Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells
Abstract Background During metastasis, tumor cells move through the tracks of extracellular matrix (ECM). Focal adhesions (FAs) are the protein complexes that link the cell cytoskeleton to the ECM and their presence is necessary for cell attachment. The tumor suppressor Nischarin interacts with a nu...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12943-018-0764-6 |
| _version_ | 1828364921855803392 |
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| author | Mazvita Maziveyi Shengli Dong Somesh Baranwal Suresh K. Alahari |
| author_facet | Mazvita Maziveyi Shengli Dong Somesh Baranwal Suresh K. Alahari |
| author_sort | Mazvita Maziveyi |
| collection | DOAJ |
| description | Abstract Background During metastasis, tumor cells move through the tracks of extracellular matrix (ECM). Focal adhesions (FAs) are the protein complexes that link the cell cytoskeleton to the ECM and their presence is necessary for cell attachment. The tumor suppressor Nischarin interacts with a number of signaling proteins such as Integrin α5, PAK1, LIMK1, LKB1, and Rac1 to prevent cancer cell migration. Although previous findings have shown that Nischarin exerts this migratory inhibition by interacting with other proteins, the effects of these interactions on the entire FA machinery are unknown. Methods RT-PCR, Western Blotting, invadopodia assays, and immunofluorescence were used to examine FA gene expression and determine whether Nischarin affects cell attachment, as well as the proteins that regulate it. Results Our data show that Nischarin prevents cell migration and invasion by altering the expression of key focal adhesion proteins. Furthermore, we have found that Nischarin-expressing cells have reduced ability to attach the ECM, which in turn leads to a decrease in invadopodia-mediated matrix degradation. Conclusions These experiments demonstrate an important role of Nischarin in regulating cell attachment, which adds to our understanding of the early events of the metastatic process in breast cancer. |
| first_indexed | 2024-04-14T05:23:34Z |
| format | Article |
| id | doaj.art-688578a69ba649708a76b3c15d44c821 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-14T05:23:34Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-688578a69ba649708a76b3c15d44c8212022-12-22T02:10:05ZengBMCMolecular Cancer1476-45982018-02-0117111110.1186/s12943-018-0764-6Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cellsMazvita Maziveyi0Shengli Dong1Somesh Baranwal2Suresh K. Alahari3Department of Biochemistry and Molecular Biology, LSUHSC School of MedicineDepartment of Biochemistry and Molecular Biology, LSUHSC School of MedicineDepartment of Biochemistry and Microbial Science, Central University of PunjabDepartment of Biochemistry and Molecular Biology, LSUHSC School of MedicineAbstract Background During metastasis, tumor cells move through the tracks of extracellular matrix (ECM). Focal adhesions (FAs) are the protein complexes that link the cell cytoskeleton to the ECM and their presence is necessary for cell attachment. The tumor suppressor Nischarin interacts with a number of signaling proteins such as Integrin α5, PAK1, LIMK1, LKB1, and Rac1 to prevent cancer cell migration. Although previous findings have shown that Nischarin exerts this migratory inhibition by interacting with other proteins, the effects of these interactions on the entire FA machinery are unknown. Methods RT-PCR, Western Blotting, invadopodia assays, and immunofluorescence were used to examine FA gene expression and determine whether Nischarin affects cell attachment, as well as the proteins that regulate it. Results Our data show that Nischarin prevents cell migration and invasion by altering the expression of key focal adhesion proteins. Furthermore, we have found that Nischarin-expressing cells have reduced ability to attach the ECM, which in turn leads to a decrease in invadopodia-mediated matrix degradation. Conclusions These experiments demonstrate an important role of Nischarin in regulating cell attachment, which adds to our understanding of the early events of the metastatic process in breast cancer.http://link.springer.com/article/10.1186/s12943-018-0764-6NischarinInvasionInvadopodiaMigrationFocal adhesionBreast cancer |
| spellingShingle | Mazvita Maziveyi Shengli Dong Somesh Baranwal Suresh K. Alahari Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells Molecular Cancer Nischarin Invasion Invadopodia Migration Focal adhesion Breast cancer |
| title | Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells |
| title_full | Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells |
| title_fullStr | Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells |
| title_full_unstemmed | Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells |
| title_short | Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells |
| title_sort | nischarin regulates focal adhesion and invadopodia formation in breast cancer cells |
| topic | Nischarin Invasion Invadopodia Migration Focal adhesion Breast cancer |
| url | http://link.springer.com/article/10.1186/s12943-018-0764-6 |
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