Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China
Objectives: To reveal the clinical and molecular characteristics of Bordetella pertussis (BP) prevalent in Shanghai, China. Methods: A total of 9430 children with suspected pertussis from 2021 to 2022 were included, and nasopharyngeal swab samples were collected for polymerase chain reaction detecti...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223000048 |
| _version_ | 1797897300651016192 |
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| author | Pan Fu Jinlan Zhou Jianhua Meng Zhiwei Liu Yaxier Nijiati Leiyan He Chunling Li Saige Chen Aimin Wang Gangfeng Yan Guoping Lu Lijun Zhou Xiaowen Zhai Chuanqing Wang |
| author_facet | Pan Fu Jinlan Zhou Jianhua Meng Zhiwei Liu Yaxier Nijiati Leiyan He Chunling Li Saige Chen Aimin Wang Gangfeng Yan Guoping Lu Lijun Zhou Xiaowen Zhai Chuanqing Wang |
| author_sort | Pan Fu |
| collection | DOAJ |
| description | Objectives: To reveal the clinical and molecular characteristics of Bordetella pertussis (BP) prevalent in Shanghai, China. Methods: A total of 9430 children with suspected pertussis from 2021 to 2022 were included, and nasopharyngeal swab samples were collected for polymerase chain reaction detection, culture, antimicrobial susceptibility testing, and 23S rRNA gene A2047G detection. BP strains were typed using multilocus variable-number tandem-repeat analysis and virulence genotyping. Results: Of 9430 cases, 5.1% and 1.6% were confirmed by polymerase chain reaction and culture, respectively. Infants (aged <1 year) accounted for 24.7% and presented much more severe symptoms than noninfants. Pertussis was most frequently detected in infants aged 0-6 months (11.3∼14.0%) and children aged >6-10 years (10.8∼21.7%). Macrolide-resistant BP (MRBP) accounted for 89.3%, and all carried the A2047G mutation. There were six multilocus variable-number tandem-repeat analysis types (MTs), including MT28 (62.0%), MT195 (20%), MT27 (10.0%), MT104 (4.7%), MT55 (2.7%), and MT32 (0.7%). BP strains with pertussis toxin (ptx)P3/(pertactin) prn2/ptxC2/ptxA1/(fimbrial proteins) fim2-1/fim3-1, including MT27, MT28, and MT32, accounted for 72.7%, among which MT27 and MT32 were macrolide-sensitive BP, whereas most (94.6∼100%) of MT28 were MRBP. Strains harboring ptxP1/prn1/ptxC1/ptxA1/fim2-1/fim3-1, including MT55, MT104, and MT195, belonged to macrolide-sensitive BP. Conclusion: The emergence and spread of MT28 ptxP3-MRBP was first reported in China, highlighting the importance of continuous surveillance of ptxP3-MRBP to prevent its potential circulation. |
| first_indexed | 2024-04-10T07:56:24Z |
| format | Article |
| id | doaj.art-68707c5c9f5a4054a45f55696d99674d |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-04-10T07:56:24Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-68707c5c9f5a4054a45f55696d99674d2023-02-23T04:30:28ZengElsevierInternational Journal of Infectious Diseases1201-97122023-03-01128205211Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in ChinaPan Fu0Jinlan Zhou1Jianhua Meng2Zhiwei Liu3Yaxier Nijiati4Leiyan He5Chunling Li6Saige Chen7Aimin Wang8Gangfeng Yan9Guoping Lu10Lijun Zhou11Xiaowen Zhai12Chuanqing Wang13Department of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China; Nosocomial Infection Control Department, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaPediatric intensive care unit, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Hematology and Oncology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Clinical Laboratory, Taian Maternity and Child Health Hospital, Taian, Shandong Province, ChinaOrthopedics Department, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaPediatric intensive care unit, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaPediatric intensive care unit, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaOutpatient and Emergency Management Office, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China; Corresponding authors: Wang Chuanqing, Tel: 0086-021-64931060, Xiaowen Zhai, Tel: 0086-021-64931060, Lijun Zhou, Tel: 0086-021-64931060Department of Hematology and Oncology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China; Corresponding authors: Wang Chuanqing, Tel: 0086-021-64931060, Xiaowen Zhai, Tel: 0086-021-64931060, Lijun Zhou, Tel: 0086-021-64931060Department of Clinical Microbiology Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China; Nosocomial Infection Control Department, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China; Corresponding authors: Wang Chuanqing, Tel: 0086-021-64931060, Xiaowen Zhai, Tel: 0086-021-64931060, Lijun Zhou, Tel: 0086-021-64931060Objectives: To reveal the clinical and molecular characteristics of Bordetella pertussis (BP) prevalent in Shanghai, China. Methods: A total of 9430 children with suspected pertussis from 2021 to 2022 were included, and nasopharyngeal swab samples were collected for polymerase chain reaction detection, culture, antimicrobial susceptibility testing, and 23S rRNA gene A2047G detection. BP strains were typed using multilocus variable-number tandem-repeat analysis and virulence genotyping. Results: Of 9430 cases, 5.1% and 1.6% were confirmed by polymerase chain reaction and culture, respectively. Infants (aged <1 year) accounted for 24.7% and presented much more severe symptoms than noninfants. Pertussis was most frequently detected in infants aged 0-6 months (11.3∼14.0%) and children aged >6-10 years (10.8∼21.7%). Macrolide-resistant BP (MRBP) accounted for 89.3%, and all carried the A2047G mutation. There were six multilocus variable-number tandem-repeat analysis types (MTs), including MT28 (62.0%), MT195 (20%), MT27 (10.0%), MT104 (4.7%), MT55 (2.7%), and MT32 (0.7%). BP strains with pertussis toxin (ptx)P3/(pertactin) prn2/ptxC2/ptxA1/(fimbrial proteins) fim2-1/fim3-1, including MT27, MT28, and MT32, accounted for 72.7%, among which MT27 and MT32 were macrolide-sensitive BP, whereas most (94.6∼100%) of MT28 were MRBP. Strains harboring ptxP1/prn1/ptxC1/ptxA1/fim2-1/fim3-1, including MT55, MT104, and MT195, belonged to macrolide-sensitive BP. Conclusion: The emergence and spread of MT28 ptxP3-MRBP was first reported in China, highlighting the importance of continuous surveillance of ptxP3-MRBP to prevent its potential circulation.http://www.sciencedirect.com/science/article/pii/S1201971223000048Bordetella pertussisMT28ptxP3MRBPChina |
| spellingShingle | Pan Fu Jinlan Zhou Jianhua Meng Zhiwei Liu Yaxier Nijiati Leiyan He Chunling Li Saige Chen Aimin Wang Gangfeng Yan Guoping Lu Lijun Zhou Xiaowen Zhai Chuanqing Wang Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China International Journal of Infectious Diseases Bordetella pertussis MT28 ptxP3 MRBP China |
| title | Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China |
| title_full | Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China |
| title_fullStr | Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China |
| title_full_unstemmed | Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China |
| title_short | Emergence and spread of MT28 ptxP3 allele macrolide-resistant Bordetella pertussis from 2021 to 2022 in China |
| title_sort | emergence and spread of mt28 ptxp3 allele macrolide resistant bordetella pertussis from 2021 to 2022 in china |
| topic | Bordetella pertussis MT28 ptxP3 MRBP China |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223000048 |
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