Development and validation of a GC–MS method for soybean organ-specific metabolomics
Field mold (FM) can easily deteriorate the preharvest soybean in the field, and Fusarium moniliforme is demonstrated as the dominant pathogenic fungi. Metabolomics is a powerful tool to reveal the resistance mechanism in response to microbial infection. Therefore, in this research, the Design of Exp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1343943X.2018.1488539 |
| _version_ | 1819000673630420992 |
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| author | Bao-yu Hu Cai-qiong Yang Nasir Iqbal Jun-cai Deng Jing Zhang Wen-yu Yang Jiang Liu |
| author_facet | Bao-yu Hu Cai-qiong Yang Nasir Iqbal Jun-cai Deng Jing Zhang Wen-yu Yang Jiang Liu |
| author_sort | Bao-yu Hu |
| collection | DOAJ |
| description | Field mold (FM) can easily deteriorate the preharvest soybean in the field, and Fusarium moniliforme is demonstrated as the dominant pathogenic fungi. Metabolomics is a powerful tool to reveal the resistance mechanism in response to microbial infection. Therefore, in this research, the Design of Experiment (DOE) model was developed to optimize the extraction solvent combinations for metabolomic study of soybean seed and pod based on gas chromatography–mass spectrometry (GC–MS). Combined with the number of extracted peaks and the peak area of common substances, the extraction efficiency of different solvent was analyzed by multivariate statistical analysis. The result showed that isopropanol/water/methanol (1:1:1 and 1:1:4, v/v/v) mixture was highly efficient for metabolites extractions of soybean seed and pod, respectively. Additionally, the potential metabolites and pathways concerned in FM resistance were explored by the optimized extraction solvent system based on metabolomics analysis. Amino acid metabolism in soybean seed was disturbed by F. moniliforme and metabolic pathways related to energy conversion in soybean pod strongly responded to fungal infection. This study constructs a GC–MS-based metabonomic method for soybean metabolites; comparative analysis of organ-specific metabolomics for soybean fruit could be further applied in soybean metabolomics researches. |
| first_indexed | 2024-12-20T22:37:03Z |
| format | Article |
| id | doaj.art-687dce1d4bbe4971bba61a8d918e503e |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-20T22:37:03Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-687dce1d4bbe4971bba61a8d918e503e2022-12-21T19:24:34ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082018-07-0121321522410.1080/1343943X.2018.14885391488539Development and validation of a GC–MS method for soybean organ-specific metabolomicsBao-yu Hu0Cai-qiong Yang1Nasir Iqbal2Jun-cai Deng3Jing Zhang4Wen-yu Yang5Jiang Liu6Ministry of AgricultureMinistry of AgricultureMinistry of AgricultureMinistry of AgricultureMinistry of AgricultureMinistry of AgricultureMinistry of AgricultureField mold (FM) can easily deteriorate the preharvest soybean in the field, and Fusarium moniliforme is demonstrated as the dominant pathogenic fungi. Metabolomics is a powerful tool to reveal the resistance mechanism in response to microbial infection. Therefore, in this research, the Design of Experiment (DOE) model was developed to optimize the extraction solvent combinations for metabolomic study of soybean seed and pod based on gas chromatography–mass spectrometry (GC–MS). Combined with the number of extracted peaks and the peak area of common substances, the extraction efficiency of different solvent was analyzed by multivariate statistical analysis. The result showed that isopropanol/water/methanol (1:1:1 and 1:1:4, v/v/v) mixture was highly efficient for metabolites extractions of soybean seed and pod, respectively. Additionally, the potential metabolites and pathways concerned in FM resistance were explored by the optimized extraction solvent system based on metabolomics analysis. Amino acid metabolism in soybean seed was disturbed by F. moniliforme and metabolic pathways related to energy conversion in soybean pod strongly responded to fungal infection. This study constructs a GC–MS-based metabonomic method for soybean metabolites; comparative analysis of organ-specific metabolomics for soybean fruit could be further applied in soybean metabolomics researches.http://dx.doi.org/10.1080/1343943X.2018.1488539Soybeanfungal infectionGC–MSmetabolomicsorgan specificity |
| spellingShingle | Bao-yu Hu Cai-qiong Yang Nasir Iqbal Jun-cai Deng Jing Zhang Wen-yu Yang Jiang Liu Development and validation of a GC–MS method for soybean organ-specific metabolomics Plant Production Science Soybean fungal infection GC–MS metabolomics organ specificity |
| title | Development and validation of a GC–MS method for soybean organ-specific metabolomics |
| title_full | Development and validation of a GC–MS method for soybean organ-specific metabolomics |
| title_fullStr | Development and validation of a GC–MS method for soybean organ-specific metabolomics |
| title_full_unstemmed | Development and validation of a GC–MS method for soybean organ-specific metabolomics |
| title_short | Development and validation of a GC–MS method for soybean organ-specific metabolomics |
| title_sort | development and validation of a gc ms method for soybean organ specific metabolomics |
| topic | Soybean fungal infection GC–MS metabolomics organ specificity |
| url | http://dx.doi.org/10.1080/1343943X.2018.1488539 |
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