466 dan S. typhi ATCC 1408 has been done. The dried ground leaves (300.04 gram) were macerated with petroleum ether, chloroform and methanol respectively. The methanol extract was the most active extract against both bacteria. Further separations of this extract using preparative thin layer chromatography yielded flavonoid glycoside. Its aglicon was identified as kaempferol based on its spectroscopic data (MS, IR, and UV-Vis) and UV spectrum (densitometry). This compound shows iradical zone of 6.62 mm (Bacillus subtilis) and 6.27 mm (Salmonella typhi) at concentration of 200 µg/disk.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/481/360kaempferoljangkangglycosideaglicon
spellingShingle Maulita Cut Nuria
Wahyono
Ratna Asmah Susidarti
Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
Indonesian Journal of Pharmacy
kaempferol
jangkang
glycoside
aglicon
title Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
title_full Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
title_fullStr Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
title_full_unstemmed Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
title_short Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity
title_sort isolation and identification of kaempferol from jangkang homalocladium platycladum f muell bailey leaves and its antibacterial activity
topic kaempferol
jangkang
glycoside
aglicon
url http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/481/360
work_keys_str_mv AT maulitacutnuria isolationandidentificationofkaempferolfromjangkanghomalocladiumplatycladumfmuellbaileyleavesanditsantibacterialactivity
AT wahyono isolationandidentificationofkaempferolfromjangkanghomalocladiumplatycladumfmuellbaileyleavesanditsantibacterialactivity
AT ratnaasmahsusidarti isolationandidentificationofkaempferolfromjangkanghomalocladiumplatycladumfmuellbaileyleavesanditsantibacterialactivity