Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts
<i>Sarcina</i> spp. has been isolated from the gastrointestinal tracts of diverse mammalian hosts. Their presence is often associated with host health complications, as is evident from many previously published medical case reports. However, only a handful of studies have made proper ide...
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MDPI AG
2023-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/9/1529 |
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| author | Marie Makovska Jiri Killer Nikol Modrackova Eugenio Ingribelli Ahmad Amin Eva Vlkova Petra Bolechova Vera Neuzil-Bunesova |
| author_facet | Marie Makovska Jiri Killer Nikol Modrackova Eugenio Ingribelli Ahmad Amin Eva Vlkova Petra Bolechova Vera Neuzil-Bunesova |
| author_sort | Marie Makovska |
| collection | DOAJ |
| description | <i>Sarcina</i> spp. has been isolated from the gastrointestinal tracts of diverse mammalian hosts. Their presence is often associated with host health complications, as is evident from many previously published medical case reports. However, only a handful of studies have made proper identification. Most other identifications were solely based on typical <i>Sarcina-</i>like morphology without genotyping. Therefore, the aim of this work was culture detection and the taxonomic classification of <i>Sarcina</i> isolates originating from different mammalian hosts. <i>Sarcina-</i>like colonies were isolated and collected during cultivation analyses of animal fecal samples (<i>n</i> = 197) from primates, dogs, calves of domestic cattle, elephants, and rhinoceroses. The study was carried out on apparently healthy animals kept in zoos or by breeders in the Czech Republic and Slovakia. Selected isolates were identified and compared using 16S rRNA gene sequencing and multi-locus sequence analysis (MLSA; <i>Iles, pheT, pyrG, rplB, rplC</i>, and <i>rpsC</i>). The results indicate the taxonomic variability of <i>Sarcina</i> isolates. <i>S. ventriculi</i> appears to be a common gut microorganism in various captive primates. In contrast, a random occurrence was also recorded in dogs. However, dog isolate N13/4e could represent the next potential novel <i>Sarcina</i> taxonomic unit. Also, a potentially novel <i>Sarcina</i> species was found in elephants, with occurrences in all tested hosts. <i>S. maxima</i> isolates were detected rarely, only in rhinoceroses. Although <i>Sarcina</i> bacteria are often linked to lethal diseases, our results indicate that <i>Sarcina</i> spp. appear to be a common member of the gut microbiota and seem to be an opportunistic pathogen. Further characterization and pathogenic analyses are required. |
| first_indexed | 2024-03-11T04:25:08Z |
| format | Article |
| id | doaj.art-688e05e95d3a408e92198cda0805d64a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T04:25:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-688e05e95d3a408e92198cda0805d64a2023-11-17T22:30:21ZengMDPI AGAnimals2076-26152023-05-01139152910.3390/ani13091529Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian HostsMarie Makovska0Jiri Killer1Nikol Modrackova2Eugenio Ingribelli3Ahmad Amin4Eva Vlkova5Petra Bolechova6Vera Neuzil-Bunesova7Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Ethology and Companion Animal Science, Faculty of Agrobiology, Food, and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, 165 00 Prague, Czech Republic<i>Sarcina</i> spp. has been isolated from the gastrointestinal tracts of diverse mammalian hosts. Their presence is often associated with host health complications, as is evident from many previously published medical case reports. However, only a handful of studies have made proper identification. Most other identifications were solely based on typical <i>Sarcina-</i>like morphology without genotyping. Therefore, the aim of this work was culture detection and the taxonomic classification of <i>Sarcina</i> isolates originating from different mammalian hosts. <i>Sarcina-</i>like colonies were isolated and collected during cultivation analyses of animal fecal samples (<i>n</i> = 197) from primates, dogs, calves of domestic cattle, elephants, and rhinoceroses. The study was carried out on apparently healthy animals kept in zoos or by breeders in the Czech Republic and Slovakia. Selected isolates were identified and compared using 16S rRNA gene sequencing and multi-locus sequence analysis (MLSA; <i>Iles, pheT, pyrG, rplB, rplC</i>, and <i>rpsC</i>). The results indicate the taxonomic variability of <i>Sarcina</i> isolates. <i>S. ventriculi</i> appears to be a common gut microorganism in various captive primates. In contrast, a random occurrence was also recorded in dogs. However, dog isolate N13/4e could represent the next potential novel <i>Sarcina</i> taxonomic unit. Also, a potentially novel <i>Sarcina</i> species was found in elephants, with occurrences in all tested hosts. <i>S. maxima</i> isolates were detected rarely, only in rhinoceroses. Although <i>Sarcina</i> bacteria are often linked to lethal diseases, our results indicate that <i>Sarcina</i> spp. appear to be a common member of the gut microbiota and seem to be an opportunistic pathogen. Further characterization and pathogenic analyses are required.https://www.mdpi.com/2076-2615/13/9/1529animalsmammaliansmicrobiota<i>Sarcina</i> spp.cultivationtaxonomy |
| spellingShingle | Marie Makovska Jiri Killer Nikol Modrackova Eugenio Ingribelli Ahmad Amin Eva Vlkova Petra Bolechova Vera Neuzil-Bunesova Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts Animals animals mammalians microbiota <i>Sarcina</i> spp. cultivation taxonomy |
| title | Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts |
| title_full | Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts |
| title_fullStr | Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts |
| title_full_unstemmed | Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts |
| title_short | Species and Strain Variability among <i>Sarcina</i> Isolates from Diverse Mammalian Hosts |
| title_sort | species and strain variability among i sarcina i isolates from diverse mammalian hosts |
| topic | animals mammalians microbiota <i>Sarcina</i> spp. cultivation taxonomy |
| url | https://www.mdpi.com/2076-2615/13/9/1529 |
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