Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling
Introduction: The skeletal muscle deformity of commercial chickens (Gallus gallus), known as the wooden breast (WB), is associated with fibrotic myopathy of unknown etiology. For future breeding strategies and genetic improvements, it is essential to identify the molecular mechanisms underlying the...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1301804/full |
| _version_ | 1827595614567792640 |
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| author | Lucie Pejšková Sissel Beate Rønning Matthew Peter Kent Nina Therese Solberg Vibeke Høst To Thu-Hien Jens Petter Wold Marianne Lunde Ellen Mosleth Addolorata Pisconti Svein Olav Kolset Cathrine Rein Carlson Mona Elisabeth Pedersen |
| author_facet | Lucie Pejšková Sissel Beate Rønning Matthew Peter Kent Nina Therese Solberg Vibeke Høst To Thu-Hien Jens Petter Wold Marianne Lunde Ellen Mosleth Addolorata Pisconti Svein Olav Kolset Cathrine Rein Carlson Mona Elisabeth Pedersen |
| author_sort | Lucie Pejšková |
| collection | DOAJ |
| description | Introduction: The skeletal muscle deformity of commercial chickens (Gallus gallus), known as the wooden breast (WB), is associated with fibrotic myopathy of unknown etiology. For future breeding strategies and genetic improvements, it is essential to identify the molecular mechanisms underlying the phenotype. The pathophysiological hallmarks of WB include severe skeletal muscle fibrosis, inflammation, myofiber necrosis, and multifocal degeneration of muscle tissue. The transmembrane proteoglycans syndecans have a wide spectrum of biological functions and are master regulators of tissue homeostasis. They are upregulated and shed (cleaved) as a regulatory mechanism during tissue repair and regeneration. During the last decades, it has become clear that the syndecan family also has critical functions in skeletal muscle growth, however, their potential involvement in WB pathogenesis is unknown.Methods: In this study, we have categorized four groups of WB myopathy in broiler chickens and performed a comprehensive characterization of the molecular and histological profiles of two of them, with a special focus on the role of the syndecans and remodeling of the extracellular matrix (ECM).Results and discussion: Our findings reveal differential expression and shedding of the four syndecan family members and increased matrix metalloproteinase activity. Additionally, we identified alterations in key signaling pathways such as MAPK, AKT, and Wnt. Our work provides novel insights into a deeper understanding of WB pathogenesis and suggests potential therapeutic targets for this condition. |
| first_indexed | 2024-03-09T02:56:03Z |
| format | Article |
| id | doaj.art-688d56c30cbd467bb8636607d17eb62c |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-09T02:56:03Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-688d56c30cbd467bb8636607d17eb62c2023-12-05T05:11:34ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-12-011410.3389/fphys.2023.13018041301804Characterization of wooden breast myopathy: a focus on syndecans and ECM remodelingLucie Pejšková0Sissel Beate Rønning1Matthew Peter Kent2Nina Therese Solberg3Vibeke Høst4To Thu-Hien5Jens Petter Wold6Marianne Lunde7Ellen Mosleth8Addolorata Pisconti9Svein Olav Kolset10Cathrine Rein Carlson11Mona Elisabeth Pedersen12Raw Materials and Optimization, Nofima AS, Ås, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayCenter for Integrative Genetics, Department of Animal and Aquacultural Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), Ås, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayCenter for Integrative Genetics, Department of Animal and Aquacultural Sciences, Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), Ås, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayInstitute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayDepartment of Biochemistry and Cell Biology, Stony Brook, NY, United StatesDepartment of Nutrition, Institute of Basic Medical Science, University of Oslo, Oslo, NorwayInstitute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, NorwayRaw Materials and Optimization, Nofima AS, Ås, NorwayIntroduction: The skeletal muscle deformity of commercial chickens (Gallus gallus), known as the wooden breast (WB), is associated with fibrotic myopathy of unknown etiology. For future breeding strategies and genetic improvements, it is essential to identify the molecular mechanisms underlying the phenotype. The pathophysiological hallmarks of WB include severe skeletal muscle fibrosis, inflammation, myofiber necrosis, and multifocal degeneration of muscle tissue. The transmembrane proteoglycans syndecans have a wide spectrum of biological functions and are master regulators of tissue homeostasis. They are upregulated and shed (cleaved) as a regulatory mechanism during tissue repair and regeneration. During the last decades, it has become clear that the syndecan family also has critical functions in skeletal muscle growth, however, their potential involvement in WB pathogenesis is unknown.Methods: In this study, we have categorized four groups of WB myopathy in broiler chickens and performed a comprehensive characterization of the molecular and histological profiles of two of them, with a special focus on the role of the syndecans and remodeling of the extracellular matrix (ECM).Results and discussion: Our findings reveal differential expression and shedding of the four syndecan family members and increased matrix metalloproteinase activity. Additionally, we identified alterations in key signaling pathways such as MAPK, AKT, and Wnt. Our work provides novel insights into a deeper understanding of WB pathogenesis and suggests potential therapeutic targets for this condition.https://www.frontiersin.org/articles/10.3389/fphys.2023.1301804/fullwooden breastmyopathysyndecansextracellular matrixbroiler chicken |
| spellingShingle | Lucie Pejšková Sissel Beate Rønning Matthew Peter Kent Nina Therese Solberg Vibeke Høst To Thu-Hien Jens Petter Wold Marianne Lunde Ellen Mosleth Addolorata Pisconti Svein Olav Kolset Cathrine Rein Carlson Mona Elisabeth Pedersen Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling Frontiers in Physiology wooden breast myopathy syndecans extracellular matrix broiler chicken |
| title | Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling |
| title_full | Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling |
| title_fullStr | Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling |
| title_full_unstemmed | Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling |
| title_short | Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling |
| title_sort | characterization of wooden breast myopathy a focus on syndecans and ecm remodeling |
| topic | wooden breast myopathy syndecans extracellular matrix broiler chicken |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1301804/full |
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