Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection
To identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (<b>1</b>), 2′-hydroxyl neophellamuretin (<b>2</b>), yokovanol (<b>3</b>), swertisin (<b>4</b>), spinosin (<b>5</b>), and 7-methyl-apigeni...
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MDPI AG
2020-09-01
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Online Access: | https://www.mdpi.com/1420-3049/25/19/4387 |
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author | Eun Bin Kwon Hye Jin Yang Jang-Gi Choi Wei Li |
author_facet | Eun Bin Kwon Hye Jin Yang Jang-Gi Choi Wei Li |
author_sort | Eun Bin Kwon |
collection | DOAJ |
description | To identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (<b>1</b>), 2′-hydroxyl neophellamuretin (<b>2</b>), yokovanol (<b>3</b>), swertisin (<b>4</b>), spinosin (<b>5</b>), and 7-methyl-apigenin-6-C-β-glucopyranosyl 2″-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-xylopyranoside (<b>6</b>) from MeOH extractions of <i>Ohwia caudata</i>. We screened these compounds for antiviral activity using green fluorescent protein (GFP)-expressing H1N1 (A/PR/8/34) influenza A-infected RAW 264.7 cells. Compounds <b>1</b> and <b>3</b> exhibited significant inhibitory effects against influenza A viral infection in co-treatment conditions. In addition, compounds <b>1</b> and <b>3</b> reduced viral protein levels, including M1, M2, HA, and neuraminidase (NA), and suppressed neuraminidase (NA) activity in RAW 264.7 cells. These findings demonstrated that 2′-hydroxyl yokovanol and yokovanol, isolated from <i>O. caudate</i>, inhibit influenza A virus by suppressing NA activity. The moderate inhibitory activities of these flavonoids against influenza A virus suggest that they may be developed as novel anti-influenza drugs in the future. |
first_indexed | 2024-03-10T16:05:41Z |
format | Article |
id | doaj.art-6895efef50bc43528b4260d890186a59 |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-10T16:05:41Z |
publishDate | 2020-09-01 |
publisher | MDPI AG |
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series | Molecules |
spelling | doaj.art-6895efef50bc43528b4260d890186a592023-11-20T14:56:46ZengMDPI AGMolecules1420-30492020-09-012519438710.3390/molecules25194387Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus InfectionEun Bin Kwon0Hye Jin Yang1Jang-Gi Choi2Wei Li3Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaTo identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (<b>1</b>), 2′-hydroxyl neophellamuretin (<b>2</b>), yokovanol (<b>3</b>), swertisin (<b>4</b>), spinosin (<b>5</b>), and 7-methyl-apigenin-6-C-β-glucopyranosyl 2″-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-xylopyranoside (<b>6</b>) from MeOH extractions of <i>Ohwia caudata</i>. We screened these compounds for antiviral activity using green fluorescent protein (GFP)-expressing H1N1 (A/PR/8/34) influenza A-infected RAW 264.7 cells. Compounds <b>1</b> and <b>3</b> exhibited significant inhibitory effects against influenza A viral infection in co-treatment conditions. In addition, compounds <b>1</b> and <b>3</b> reduced viral protein levels, including M1, M2, HA, and neuraminidase (NA), and suppressed neuraminidase (NA) activity in RAW 264.7 cells. These findings demonstrated that 2′-hydroxyl yokovanol and yokovanol, isolated from <i>O. caudate</i>, inhibit influenza A virus by suppressing NA activity. The moderate inhibitory activities of these flavonoids against influenza A virus suggest that they may be developed as novel anti-influenza drugs in the future.https://www.mdpi.com/1420-3049/25/19/4387<i>Ohwia caudata</i>flavonoidinfluenza Aneuraminidase |
spellingShingle | Eun Bin Kwon Hye Jin Yang Jang-Gi Choi Wei Li Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection Molecules <i>Ohwia caudata</i> flavonoid influenza A neuraminidase |
title | Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection |
title_full | Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection |
title_fullStr | Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection |
title_full_unstemmed | Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection |
title_short | Protective Effect of Flavonoids from <i>Ohwia caudata</i> against Influenza a Virus Infection |
title_sort | protective effect of flavonoids from i ohwia caudata i against influenza a virus infection |
topic | <i>Ohwia caudata</i> flavonoid influenza A neuraminidase |
url | https://www.mdpi.com/1420-3049/25/19/4387 |
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