Characteristics of gastric microbiota in cirrhotic patients with portal hypertension
Objective To investigate the structure characteristics, influencing factors and the correlation with clinical parameters of gastric microbiota in cirrhotic patients with portal hypertension. Methods Preoperative gastric mucosa and gastric juice samples were collected from cirrhotic patients undergoi...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2022-08-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202205060.htm |
| _version_ | 1828428506809237504 |
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| author | MU Zhiyong ZHOU Yinbin XIAO Xiao WANG Jun WEN Liangzhi CHEN Dongfeng |
| author_facet | MU Zhiyong ZHOU Yinbin XIAO Xiao WANG Jun WEN Liangzhi CHEN Dongfeng |
| author_sort | MU Zhiyong |
| collection | DOAJ |
| description | Objective To investigate the structure characteristics, influencing factors and the correlation with clinical parameters of gastric microbiota in cirrhotic patients with portal hypertension. Methods Preoperative gastric mucosa and gastric juice samples were collected from cirrhotic patients undergoing transjugular intrahepatic portosystemic shunt (TIPS) in our hospital from November 2020 to November 2021, and relevant clinical parameters during hospitalization were also obtained. The composition and diversity of gastric microbiota in the patients were analyzed by 16S rRNA high-throughput sequencing. LEfSe analysis was used to search for different bacteria; Redundancy analysis (RDA) was performed to identify the main factors affecting the gastric microbiota community structure; and Spearman correlation analysis was adopted to explore the correlation between the abundance of gastric microflora and clinical parameters. Results Proteobacteria, Actinobacteria, Acidobacteria and Chloroflexi were enriched in gastric mucosa, while Firmicutes and Fusobacteria in gastric juice. The Chao1 index of species in gastric mucosa was significantly higher than that in gastric fluid (P < 0.01), however, the Shannon index of community between the 2 groups was not different (P>0.05). PCoA analysis showed that there was statistical difference in community composition between gastric mucosa and gastric juice (P < 0.05). Further analysis of the gastric microbiota indicated that Peptostreptococcus and Capnocytophaga were abundant in the gastric mucosa of patients with portal hypertensive gastropathy (PHG), but the α diversity and β diversity of gastric mucosal flora had no significance between PHG and non-PHG patients (P>0.05). RDA analysis suggested that portal pressure gradient (PPG) was the main factor affecting the community structure of gastric mucosa flora (r2=0.683, P < 0.001), and the abundance of Hamadaea and Eikenella were positively correlated with PPG (r=0.633, 0.618, respectively; P < 0.05). Conclusion The microflora structure between gastric mucosa and gastric juice is different in cirrhotic patients with portal hypertension, but there is no significant difference in the species diversity. The composition of gastric mucosal flora in patients with PHG has characteristic changes, and PPG plays an important role in the structure of gastric mucosa microbiota. The relatively increased abundance of Hamadaea and Eikenella in gastric mucosa may be associated with portal hypertension in cirrhosis.
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| first_indexed | 2024-12-10T17:19:31Z |
| format | Article |
| id | doaj.art-68697e92e848400fa0feddc54960dbc9 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-12-10T17:19:31Z |
| publishDate | 2022-08-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-68697e92e848400fa0feddc54960dbc92022-12-22T01:40:02ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272022-08-0144151576158410.16016/j.2097-0927.202205060Characteristics of gastric microbiota in cirrhotic patients with portal hypertensionMU Zhiyong0 ZHOU Yinbin1XIAO Xiao2WANG Jun3WEN Liangzhi4CHEN Dongfeng5Department of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaDepartment of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaDepartment of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaDepartment of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaDepartment of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaDepartment of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, ChinaObjective To investigate the structure characteristics, influencing factors and the correlation with clinical parameters of gastric microbiota in cirrhotic patients with portal hypertension. Methods Preoperative gastric mucosa and gastric juice samples were collected from cirrhotic patients undergoing transjugular intrahepatic portosystemic shunt (TIPS) in our hospital from November 2020 to November 2021, and relevant clinical parameters during hospitalization were also obtained. The composition and diversity of gastric microbiota in the patients were analyzed by 16S rRNA high-throughput sequencing. LEfSe analysis was used to search for different bacteria; Redundancy analysis (RDA) was performed to identify the main factors affecting the gastric microbiota community structure; and Spearman correlation analysis was adopted to explore the correlation between the abundance of gastric microflora and clinical parameters. Results Proteobacteria, Actinobacteria, Acidobacteria and Chloroflexi were enriched in gastric mucosa, while Firmicutes and Fusobacteria in gastric juice. The Chao1 index of species in gastric mucosa was significantly higher than that in gastric fluid (P < 0.01), however, the Shannon index of community between the 2 groups was not different (P>0.05). PCoA analysis showed that there was statistical difference in community composition between gastric mucosa and gastric juice (P < 0.05). Further analysis of the gastric microbiota indicated that Peptostreptococcus and Capnocytophaga were abundant in the gastric mucosa of patients with portal hypertensive gastropathy (PHG), but the α diversity and β diversity of gastric mucosal flora had no significance between PHG and non-PHG patients (P>0.05). RDA analysis suggested that portal pressure gradient (PPG) was the main factor affecting the community structure of gastric mucosa flora (r2=0.683, P < 0.001), and the abundance of Hamadaea and Eikenella were positively correlated with PPG (r=0.633, 0.618, respectively; P < 0.05). Conclusion The microflora structure between gastric mucosa and gastric juice is different in cirrhotic patients with portal hypertension, but there is no significant difference in the species diversity. The composition of gastric mucosal flora in patients with PHG has characteristic changes, and PPG plays an important role in the structure of gastric mucosa microbiota. The relatively increased abundance of Hamadaea and Eikenella in gastric mucosa may be associated with portal hypertension in cirrhosis. http://aammt.tmmu.edu.cn/Upload/rhtml/202205060.htmliver cirrhosisportal hypertensionportal hypertensive gastropathygastric microbiota16s rrna |
| spellingShingle | MU Zhiyong ZHOU Yinbin XIAO Xiao WANG Jun WEN Liangzhi CHEN Dongfeng Characteristics of gastric microbiota in cirrhotic patients with portal hypertension 陆军军医大学学报 liver cirrhosis portal hypertension portal hypertensive gastropathy gastric microbiota 16s rrna |
| title | Characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| title_full | Characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| title_fullStr | Characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| title_full_unstemmed | Characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| title_short | Characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| title_sort | characteristics of gastric microbiota in cirrhotic patients with portal hypertension |
| topic | liver cirrhosis portal hypertension portal hypertensive gastropathy gastric microbiota 16s rrna |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202205060.htm |
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