The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract
The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) wa...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Diponegoro University, Faculty of Animal and Agricultural Sciences
2019-12-01
|
| Series: | Journal of the Indonesian Tropical Animal Agriculture |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/25350 |
| _version_ | 1818309602935046144 |
|---|---|
| author | W. W. Mubarokah W. Nurcahyo J. Prastowo K. Kurniasih |
| author_facet | W. W. Mubarokah W. Nurcahyo J. Prastowo K. Kurniasih |
| author_sort | W. W. Mubarokah |
| collection | DOAJ |
| description | The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio), while the results of the SEM showed that there was cuticle damage and anterior labia abrasion at the dose of 53 mg/mL. The Areca catechu crude aqueous extract showed anthelmintic activity potential by reducing the number of the adult worms, lowering their protein profile and damaging the A. galli worms in the intestine. |
| first_indexed | 2024-12-13T07:32:47Z |
| format | Article |
| id | doaj.art-6876c7e076354d36a1c4eb7e8044c372 |
| institution | Directory Open Access Journal |
| issn | 2087-8273 2460-6278 |
| language | English |
| last_indexed | 2024-12-13T07:32:47Z |
| publishDate | 2019-12-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj.art-6876c7e076354d36a1c4eb7e8044c3722022-12-21T23:55:10ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782019-12-0144439239910.14710/jitaa.44.4.392-39916020The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extractW. W. Mubarokah0W. Nurcahyo1J. Prastowo2K. Kurniasih3Department of Animal Health, Agriculture Extension College, The Polytechnique of Agricultural Development of Yogyakarta Magelang Jalan Magelang-Kopeng Km 7 Purwosari, Tegalrejo, Magelang, Central Java 56192Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada Bulaksumur Sleman, Yogyakarta, 55281Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada Bulaksumur Sleman, Yogyakarta, 55281Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada Bulaksumur Sleman, Yogyakarta, 55281The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio), while the results of the SEM showed that there was cuticle damage and anterior labia abrasion at the dose of 53 mg/mL. The Areca catechu crude aqueous extract showed anthelmintic activity potential by reducing the number of the adult worms, lowering their protein profile and damaging the A. galli worms in the intestine.https://ejournal.undip.ac.id/index.php/jitaa/article/view/25350ascaridia galliareca catechusemsds-page |
| spellingShingle | W. W. Mubarokah W. Nurcahyo J. Prastowo K. Kurniasih The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract Journal of the Indonesian Tropical Animal Agriculture ascaridia galli areca catechu sem sds-page |
| title | The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract |
| title_full | The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract |
| title_fullStr | The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract |
| title_full_unstemmed | The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract |
| title_short | The population, protein profile and ultrastructure of Ascaridia galli in chicken treated using Areca catechu crude aqueous extract |
| title_sort | population protein profile and ultrastructure of ascaridia galli in chicken treated using areca catechu crude aqueous extract |
| topic | ascaridia galli areca catechu sem sds-page |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/25350 |
| work_keys_str_mv | AT wwmubarokah thepopulationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT wnurcahyo thepopulationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT jprastowo thepopulationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT kkurniasih thepopulationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT wwmubarokah populationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT wnurcahyo populationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT jprastowo populationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract AT kkurniasih populationproteinprofileandultrastructureofascaridiagalliinchickentreatedusingarecacatechucrudeaqueousextract |