Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea
<p>The expression pattern of connexin 43 (Cx43) in the cochlea is not determined and is controversial. Since the presence of Cx43 is essential for hearing, we re-examined its distribution during postnatal development of rat cochlea. Cx43 protein was expressed in spiral ganglion neurons (SGNs)...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2015-02-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | http://ejh.it/index.php/ejh/article/view/2464 |
| _version_ | 1811243891387531264 |
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| author | W.J. Liu J. Yang |
| author_facet | W.J. Liu J. Yang |
| author_sort | W.J. Liu |
| collection | DOAJ |
| description | <p>The expression pattern of connexin 43 (Cx43) in the cochlea is not determined and is controversial. Since the presence of Cx43 is essential for hearing, we re-examined its distribution during postnatal development of rat cochlea. Cx43 protein was expressed in spiral ganglion neurons (SGNs) and their neurite terminals innervating the inner and outer hair cells (IHCs and OHCs) as early as birth (post-natal day 0, P0), and persisted until P14. Double immunofluorescence staining, using two antibodies against Cx43 and TUJ1, a marker for all SGNs and afferent terminals, showed that immunoreactivity for Cx43 and TUJ1 was perfectly colocalized in SGNs and afferent terminals associated with the IHCs and OHCs. However, beyond P14, Cx43 immunostaining could no longer be detected in the region of the synaptic terminals at the bases of IHCs and OHCs (P17, adult). In contrast, Cx43 maintained its expression in SGNs into adulthood. We further performed quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) to identify the presence of Cx43 mRNA in the modiolus (mainly containing SGNs). Cx43 mRNA was higher at P8, compared with P1, and subsequently decreased at P14. These results indicated that Cx43 correlated with cochlear synaptogenesis and establishment of auditory neurotransmission.</p> |
| first_indexed | 2024-04-12T14:16:34Z |
| format | Article |
| id | doaj.art-687dd5a31c09430c936492f99f35a072 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-12T14:16:34Z |
| publishDate | 2015-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-687dd5a31c09430c936492f99f35a0722022-12-22T03:29:43ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062015-02-0159110.4081/ejh.2015.24641437Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochleaW.J. Liu0J. Yang1Xinhua Hospital, Shanghai Jiaotong University, Shanghai Jiaotong University Ear InstituteXinhua Hospital, Shanghai Jiaotong University, Shanghai Jiaotong University Ear Institute<p>The expression pattern of connexin 43 (Cx43) in the cochlea is not determined and is controversial. Since the presence of Cx43 is essential for hearing, we re-examined its distribution during postnatal development of rat cochlea. Cx43 protein was expressed in spiral ganglion neurons (SGNs) and their neurite terminals innervating the inner and outer hair cells (IHCs and OHCs) as early as birth (post-natal day 0, P0), and persisted until P14. Double immunofluorescence staining, using two antibodies against Cx43 and TUJ1, a marker for all SGNs and afferent terminals, showed that immunoreactivity for Cx43 and TUJ1 was perfectly colocalized in SGNs and afferent terminals associated with the IHCs and OHCs. However, beyond P14, Cx43 immunostaining could no longer be detected in the region of the synaptic terminals at the bases of IHCs and OHCs (P17, adult). In contrast, Cx43 maintained its expression in SGNs into adulthood. We further performed quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) to identify the presence of Cx43 mRNA in the modiolus (mainly containing SGNs). Cx43 mRNA was higher at P8, compared with P1, and subsequently decreased at P14. These results indicated that Cx43 correlated with cochlear synaptogenesis and establishment of auditory neurotransmission.</p>http://ejh.it/index.php/ejh/article/view/2464Connexin43•gap junction•development•cochlea•rat |
| spellingShingle | W.J. Liu J. Yang Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea European Journal of Histochemistry Connexin43•gap junction•development•cochlea•rat |
| title | Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea |
| title_full | Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea |
| title_fullStr | Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea |
| title_full_unstemmed | Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea |
| title_short | Preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post-natal rat cochlea |
| title_sort | preferentially regulated expression of connexin 43 in the developing spiral ganglion neurons and afferent terminals in post natal rat cochlea |
| topic | Connexin43•gap junction•development•cochlea•rat |
| url | http://ejh.it/index.php/ejh/article/view/2464 |
| work_keys_str_mv | AT wjliu preferentiallyregulatedexpressionofconnexin43inthedevelopingspiralganglionneuronsandafferentterminalsinpostnatalratcochlea AT jyang preferentiallyregulatedexpressionofconnexin43inthedevelopingspiralganglionneuronsandafferentterminalsinpostnatalratcochlea |