The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock
Microbe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term sur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00671/full |
| _version_ | 1817979899985526784 |
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| author | Natalie Leys Bo Byloos Bo Byloos Ilse Coninx Olivier Van Hoey Charles Cockell Natasha Nicholson Vyacheslav Ilyin Rob Van Houdt Nico Boon |
| author_facet | Natalie Leys Bo Byloos Bo Byloos Ilse Coninx Olivier Van Hoey Charles Cockell Natasha Nicholson Vyacheslav Ilyin Rob Van Houdt Nico Boon |
| author_sort | Natalie Leys |
| collection | DOAJ |
| description | Microbe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term survival of Cupriavidus metallidurans CH34 in mineral water and the interaction with basalt, a lunar-type rock in preparation for the ESA spaceflight experiment, BIOROCK. Therefore, C. metallidurans CH34 cells were suspended in mineral water supplemented with or without crushed basalt and send for 3 months on board the Russian FOTON-M4 capsule. Long-term storage had a significant impact on cell physiology and energy status (by flow cytometry analysis, plate count and intracellular ATP measurements) as 60% of cells stored on ground lost their cell membrane potential, only 17% were still active, average ATP levels per cell were significantly lower and cultivability dropped to 1%. The cells stored in the presence of basalt and exposed to space flight conditions during storage however showed less dramatic changes in physiology, with only 16% of the cells lost their cell membrane potential and 24% were still active, leading to a higher cultivability (50%) and indicating a general positive effect of basalt and space flight on survival. Microbe-mineral interactions and biofilm formation was altered by spaceflight as less biofilm was formed on the basalt during flight conditions. Leaching from basalt also changed (measured with ICP-OES), showing that cells release more copper from basalt and the presence of cells also impacted iron and magnesium concentration irrespective of the presence of basalt. The flight conditions thus could counteract some of the detrimental effects observed after the 3 month storage conditions. |
| first_indexed | 2024-04-13T22:48:23Z |
| format | Article |
| id | doaj.art-6881a3ab60f14d27ba4454db774139d3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T22:48:23Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6881a3ab60f14d27ba4454db774139d32022-12-22T02:26:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00671259118The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog RockNatalie Leys0Bo Byloos1Bo Byloos2Ilse Coninx3Olivier Van Hoey4Charles Cockell5Natasha Nicholson6Vyacheslav Ilyin7Rob Van Houdt8Nico Boon9Microbiology Unit, Belgian Nuclear Research Centre, SCK•CENMol, BelgiumMicrobiology Unit, Belgian Nuclear Research Centre, SCK•CENMol, BelgiumCenter for Microbial Ecology and Technology, Ghent UniversityGhent, BelgiumMicrobiology Unit, Belgian Nuclear Research Centre, SCK•CENMol, BelgiumResearch in Dosimetric Applications, Belgian Nuclear Research Centre, SCK•CENMol, BelgiumUK Centre for Astrobiology, School of Physics and Astronomy, University of EdinburghEdinburgh, UKUK Centre for Astrobiology, School of Physics and Astronomy, University of EdinburghEdinburgh, UKInstitute of Medical and Biological Problems of Russian Academy of SciencesMoscow, RussiaMicrobiology Unit, Belgian Nuclear Research Centre, SCK•CENMol, BelgiumCenter for Microbial Ecology and Technology, Ghent UniversityGhent, BelgiumMicrobe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term survival of Cupriavidus metallidurans CH34 in mineral water and the interaction with basalt, a lunar-type rock in preparation for the ESA spaceflight experiment, BIOROCK. Therefore, C. metallidurans CH34 cells were suspended in mineral water supplemented with or without crushed basalt and send for 3 months on board the Russian FOTON-M4 capsule. Long-term storage had a significant impact on cell physiology and energy status (by flow cytometry analysis, plate count and intracellular ATP measurements) as 60% of cells stored on ground lost their cell membrane potential, only 17% were still active, average ATP levels per cell were significantly lower and cultivability dropped to 1%. The cells stored in the presence of basalt and exposed to space flight conditions during storage however showed less dramatic changes in physiology, with only 16% of the cells lost their cell membrane potential and 24% were still active, leading to a higher cultivability (50%) and indicating a general positive effect of basalt and space flight on survival. Microbe-mineral interactions and biofilm formation was altered by spaceflight as less biofilm was formed on the basalt during flight conditions. Leaching from basalt also changed (measured with ICP-OES), showing that cells release more copper from basalt and the presence of cells also impacted iron and magnesium concentration irrespective of the presence of basalt. The flight conditions thus could counteract some of the detrimental effects observed after the 3 month storage conditions.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00671/fullmicrobe-mineral interactionsspace flightCupriavidus metallidurans CH34basaltFOTON |
| spellingShingle | Natalie Leys Bo Byloos Bo Byloos Ilse Coninx Olivier Van Hoey Charles Cockell Natasha Nicholson Vyacheslav Ilyin Rob Van Houdt Nico Boon The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock Frontiers in Microbiology microbe-mineral interactions space flight Cupriavidus metallidurans CH34 basalt FOTON |
| title | The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock |
| title_full | The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock |
| title_fullStr | The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock |
| title_full_unstemmed | The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock |
| title_short | The Impact of Space Flight on Survival and Interaction of Cupriavidus metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock |
| title_sort | impact of space flight on survival and interaction of cupriavidus metallidurans ch34 with basalt a volcanic moon analog rock |
| topic | microbe-mineral interactions space flight Cupriavidus metallidurans CH34 basalt FOTON |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00671/full |
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