Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.
In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty fi...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Academia Brasileira de Ciências
2015-03-01
|
Series: | Anais da Academia Brasileira de Ciências |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=en |
_version_ | 1818240913755865088 |
---|---|
author | FERNANDO J. SUTILI MAURO A. CUNHA ROSANGELA E. ZIECH CARINA C. KREWER CARLA C. ZEPPENFELD CLARISSA G. HELDWEIN LETICIA T. GRESSLER BERTA M. HEINZMANN AGUEDA C. VARGAS BERNARDO BALDISSEROTTO |
author_facet | FERNANDO J. SUTILI MAURO A. CUNHA ROSANGELA E. ZIECH CARINA C. KREWER CARLA C. ZEPPENFELD CLARISSA G. HELDWEIN LETICIA T. GRESSLER BERTA M. HEINZMANN AGUEDA C. VARGAS BERNARDO BALDISSEROTTO |
author_sort | FERNANDO J. SUTILI |
collection | DOAJ |
description | In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%. |
first_indexed | 2024-12-12T13:21:00Z |
format | Article |
id | doaj.art-688ec7dac4db4569b0233c4b78059594 |
institution | Directory Open Access Journal |
issn | 1678-2690 |
language | English |
last_indexed | 2024-12-12T13:21:00Z |
publishDate | 2015-03-01 |
publisher | Academia Brasileira de Ciências |
record_format | Article |
series | Anais da Academia Brasileira de Ciências |
spelling | doaj.art-688ec7dac4db4569b0233c4b780595942022-12-22T00:23:19ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-03-018719510010.1590/0001-3765201520130442S0001-37652015000100095Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp.FERNANDO J. SUTILIMAURO A. CUNHAROSANGELA E. ZIECHCARINA C. KREWERCARLA C. ZEPPENFELDCLARISSA G. HELDWEINLETICIA T. GRESSLERBERTA M. HEINZMANNAGUEDA C. VARGASBERNARDO BALDISSEROTTOIn vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=enatividade antibacterianagentamicinaóleos essenciaispeixes |
spellingShingle | FERNANDO J. SUTILI MAURO A. CUNHA ROSANGELA E. ZIECH CARINA C. KREWER CARLA C. ZEPPENFELD CLARISSA G. HELDWEIN LETICIA T. GRESSLER BERTA M. HEINZMANN AGUEDA C. VARGAS BERNARDO BALDISSEROTTO Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. Anais da Academia Brasileira de Ciências atividade antibacteriana gentamicina óleos essenciais peixes |
title | Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. |
title_full | Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. |
title_fullStr | Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. |
title_full_unstemmed | Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. |
title_short | Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. |
title_sort | lippia alba essential oil promotes survival of silver catfish rhamdia quelen infected with aeromonassp |
topic | atividade antibacteriana gentamicina óleos essenciais peixes |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100095&lng=en&tlng=en |
work_keys_str_mv | AT fernandojsutili lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT mauroacunha lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT rosangelaeziech lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT carinackrewer lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT carlaczeppenfeld lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT clarissagheldwein lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT leticiatgressler lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT bertamheinzmann lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT aguedacvargas lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp AT bernardobaldisserotto lippiaalbaessentialoilpromotessurvivalofsilvercatfishrhamdiaqueleninfectedwithaeromonassp |