Minimally invasive blood sampling method for genetic studies on Gopherus tortoises
Obtaining good quality tissue samples is the first hurdle in any molecular study. This is especially true for studies involving management and conservation of wild fauna. In the case of tortoises, the most common sources of DNA are blood samples. However, only a minimal amount of blood is required f...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Museu de Ciències Naturals de Barcelona
2015-04-01
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| Series: | Animal Biodiversity and Conservation |
| Subjects: | |
| Online Access: | http://abc.museucienciesjournals.cat/files/ABC_38-1_pp_31-35.pdf |
| _version_ | 1818030788255416320 |
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| author | García–Feria, L. M. Ureña–Aranda, C. A. Espinosa de los Monteros, A., |
| author_facet | García–Feria, L. M. Ureña–Aranda, C. A. Espinosa de los Monteros, A., |
| author_sort | García–Feria, L. M. |
| collection | DOAJ |
| description | Obtaining good quality tissue samples is the first hurdle in any molecular study. This is especially true for studies involving management and conservation of wild fauna. In the case of tortoises, the most common sources of DNA are blood samples. However, only a minimal amount of blood is required for PCR assays. Samples are obtained mainly from the brachial and jugular vein after restraining the animal chemically, or from conscious individuals by severe handling methods and clamping. Herein, we present a minimally invasive technique that has proven effective for extracting small quantities of blood, suitable for genetic analyses. Furthermore, the samples obtained yielded better DNA amplification than other cell sources, such as cloacal epithelium cells. After two years of use on wild tortoises,
this technique has shown to be harmless. We suggest that sampling a small amount of blood could also be useful for other types of analyses, such as physiologic and medical monitoring. |
| first_indexed | 2024-12-10T05:41:09Z |
| format | Article |
| id | doaj.art-687a091389e842ac8bd705ef28e33590 |
| institution | Directory Open Access Journal |
| issn | 1578-665X |
| language | English |
| last_indexed | 2024-12-10T05:41:09Z |
| publishDate | 2015-04-01 |
| publisher | Museu de Ciències Naturals de Barcelona |
| record_format | Article |
| series | Animal Biodiversity and Conservation |
| spelling | doaj.art-687a091389e842ac8bd705ef28e335902022-12-22T02:00:17ZengMuseu de Ciències Naturals de BarcelonaAnimal Biodiversity and Conservation1578-665X2015-04-013813135Minimally invasive blood sampling method for genetic studies on Gopherus tortoisesGarcía–Feria, L. M.0Ureña–Aranda, C. A.1Espinosa de los Monteros, A.,2 Inst. de Ecología A. C., México Inst. de Ecología A. C., México Inst. de Ecología A. C., MéxicoObtaining good quality tissue samples is the first hurdle in any molecular study. This is especially true for studies involving management and conservation of wild fauna. In the case of tortoises, the most common sources of DNA are blood samples. However, only a minimal amount of blood is required for PCR assays. Samples are obtained mainly from the brachial and jugular vein after restraining the animal chemically, or from conscious individuals by severe handling methods and clamping. Herein, we present a minimally invasive technique that has proven effective for extracting small quantities of blood, suitable for genetic analyses. Furthermore, the samples obtained yielded better DNA amplification than other cell sources, such as cloacal epithelium cells. After two years of use on wild tortoises, this technique has shown to be harmless. We suggest that sampling a small amount of blood could also be useful for other types of analyses, such as physiologic and medical monitoring.http://abc.museucienciesjournals.cat/files/ABC_38-1_pp_31-35.pdfBlood extractionDNA sourceTortoises |
| spellingShingle | García–Feria, L. M. Ureña–Aranda, C. A. Espinosa de los Monteros, A., Minimally invasive blood sampling method for genetic studies on Gopherus tortoises Animal Biodiversity and Conservation Blood extraction DNA source Tortoises |
| title | Minimally invasive blood sampling method for genetic studies on Gopherus tortoises |
| title_full | Minimally invasive blood sampling method for genetic studies on Gopherus tortoises |
| title_fullStr | Minimally invasive blood sampling method for genetic studies on Gopherus tortoises |
| title_full_unstemmed | Minimally invasive blood sampling method for genetic studies on Gopherus tortoises |
| title_short | Minimally invasive blood sampling method for genetic studies on Gopherus tortoises |
| title_sort | minimally invasive blood sampling method for genetic studies on gopherus tortoises |
| topic | Blood extraction DNA source Tortoises |
| url | http://abc.museucienciesjournals.cat/files/ABC_38-1_pp_31-35.pdf |
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