Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol
ABSTRACT: Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was dev...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124000270 |
| _version_ | 1797291832037605376 |
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| author | Hsiu-Lien Herbie Lin Pascal Mermillod Isabelle Grasseau Elisabeth Blesbois Anaïs Vitorino Carvalho |
| author_facet | Hsiu-Lien Herbie Lin Pascal Mermillod Isabelle Grasseau Elisabeth Blesbois Anaïs Vitorino Carvalho |
| author_sort | Hsiu-Lien Herbie Lin |
| collection | DOAJ |
| description | ABSTRACT: Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was developed to eliminate glycerol and mitigate its adverse effects. We have recently developed a new method for removing glycerol called sucrose-Percoll (SP), that can be performed at either 4°C (4°C-SP) or 20°C (20°C-SP). This SP protocol has been found to be simpler and faster to improve fertility compared to the traditional SD method. Nevertheless, the reasons for such effectiveness differences between glycerol removal procedures remained unclear and required more comprehensive understandings for future protocol developments. Here, we examined the effects of SP and SD protocols on the fertility duration. We also investigated the potential causes of varying effects of these methods by analyzing sperm quality parameters and sperm storage in the hen's reproductive tract. The fertility was significantly higher in 4°C-SP than 20°C-SP during the first 6 d after insemination, and also higher than sperm processed using SD. No difference was observed between 20°C-SP and SD between 7 and 13 d. However, a 2.7-time higher fertility was shown with 4°C-SP. In addition, the SP method demonstrated a 2-fold greater ability to remove glycerol than the SD method. Sperm centrifuged at 4°C-SP exhibited higher sperm storage compared to 20°C-SP and were higher than sperm treated with SD. Overall, our findings revealed that the differences in efficiencies between SP and SD methods were not related to in vitro sperm quality but resulted from a higher ability to remove glycerol, a higher storage capacity in the female reproductive tract, and a longer fertility ability. Since no impacts were observed in sperm cellular characteristics, further experiments are necessary to investigate the influences of glycerol removal treatments at the molecular level. |
| first_indexed | 2024-03-07T19:43:26Z |
| format | Article |
| id | doaj.art-68957936de434ac98a7314d7121c3c02 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-07T19:43:26Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-68957936de434ac98a7314d7121c3c022024-02-29T05:17:25ZengElsevierPoultry Science0032-57912024-03-011033103448Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerolHsiu-Lien Herbie Lin0Pascal Mermillod1Isabelle Grasseau2Elisabeth Blesbois3Anaïs Vitorino Carvalho4INRAe, CNRS, IFCE, Université de Tours, PRC, 37380, Nouzilly, France; Division of Physiology, LRI, COA, 71246, Tainan, TaiwanINRAe, CNRS, IFCE, Université de Tours, PRC, 37380, Nouzilly, FranceINRAe, CNRS, IFCE, Université de Tours, PRC, 37380, Nouzilly, FranceINRAe, CNRS, IFCE, Université de Tours, PRC, 37380, Nouzilly, FranceINRAe, CNRS, IFCE, Université de Tours, PRC, 37380, Nouzilly, France; Corresponding author:ABSTRACT: Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was developed to eliminate glycerol and mitigate its adverse effects. We have recently developed a new method for removing glycerol called sucrose-Percoll (SP), that can be performed at either 4°C (4°C-SP) or 20°C (20°C-SP). This SP protocol has been found to be simpler and faster to improve fertility compared to the traditional SD method. Nevertheless, the reasons for such effectiveness differences between glycerol removal procedures remained unclear and required more comprehensive understandings for future protocol developments. Here, we examined the effects of SP and SD protocols on the fertility duration. We also investigated the potential causes of varying effects of these methods by analyzing sperm quality parameters and sperm storage in the hen's reproductive tract. The fertility was significantly higher in 4°C-SP than 20°C-SP during the first 6 d after insemination, and also higher than sperm processed using SD. No difference was observed between 20°C-SP and SD between 7 and 13 d. However, a 2.7-time higher fertility was shown with 4°C-SP. In addition, the SP method demonstrated a 2-fold greater ability to remove glycerol than the SD method. Sperm centrifuged at 4°C-SP exhibited higher sperm storage compared to 20°C-SP and were higher than sperm treated with SD. Overall, our findings revealed that the differences in efficiencies between SP and SD methods were not related to in vitro sperm quality but resulted from a higher ability to remove glycerol, a higher storage capacity in the female reproductive tract, and a longer fertility ability. Since no impacts were observed in sperm cellular characteristics, further experiments are necessary to investigate the influences of glycerol removal treatments at the molecular level.http://www.sciencedirect.com/science/article/pii/S0032579124000270cryopreservationfertilityglycerolsperm storage tubulestoxicity |
| spellingShingle | Hsiu-Lien Herbie Lin Pascal Mermillod Isabelle Grasseau Elisabeth Blesbois Anaïs Vitorino Carvalho Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol Poultry Science cryopreservation fertility glycerol sperm storage tubules toxicity |
| title | Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| title_full | Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| title_fullStr | Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| title_full_unstemmed | Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| title_short | Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| title_sort | exploring how sucrose colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol |
| topic | cryopreservation fertility glycerol sperm storage tubules toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124000270 |
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