Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles
Biochemical biomarkers and bioassays, due to their assumed immediate response after acute exposure of the organism to the stressor, are useful tools to gauging anthropogenic impacts. The toxicity of TiO2-nanoparticles (TiO2-NPs) on the Glyphodes pyloalis Walker was assessed and the LC50 value obtain...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2014-02-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | http://www.isj.unimo.it/articoli/ISJ321.pdf |
| _version_ | 1818508036590796800 |
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| author | N Memarizadeh M Ghadamyari M Adeli K Talebi |
| author_facet | N Memarizadeh M Ghadamyari M Adeli K Talebi |
| author_sort | N Memarizadeh |
| collection | DOAJ |
| description | Biochemical biomarkers and bioassays, due to their assumed immediate response after acute exposure of the organism to the stressor, are useful tools to gauging anthropogenic impacts. The toxicity of TiO2-nanoparticles (TiO2-NPs) on the Glyphodes pyloalis Walker was assessed and the LC50 value obtained as 660.85 mg/L. The in vivo responses of G. pyloalis to sub-lethal concentrations of TiO2-NPs were surveyed by monitoring the activity of general esterases (EST), peroxidase (POD) and glutathione S-transferase (GST), as biochemical biomarkers. Activity of these biomarkers affected by exposure to TiO2-NPs and this could lead to the mortality or sub-lethal impacts. The effect of TiO2-NPs concentrations on the activity of these enzymes was correlated to the exposure time. The activity of EST and GST was significantly decreased compared to the control, after 24 h of treatments. By increasing exposure time, the expression of EST and GST was significantly increased. More POD expression was occurred at low concentrations (i.e. LC20 and LC30); however, at high concentrations, less POD activity obtained. It can be concluded that these enzymes are good early indicator of toxicity and in conjunction with acute toxicity studies allow adverse effects of TiO2-NPs to be predicted and managed. |
| first_indexed | 2024-12-10T22:26:20Z |
| format | Article |
| id | doaj.art-686721b589c94c009b6851cce11499f1 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-10T22:26:20Z |
| publishDate | 2014-02-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-686721b589c94c009b6851cce11499f12022-12-22T01:31:11ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2014-02-01114753Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticlesN Memarizadeh0M Ghadamyari1M Adeli2K Talebi3Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Chemistry, Faculty of Science, University of Lorestan, Khoramabad, Iran; Department of Chemistry, Sharif University of Technology, Tehran, IranDepartment of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, IranBiochemical biomarkers and bioassays, due to their assumed immediate response after acute exposure of the organism to the stressor, are useful tools to gauging anthropogenic impacts. The toxicity of TiO2-nanoparticles (TiO2-NPs) on the Glyphodes pyloalis Walker was assessed and the LC50 value obtained as 660.85 mg/L. The in vivo responses of G. pyloalis to sub-lethal concentrations of TiO2-NPs were surveyed by monitoring the activity of general esterases (EST), peroxidase (POD) and glutathione S-transferase (GST), as biochemical biomarkers. Activity of these biomarkers affected by exposure to TiO2-NPs and this could lead to the mortality or sub-lethal impacts. The effect of TiO2-NPs concentrations on the activity of these enzymes was correlated to the exposure time. The activity of EST and GST was significantly decreased compared to the control, after 24 h of treatments. By increasing exposure time, the expression of EST and GST was significantly increased. More POD expression was occurred at low concentrations (i.e. LC20 and LC30); however, at high concentrations, less POD activity obtained. It can be concluded that these enzymes are good early indicator of toxicity and in conjunction with acute toxicity studies allow adverse effects of TiO2-NPs to be predicted and managed.http://www.isj.unimo.it/articoli/ISJ321.pdfTiO2-NPs; biomarker; Glyphodes pyloalis; esterases; glutathione S-transferase; peroxidase |
| spellingShingle | N Memarizadeh M Ghadamyari M Adeli K Talebi Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles Invertebrate Survival Journal TiO2-NPs; biomarker; Glyphodes pyloalis; esterases; glutathione S-transferase; peroxidase |
| title | Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles |
| title_full | Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles |
| title_fullStr | Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles |
| title_full_unstemmed | Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles |
| title_short | Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles |
| title_sort | biochemical biomarkers of glyphodes pyloalis walker lepidoptera pyralidae in exposure to tio2 nanoparticles |
| topic | TiO2-NPs; biomarker; Glyphodes pyloalis; esterases; glutathione S-transferase; peroxidase |
| url | http://www.isj.unimo.it/articoli/ISJ321.pdf |
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