Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei)
To characterize the cold tolerance mechanism of the Pacific white shrimp (Litopenaeus vannamei), we performed single-cell RNA sequencing (scRNA-seq) of ∼5185 hepatopancreas cells from cold-tolerant (Lv-T) and common (Lv-C) L. vannamei at preferred and low temperatures (28°C and 10°C, respectively)....
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.792172/full |
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| author | Weilin Zhu Chunling Yang Xiuli Chen Qingyun Liu Qingyun Liu Qiangyong Li Qiangyong Li Min Peng Huanling Wang Xiaohan Chen Qiong Yang Zhenping Liao Min Li Chuanyan Pan Pengfei Feng Digang Zeng Yongzhen Zhao Yongzhen Zhao |
| author_facet | Weilin Zhu Chunling Yang Xiuli Chen Qingyun Liu Qingyun Liu Qiangyong Li Qiangyong Li Min Peng Huanling Wang Xiaohan Chen Qiong Yang Zhenping Liao Min Li Chuanyan Pan Pengfei Feng Digang Zeng Yongzhen Zhao Yongzhen Zhao |
| author_sort | Weilin Zhu |
| collection | DOAJ |
| description | To characterize the cold tolerance mechanism of the Pacific white shrimp (Litopenaeus vannamei), we performed single-cell RNA sequencing (scRNA-seq) of ∼5185 hepatopancreas cells from cold-tolerant (Lv-T) and common (Lv-C) L. vannamei at preferred and low temperatures (28°C and 10°C, respectively). The cells fell into 10 clusters and 4 cell types: embryonic, resorptive, blister-like, and fibrillar. We identified differentially expressed genes between Lv-T and Lv-C, which were mainly associated with the terms “immune system,” “cytoskeleton,” “antioxidant system,” “digestive enzyme,” and “detoxification,” as well as the pathways “metabolic pathways of oxidative phosphorylation,” “metabolism of xenobiotics by cytochrome P450,” “chemical carcinogenesis,” “drug metabolism-cytochrome P450,” and “fatty acid metabolism.” Reconstruction of fibrillar cell trajectories showed that, under low temperature stress, hepatopancreas cells had two distinct fates, cell fate 1 and cell fate 2. Cell fate 1 was mainly involved in signal transduction and sensory organ development. Cell fate 2 was mainly involved in metabolic processes. This study preliminarily clarifies the molecular mechanisms underlying cold tolerance in L. vannamei, which will be useful for the breeding of shrimp with greater cold tolerance. |
| first_indexed | 2024-12-20T13:22:26Z |
| format | Article |
| id | doaj.art-687c47e8eb07478e8adf44a189340c4e |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-20T13:22:26Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-687c47e8eb07478e8adf44a189340c4e2022-12-21T19:39:21ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-01-011210.3389/fgene.2021.792172792172Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei)Weilin Zhu0Chunling Yang1Xiuli Chen2Qingyun Liu3Qingyun Liu4Qiangyong Li5Qiangyong Li6Min Peng7Huanling Wang8Xiaohan Chen9Qiong Yang10Zhenping Liao11Min Li12Chuanyan Pan13Pengfei Feng14Digang Zeng15Yongzhen Zhao16Yongzhen Zhao17Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Shrimp and Crab Breeding Engineering Technology Research Center, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Shrimp and Crab Breeding Engineering Technology Research Center, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaKey Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agriculture University, Wuhan, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, ChinaGuangxi Shrimp and Crab Breeding Engineering Technology Research Center, Guangxi Academy of Fishery Sciences, Nanning, ChinaTo characterize the cold tolerance mechanism of the Pacific white shrimp (Litopenaeus vannamei), we performed single-cell RNA sequencing (scRNA-seq) of ∼5185 hepatopancreas cells from cold-tolerant (Lv-T) and common (Lv-C) L. vannamei at preferred and low temperatures (28°C and 10°C, respectively). The cells fell into 10 clusters and 4 cell types: embryonic, resorptive, blister-like, and fibrillar. We identified differentially expressed genes between Lv-T and Lv-C, which were mainly associated with the terms “immune system,” “cytoskeleton,” “antioxidant system,” “digestive enzyme,” and “detoxification,” as well as the pathways “metabolic pathways of oxidative phosphorylation,” “metabolism of xenobiotics by cytochrome P450,” “chemical carcinogenesis,” “drug metabolism-cytochrome P450,” and “fatty acid metabolism.” Reconstruction of fibrillar cell trajectories showed that, under low temperature stress, hepatopancreas cells had two distinct fates, cell fate 1 and cell fate 2. Cell fate 1 was mainly involved in signal transduction and sensory organ development. Cell fate 2 was mainly involved in metabolic processes. This study preliminarily clarifies the molecular mechanisms underlying cold tolerance in L. vannamei, which will be useful for the breeding of shrimp with greater cold tolerance.https://www.frontiersin.org/articles/10.3389/fgene.2021.792172/fullsingle-cell RNA sequencingLitopenaeus vannameicold tolerancemechanismspacific white shrimp |
| spellingShingle | Weilin Zhu Chunling Yang Xiuli Chen Qingyun Liu Qingyun Liu Qiangyong Li Qiangyong Li Min Peng Huanling Wang Xiaohan Chen Qiong Yang Zhenping Liao Min Li Chuanyan Pan Pengfei Feng Digang Zeng Yongzhen Zhao Yongzhen Zhao Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) Frontiers in Genetics single-cell RNA sequencing Litopenaeus vannamei cold tolerance mechanisms pacific white shrimp |
| title | Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) |
| title_full | Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) |
| title_fullStr | Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) |
| title_full_unstemmed | Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) |
| title_short | Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus Vannamei) |
| title_sort | single cell ribonucleic acid sequencing clarifies cold tolerance mechanisms in the pacific white shrimp litopenaeus vannamei |
| topic | single-cell RNA sequencing Litopenaeus vannamei cold tolerance mechanisms pacific white shrimp |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.792172/full |
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