Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.

In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty fi...

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Main Authors: FERNANDO J. SUTILI, MAURO A. CUNHA, ROSANGELA E. ZIECH, CARINA C. KREWER, CARLA C. ZEPPENFELD, CLARISSA G. HELDWEIN, LETICIA T. GRESSLER, BERTA M. HEINZMANN, AGUEDA C. VARGAS, BERNARDO BALDISSEROTTO
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2015-03-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=en
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author FERNANDO J. SUTILI
MAURO A. CUNHA
ROSANGELA E. ZIECH
CARINA C. KREWER
CARLA C. ZEPPENFELD
CLARISSA G. HELDWEIN
LETICIA T. GRESSLER
BERTA M. HEINZMANN
AGUEDA C. VARGAS
BERNARDO BALDISSEROTTO
author_facet FERNANDO J. SUTILI
MAURO A. CUNHA
ROSANGELA E. ZIECH
CARINA C. KREWER
CARLA C. ZEPPENFELD
CLARISSA G. HELDWEIN
LETICIA T. GRESSLER
BERTA M. HEINZMANN
AGUEDA C. VARGAS
BERNARDO BALDISSEROTTO
author_sort FERNANDO J. SUTILI
collection DOAJ
description In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.
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spelling doaj.art-688ec7dac4db4569b0233c4b780595942022-12-22T00:23:19ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-03-018719510010.1590/0001-3765201520130442S0001-37652015000100095Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.FERNANDO J. SUTILIMAURO A. CUNHAROSANGELA E. ZIECHCARINA C. KREWERCARLA C. ZEPPENFELDCLARISSA G. HELDWEINLETICIA T. GRESSLERBERTA M. HEINZMANNAGUEDA C. VARGASBERNARDO BALDISSEROTTOIn vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=enatividade antibacterianagentamicinaóleos essenciaispeixes
spellingShingle FERNANDO J. SUTILI
MAURO A. CUNHA
ROSANGELA E. ZIECH
CARINA C. KREWER
CARLA C. ZEPPENFELD
CLARISSA G. HELDWEIN
LETICIA T. GRESSLER
BERTA M. HEINZMANN
AGUEDA C. VARGAS
BERNARDO BALDISSEROTTO
Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
Anais da Academia Brasileira de Ciências
atividade antibacteriana
gentamicina
óleos essenciais
peixes
title Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
title_full Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
title_fullStr Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
title_full_unstemmed Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
title_short Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
title_sort lippia alba essential oil promotes survival of silver catfish rhamdia quelen infected with aeromonassp
topic atividade antibacteriana
gentamicina
óleos essenciais
peixes
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=en
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