Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion
The aim of this study was to evaluate in vitro the anti-inflammatory activity of phenolic acids absorbed through a Caco-2 monolayer after simulated digestion of spray-dried nejayote powder. Mouse macrophages (Raw 264.7) were treated with the basolateral fractions obtained at 2, 4, 8, or 12 h from th...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-12-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617305820 |
| _version_ | 1818617663044190208 |
|---|---|
| author | Javier Villela-Castrejón Marilena Antunes-Ricardo Janet A. Gutiérrez-Uribe |
| author_facet | Javier Villela-Castrejón Marilena Antunes-Ricardo Janet A. Gutiérrez-Uribe |
| author_sort | Javier Villela-Castrejón |
| collection | DOAJ |
| description | The aim of this study was to evaluate in vitro the anti-inflammatory activity of phenolic acids absorbed through a Caco-2 monolayer after simulated digestion of spray-dried nejayote powder. Mouse macrophages (Raw 264.7) were treated with the basolateral fractions obtained at 2, 4, 8, or 12 h from the Caco-2 monolayer permeability experiment to measure the effects on NF-κB, TNF-α, COX-2, interleukin-10 (IL-10), and nitric oxide (NO) levels. When the digested samples were exposed to a Caco-2 monolayer, 67% of total ferulic acid (FA) present in spray-dried nejayote powder was recovered in the basolateral fractions after 8 h. Phenolic acids recovered after 2 h exerted the highest anti-inflammatory activity by inhibiting 53.8%, 93.4%, 86.3% and 84.7% the production of NF-κB, TNF-α, COX-2, and NO, respectively. Future in vivo studies should be designed to reach less than 7 μM of FA and its derivatives to assess the potential health benefits. |
| first_indexed | 2024-12-16T17:09:16Z |
| format | Article |
| id | doaj.art-6892a3458b354b739b28b951a2889f11 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T17:09:16Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6892a3458b354b739b28b951a2889f112022-12-21T22:23:27ZengElsevierJournal of Functional Foods1756-46462017-12-01393743Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestionJavier Villela-Castrejón0Marilena Antunes-Ricardo1Janet A. Gutiérrez-Uribe2Tecnologico de Monterrey, Centro de Biotecnología-FEMSA, School of Engineering and Sciences, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, MexicoTecnologico de Monterrey, Centro de Biotecnología-FEMSA, School of Engineering and Sciences, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, MexicoCorresponding author.; Tecnologico de Monterrey, Centro de Biotecnología-FEMSA, School of Engineering and Sciences, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, MexicoThe aim of this study was to evaluate in vitro the anti-inflammatory activity of phenolic acids absorbed through a Caco-2 monolayer after simulated digestion of spray-dried nejayote powder. Mouse macrophages (Raw 264.7) were treated with the basolateral fractions obtained at 2, 4, 8, or 12 h from the Caco-2 monolayer permeability experiment to measure the effects on NF-κB, TNF-α, COX-2, interleukin-10 (IL-10), and nitric oxide (NO) levels. When the digested samples were exposed to a Caco-2 monolayer, 67% of total ferulic acid (FA) present in spray-dried nejayote powder was recovered in the basolateral fractions after 8 h. Phenolic acids recovered after 2 h exerted the highest anti-inflammatory activity by inhibiting 53.8%, 93.4%, 86.3% and 84.7% the production of NF-κB, TNF-α, COX-2, and NO, respectively. Future in vivo studies should be designed to reach less than 7 μM of FA and its derivatives to assess the potential health benefits.http://www.sciencedirect.com/science/article/pii/S1756464617305820Ferulic acidInflammationCaco-2 monolayerNejayotePermeability |
| spellingShingle | Javier Villela-Castrejón Marilena Antunes-Ricardo Janet A. Gutiérrez-Uribe Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion Journal of Functional Foods Ferulic acid Inflammation Caco-2 monolayer Nejayote Permeability |
| title | Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion |
| title_full | Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion |
| title_fullStr | Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion |
| title_full_unstemmed | Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion |
| title_short | Bioavailability and anti-inflammatory activity of phenolic acids found in spray-dried nejayote after its in vitro digestion |
| title_sort | bioavailability and anti inflammatory activity of phenolic acids found in spray dried nejayote after its in vitro digestion |
| topic | Ferulic acid Inflammation Caco-2 monolayer Nejayote Permeability |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617305820 |
| work_keys_str_mv | AT javiervillelacastrejon bioavailabilityandantiinflammatoryactivityofphenolicacidsfoundinspraydriednejayoteafteritsinvitrodigestion AT marilenaantunesricardo bioavailabilityandantiinflammatoryactivityofphenolicacidsfoundinspraydriednejayoteafteritsinvitrodigestion AT janetagutierrezuribe bioavailabilityandantiinflammatoryactivityofphenolicacidsfoundinspraydriednejayoteafteritsinvitrodigestion |