A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
ABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this stud...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2016-02-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00075-15 |
| _version_ | 1818750849613037568 |
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| author | Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides |
| author_facet | Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides |
| author_sort | Derek D. N. Smith |
| collection | DOAJ |
| description | ABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this study, we used the model host Dictyostelium discoideum to show that isolates of Pantoea ananatis exhibit differential grazing susceptibility, with some being resistant to grazing by the amoebae. We carried out a high-throughput genetic screen of one grazing-resistant isolate, P. ananatis BRT175, using the D. discoideum pathosystem to identify genes responsible for the resistance phenotype. Among the 26 candidate genes involved in grazing resistance, we identified rhlA and rhlB, which we show are involved in the biosynthesis of a biosurfactant that enables swarming motility in P. ananatis BRT175. Using liquid chromatography-mass spectrometry (LC-MS), the biosurfactant was shown to be a glycolipid with monohexose-C10-C10 as the primary congener. We show that this novel glycolipid biosurfactant is cytotoxic to the amoebae and is capable of compromising cellular integrity, leading to cell lysis. The production of this biosurfactant may be important for bacterial survival in the environment and could contribute to the establishment of opportunistic infections. IMPORTANCE The genetic factors used for host interaction by the opportunistic human pathogen Pantoea ananatis are largely unknown. We identified two genes that are important for the production of a biosurfactant that confers grazing resistance against the social amoeba Dictyostelium discoideum. We show that the biosurfactant, which exhibits cytotoxicity toward the amoebae, is a glycolipid that incorporates a hexose rather than rhamnose. The production of this biosurfactant may confer a competitive advantage in the environment and could potentially contribute to the establishment of opportunistic infections. |
| first_indexed | 2024-12-18T04:26:13Z |
| format | Article |
| id | doaj.art-6888926e78ae449f9b13ffd71be1d49c |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-18T04:26:13Z |
| publishDate | 2016-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-6888926e78ae449f9b13ffd71be1d49c2022-12-21T21:21:06ZengAmerican Society for MicrobiologymSphere2379-50422016-02-011110.1128/mSphere.00075-15A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>Derek D. N. Smith0Arvin Nickzad1Eric Déziel2John Stavrinides3Department of Biology, University of Regina, Regina, Saskatchewan, CanadaINRS-Institut Armand-Frappier, Laval, Québec, CanadaINRS-Institut Armand-Frappier, Laval, Québec, CanadaDepartment of Biology, University of Regina, Regina, Saskatchewan, CanadaABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this study, we used the model host Dictyostelium discoideum to show that isolates of Pantoea ananatis exhibit differential grazing susceptibility, with some being resistant to grazing by the amoebae. We carried out a high-throughput genetic screen of one grazing-resistant isolate, P. ananatis BRT175, using the D. discoideum pathosystem to identify genes responsible for the resistance phenotype. Among the 26 candidate genes involved in grazing resistance, we identified rhlA and rhlB, which we show are involved in the biosynthesis of a biosurfactant that enables swarming motility in P. ananatis BRT175. Using liquid chromatography-mass spectrometry (LC-MS), the biosurfactant was shown to be a glycolipid with monohexose-C10-C10 as the primary congener. We show that this novel glycolipid biosurfactant is cytotoxic to the amoebae and is capable of compromising cellular integrity, leading to cell lysis. The production of this biosurfactant may be important for bacterial survival in the environment and could contribute to the establishment of opportunistic infections. IMPORTANCE The genetic factors used for host interaction by the opportunistic human pathogen Pantoea ananatis are largely unknown. We identified two genes that are important for the production of a biosurfactant that confers grazing resistance against the social amoeba Dictyostelium discoideum. We show that the biosurfactant, which exhibits cytotoxicity toward the amoebae, is a glycolipid that incorporates a hexose rather than rhamnose. The production of this biosurfactant may confer a competitive advantage in the environment and could potentially contribute to the establishment of opportunistic infections.https://journals.asm.org/doi/10.1128/mSphere.00075-15biosurfactantsPantoeaDictyosteliumopportunistic infections |
| spellingShingle | Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> mSphere biosurfactants Pantoea Dictyostelium opportunistic infections |
| title | A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> |
| title_full | A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> |
| title_fullStr | A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> |
| title_full_unstemmed | A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> |
| title_short | A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content> |
| title_sort | novel glycolipid biosurfactant confers grazing resistance upon named content content type genus species pantoea ananatis named content brt175 against the social amoeba named content content type genus species dictyostelium discoideum named content |
| topic | biosurfactants Pantoea Dictyostelium opportunistic infections |
| url | https://journals.asm.org/doi/10.1128/mSphere.00075-15 |
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