Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake
Lingonberry (<i>Vaccinium vitis-idaea</i> L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics. The waste generated by the <i>V. vitis-idaea</i> processing industry is hard-skinned press cake th...
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MDPI AG
2022-09-01
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| Series: | Foods |
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| author | Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova |
| author_facet | Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova |
| author_sort | Daniil N. Olennikov |
| collection | DOAJ |
| description | Lingonberry (<i>Vaccinium vitis-idaea</i> L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics. The waste generated by the <i>V. vitis-idaea</i> processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far. In this study, water-soluble polysaccharides of <i>V. vitis-idaea</i> press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography. The results of elemental composition, monosaccharide analysis, ultraviolet–visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol–polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa. A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions. Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein–cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet. Polysaccharides and PPC of <i>V. vitis-idaea</i> fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders. |
| first_indexed | 2024-03-10T00:03:14Z |
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| language | English |
| last_indexed | 2024-03-10T00:03:14Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-6889b52cfd7d4be5836d237ddec53ec52023-11-23T16:13:26ZengMDPI AGFoods2304-81582022-09-011118280110.3390/foods11182801Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press CakeDaniil N. Olennikov0Vladimir V. Chemposov1Nadezhda K. Chirikova2Laboratory of Medical and Biological Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakhyanovoy Street, 670047 Ulan-Ude, RussiaDepartment of Biology, Institute of Natural Sciences, North-Eastern Federal University, 58 Belinsky Street, 677027 Yakutsk, RussiaDepartment of Biology, Institute of Natural Sciences, North-Eastern Federal University, 58 Belinsky Street, 677027 Yakutsk, RussiaLingonberry (<i>Vaccinium vitis-idaea</i> L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics. The waste generated by the <i>V. vitis-idaea</i> processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far. In this study, water-soluble polysaccharides of <i>V. vitis-idaea</i> press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography. The results of elemental composition, monosaccharide analysis, ultraviolet–visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol–polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa. A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions. Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein–cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet. Polysaccharides and PPC of <i>V. vitis-idaea</i> fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.https://www.mdpi.com/2304-8158/11/18/2801<i>Vaccinium vitis-idaea</i>lingonberrypolysaccharidespolyphenol–polysaccharide conjugatesarabinogalactangalacturonan |
| spellingShingle | Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake Foods <i>Vaccinium vitis-idaea</i> lingonberry polysaccharides polyphenol–polysaccharide conjugates arabinogalactan galacturonan |
| title | Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake |
| title_full | Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake |
| title_fullStr | Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake |
| title_full_unstemmed | Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake |
| title_short | Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from <i>Vaccinium vitis-idaea</i> Press Cake |
| title_sort | polymeric compounds of lingonberry waste characterization of antioxidant and hypolipidemic polysaccharides and polyphenol polysaccharide conjugates from i vaccinium vitis idaea i press cake |
| topic | <i>Vaccinium vitis-idaea</i> lingonberry polysaccharides polyphenol–polysaccharide conjugates arabinogalactan galacturonan |
| url | https://www.mdpi.com/2304-8158/11/18/2801 |
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